FastQCFastQC Report
Sat 31 Dec 2022
SRR5847270.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847270.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221547
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC6370.2875236405819081No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT5770.2604413510451507RNA PCR Primer, Index 23 (95% over 21bp)
CGAATAGGGCTATGGAGATCTGGATAAATTACATTTTATATTTGATTTTGAT2850.12864087529959783No Hit
CTTCATGCGGGCTTGAGTTCTCGGCAATAATGAGTGATTATTGTTATTTTTA2850.12864087529959783No Hit
AAGTTAGGGAGGAAAGGTTGGCCAAAGTTTTTATGTTTCTTTTTTATTTTCT2670.12051618843857059No Hit
TGCTATGGGGAGCCTGACCAACATGGAGAAACCCCGTCTCTACTAAAAATAC2650.11961344545401201No Hit
ACACAAGGGCCTCTGCCTCCCGGGTTCAAGTGATTCTCCTGCCTCAGCCTCC2650.11961344545401201No Hit
AAGAATGGGATTGGTAAGATGTGAAGTCCCAATGAAAGGCAGCTTCTGATTT2640.11916207396173273No Hit
CAATTGGGACAACTGTCCCTTGGCTGTGATCATTTTTCAGTTTGCCCCACAG2630.11871070246945344No Hit
TCCAACGGGACTGGTCTGCTTGAGTGAATACACTGGAAAAAGAGGGGAAATG2620.11825933097717414No Hit
ATTCTTGGGGAATGAGGGAGAGAGGGAACGGGAGAAGGAGGGATGGAAGTAG2620.11825933097717414No Hit
TCTAGTGGGTTCAAATGAGAGAATAAGACCACTTGGTGTCTAAAGTCTTTCT2590.11690521650033628No Hit
ACAACTGGGAACCCTGATGCTGCTAGCCGGGCTATTGATTATGTACAGAGAC2560.1155511020234984No Hit
CAATGCGGGATGGGGGTTCCTTAAGATTTCAAAACTGCTGTATGATCTGGCT2540.11464835903893981No Hit
CACAGTGGGATAGGCATATTTTCAGCCAATTCAGCTTAATTCAAACATAACT2540.11464835903893981No Hit
CTCTCGGGGGGAGCAAAAATGACAAGTTTTACTAGCTAAGTAGAGAAATAAA2520.11374561605438124No Hit
AGCAATGGGGAAAGAAATGAAATGGGAAGCAAACAAACAGAAGATCAAAAAA2520.11374561605438124No Hit
TTGCTTGGGGCCCCAGTCACTGAGCCGCCGCCGAGGATTCAGCAGCCTCCCC2520.11374561605438124No Hit
GTGGCTGGGGGACTCCGCCCAGCGAGGCGGAGCCTAGGGAAGCCGCGGGCGG2500.11284287306982266No Hit
AAATAAGGGAACAACTTTTATCTATCAATTTCCATTACAGAATCAAGAATAT2500.11284287306982266No Hit
GACAGGGGCAAAATTTAAACTTAGAGTTGTGCTTCTAGAAATGGAAGCTTTT2470.11148875859298478No Hit
ATTTCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2460.11103738710070549No Hit
TCGTCAGGGATCCAGACTTTATTTTTCCACTTAGACAGCACAGGCAGAGTGC2460.11103738710070549No Hit
ATCAATGGGATAGTTGCTCAGTGACAAAGCAAAGGAAGTTTAATATTTTCCA2450.11058601560842621No Hit
TCTTCTGGGTATGAAAACAAGTGTGGGCATTTTCAAAGGCCCGAGTGTGTCC2450.11058601560842621No Hit
GGTGGTGGGTCTTGCGAAGGGATTGTAGTTTTGATTTGCATTTCCCAGATCA2440.11013464411614692No Hit
ACACACGGCGATCTTTGGCGGGATATGCAAAATGGATTTGGGTTTTTGAGAT2440.11013464411614692No Hit
GTGAATGGGCGACTTGCTTTTGTATCAGGACGAGGAGGCGCTGAACGACTCC2440.11013464411614692No Hit
GGTATGGGACCTTAGGGAAGTCCCAGACAGTTAACAATTTAACTAATGCCAC2440.11013464411614692No Hit
GAGAATGGGGAAGGTGGAGCCTTTGAGAGGTGATCGGGTCACGAAGACAGAG2430.10968327262386762No Hit
AGTCTTGGGAACCATGAAGTTTCTTTTAAAGCTGAGCTGTATTTCTTCCCAG2420.10923190113158833No Hit
GGAACGGGGGACTTAAGAATGTATTTTCAGCCCAAGGAACAATTTTCCTGTT2410.10878052963930905No Hit
CCGCTAGGGATGCCCGGCTAATTTATTTTTAGTAGAGATGAGGTTTCACCTT2410.10878052963930905No Hit
CGTTTTGGGATTACCCAAATTATTCAAGAAAATAGATCAGAAATAAAGAACA2400.10832915814702976No Hit
GAAACTGGGGATTGTTAAAATTGTAATGCATTACATTTTAATCTACAAATAA2390.10787778665475045No Hit
TGGTTCGGGAGGTGGGTTACTGGTAGATGATAGTGGTGACACCTGCAGGTCT2390.10787778665475045No Hit
GACTTAGGGACCCGCTGAACTGAAATCTCCTTTCCAGAGGGCAGAGTGGTCC2380.10742641516247116No Hit
AGCTTTGGGACTACTTGGGGGGCTGATGTGGGGGGATTGCTTGAGCCCAGGA2380.10742641516247116No Hit
AAGACTTGGGAGCAACGTGAAAACAGACAAATGCAAGTATCAAATAAATGTC2380.10742641516247116No Hit
GAACCCGGGATTGAGGTGAAAGGAGCACAGAAGGACTTTGAAGAAATAAATT2360.10652367217791259No Hit
CACAACGGGAATGTATCGCAAATTCACAGGTTTCAATATGAGGAGATAATTT2360.10652367217791259No Hit
TGCCAAGGGAATATTTGAAAATCTAGAGCAGAAATGATAAGTTGCATCTCAA2350.1060723006856333No Hit
TCAAACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2350.1060723006856333No Hit
TGGGAAGGGAGGAAAAGCTGGTTTCTATTATATGATTTTCCTACATATGGCA2350.1060723006856333No Hit
GAATCTGGGGTCCTTAGTATCTTACACTTAGGAGAGATATTATTACTCAATA2350.1060723006856333No Hit
AGTGAGGGGAGAAGACACTTCCCTGAGCCAGCAGAAGGGACAGGTGCAGCAG2340.105620929193354No Hit
TATAATGGGTGACAGCACATTGCCAGGAAGCAGGCCTGTAGTGTAAATTGTT2340.105620929193354No Hit
AGAAAAGGGATTGAGGAAATTGGGTGTTGGATGCAAAGCTACAGCACGTCTT2330.10516955770107472No Hit
ACGAATGGGGTAGTTGCAGCTGATGCCTGAATGTATCCTAGCTGACAAATTA2330.10516955770107472No Hit
TACTTTGGGATTGTTGGGCTATTTGCATGATTTCCTAAAATATCTGGCAAAG2310.10426681471651614No Hit
TTCGTAGGGGCTTGGAGAATAAATGAGTGAATGAATGCATGGATAAAATTGG2310.10426681471651614No Hit
AGTGTCGGGGCTAGCAATGAAGTCCAGAAGTTGTCAATGGTTCATCCCCAGA2300.10381544322423686No Hit
CACAAAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2300.10381544322423686No Hit
TATTGCGGGGTCATTGCTCTGTTTTTAATTTAGAGTGGTTATGGAAGGTACA2300.10381544322423686No Hit
AAAGTAGGGGCTTTTGAAAAAGGCTAATCTGTTAATTTCCCCAGTATTCTTT2300.10381544322423686No Hit
AATCAGGGTCGCTTTGTGTGGTCTGATAAATACATTGATATTTAGGTTGAAA2290.10336407173195754No Hit
ACATTAGGGATAGTTGTATAAAAGAAATACACAGCACATTCTTGTTGTGTGT2290.10336407173195754No Hit
ATTAAAGGGTGCATTTTTGTACCTGAGTTGGAGTGACATGGCACAGATGGCA2290.10336407173195754No Hit
GCGTTAGGGACATTAAAATAAGTACATGTGTTGATACACACATACCACATAA2280.10291270023967826No Hit
TCTGATGGGCAGTTAAGTCATTTTAGACATAGCATTTATTATCACTGTGGAT2280.10291270023967826No Hit
TGCTCTGGGAGTCAGTCTAGCTAAACGTTTGTCAATTTTGTTTCTCTTTTCA2280.10291270023967826No Hit
TCACTAGGGGCAACCGGTCCCGCTCTCGCACCCTGTCCCAGAGCAGCGAGTC2280.10291270023967826No Hit
CTACCCGGGTTGAAGCTGGAGGTACAAAGTAAAAGTCCGCCAAACCCATCCC2270.10246132874739897No Hit
GCATAGGGGCAGAATTCTACCAGACATTGAAAGAAGAGTTGGTATCAATCCT2260.10200995725511967No Hit
CCTGTAGGGATAAATGAATGAGCATGGCTATGTTCCAATAAAACTTAATTGA2260.10200995725511967No Hit
CACCAGGGGATGATGAAACCACTTAAAAAGTCTAACAGTAATAACAACAGCA2260.10200995725511967No Hit
GCAAATGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATG2260.10200995725511967No Hit
TAACACGGGATTTCCTGCCTGAGTCCTGGAATCAACCGTTTCTCTAGGGAGG2250.1015585857628404No Hit
CAGTTGGGGATTCTTAACTCATTCTGTGAGGTCAGAATTATAATCATCGCCA2250.1015585857628404No Hit
CATTAGGGGATGCTTTTTATAGGCAGTTTTATTTCTTCTCTTATTCTTCCTT2230.10065584277828181No Hit
TGGTCCCGGGAGAATAGAACACCACTACTTCATTGGCACCAGAGGGAAGCAG2220.10020447128600253No Hit
AGGAATAGGGTCTCGTGCCGGGGATCTATACGGGGGTAACCAAATAATTTTC2220.10020447128600253No Hit
GGGATGCTAGGGTAATTTAAAAACACAAAAAAAATTTTAGCTCTTCCCACTT2220.10020447128600253No Hit
GCTTATGGGAAGAGATTATTATTTTGCTCTTCATGGACCTGTTAACAACCAC2220.10020447128600253No Hit
AAACTAGGGTCAAGTGGACAAGTGATTTGAAATCAGCCATAGTGATTTACTT2220.10020447128600253No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)