FastQCFastQC Report
Sat 31 Dec 2022
SRR5847269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221345
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC6220.28100928414917886No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT6090.275136099753778RNA PCR Primer, Index 23 (95% over 21bp)
AAGTTAGGGAGGAAAGGTTGGCCAAAGTTTTTATGTTTCTTTTTTATTTTCT2860.12921005669881858No Hit
CGAATAGGGCTATGGAGATCTGGATAAATTACATTTTATATTTGATTTTGAT2740.12378865571844858No Hit
ACACAAGGGCCTCTGCCTCCCGGGTTCAAGTGATTCTCCTGCCTCAGCCTCC2700.12198152205832524No Hit
CCGCTAGGGATGCCCGGCTAATTTATTTTTAGTAGAGATGAGGTTTCACCTT2680.12107795522826358No Hit
TCCAACGGGACTGGTCTGCTTGAGTGAATACACTGGAAAAAGAGGGGAAATG2650.11972260498317105No Hit
CAATGCGGGATGGGGGTTCCTTAAGATTTCAAAACTGCTGTATGATCTGGCT2640.11927082156814024No Hit
AAGACTTGGGAGCAACGTGAAAACAGACAAATGCAAGTATCAAATAAATGTC2630.1188190381531094No Hit
AAGAATGGGATTGGTAAGATGTGAAGTCCCAATGAAAGGCAGCTTCTGATTT2610.11791547132304772No Hit
CTTCATGCGGGCTTGAGTTCTCGGCAATAATGAGTGATTATTGTTATTTTTA2580.11656012107795523No Hit
AGTGAGGGGAGAAGACACTTCCCTGAGCCAGCAGAAGGGACAGGTGCAGCAG2570.11610833766292439No Hit
TGTGTTGGGGCTTTTTTAAGATAATCTAAAACTTTTCCTTGAACGATGCTGT2500.11294585375770856No Hit
TGGGAAGGGAGGAAAAGCTGGTTTCTATTATATGATTTTCCTACATATGGCA2480.11204228692764688No Hit
CACAGTGGGATAGGCATATTTTCAGCCAATTCAGCTTAATTCAAACATAACT2470.11159050351261605No Hit
CTTGCTGGGTACGGCCAAGTTTCTTCTAGTATGATGGAAAGTTTCTTTTTTC2460.11113872009758521No Hit
AGCAATGGGGAAAGAAATGAAATGGGAAGCAAACAAACAGAAGATCAAAAAA2450.11068693668255439No Hit
GGTGGTGGGTCTTGCGAAGGGATTGTAGTTTTGATTTGCATTTCCCAGATCA2430.10978336985249272No Hit
AAGCATGGGAGATCTGACTTGTGGCAAACTTAGTGTTTGCTGTATGCCAAAT2430.10978336985249272No Hit
GCTCCTGGGATATCCTGGATTTCTGAACTGGTCTTGTTGACAAGGATTCCCA2430.10978336985249272No Hit
TTGCTTGGGGCCCCAGTCACTGAGCCGCCGCCGAGGATTCAGCAGCCTCCCC2430.10978336985249272No Hit
ACAACTGGGAACCCTGATGCTGCTAGCCGGGCTATTGATTATGTACAGAGAC2420.10933158643746188No Hit
TACAGGGGCTGCAAGTGGGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.10933158643746188No Hit
TTAGTTGGGATCATCTAATCCTCTAAAGGCCTGGATAGAACAAAGGGCAGAG2400.1084280196074002No Hit
CCTGTAGGGATAAATGAATGAGCATGGCTATGTTCCAATAAAACTTAATTGA2400.1084280196074002No Hit
ACTCATGGGCTCATTTTCACACCTAGCTGTTATCTCTTCAGAATTTCCAAGC2390.10797623619236939No Hit
TATCGGGATCTGTGATATGAAAACTGTTGGAGATACAAGAAAACAGACACAG2390.10797623619236939No Hit
ACGAATGGGGTAGTTGCAGCTGATGCCTGAATGTATCCTAGCTGACAAATTA2390.10797623619236939No Hit
TCTTCTGGGTATGAAAACAAGTGTGGGCATTTTCAAAGGCCCGAGTGTGTCC2390.10797623619236939No Hit
TACTTTGGGATTGTTGGGCTATTTGCATGATTTCCTAAAATATCTGGCAAAG2390.10797623619236939No Hit
CTTTACTGGGACATAATTTGAATCAACACAACCACCCACAGCCTAATTATTA2390.10797623619236939No Hit
TCTAGTGGGTTCAGGGCACAGGGGCCCTGGGACGTGGTTGGCAGCCAGGCTG2390.10797623619236939No Hit
TTATACGGGAGTCCTTCTGGTCAGTGAACGAAAATTCTAGACCTACAGTTAC2370.1070726693623077No Hit
TCGTCAGGGATCCAGACTTTATTTTTCCACTTAGACAGCACAGGCAGAGTGC2370.1070726693623077No Hit
TCATGCGGGATGCATATATGTAGTGAAGTAATTACTACAGTCAAGTAAATTA2360.10662088594727687No Hit
AAGTGGGGCTCAAGGACACACAAGCAGGACAAGCTGGCAGAGGAATCTAAGT2360.10662088594727687No Hit
GAAACTGGGGATTGTTAAAATTGTAATGCATTACATTTTAATCTACAAATAA2360.10662088594727687No Hit
CAGTTGGGGATTCTTAACTCATTCTGTGAGGTCAGAATTATAATCATCGCCA2360.10662088594727687No Hit
CATTAGGGGATGCTTTTTATAGGCAGTTTTATTTCTTCTCTTATTCTTCCTT2350.10616910253224605No Hit
ATCAATGGGATAGTTGCTCAGTGACAAAGCAAAGGAAGTTTAATATTTTCCA2340.10571731911721521No Hit
TAGAAAGGGCCTGAGAGAATAACAAATCTGTGATTTTATTATGTCTTTTATC2340.10571731911721521No Hit
TTGCGTGGGGAAAGGATATGAGAAAGGTAATATTGGTAAATAAAGAAATAGG2330.10526553570218437No Hit
CTCTCGGGGGGAGCAAAAATGACAAGTTTTACTAGCTAAGTAGAGAAATAAA2320.10481375228715353No Hit
TCAACAGGGATAAGAAAAAGTGAAACAATTCTATGATTTTGAGAGAAAGTCA2320.10481375228715353No Hit
AATCAGGGTCGCTTTGTGTGGTCTGATAAATACATTGATATTTAGGTTGAAA2310.10436196887212269No Hit
TAGAGTGGGCTTCCGATCTTTGTGTGGGCCTGAAAAGCAAAACTGAAGGAGT2300.10391018545709188No Hit
TGCTATGGGGAGCCTGACCAACATGGAGAAACCCCGTCTCTACTAAAAATAC2290.10345840204206104No Hit
GGAACGGGGGACTTAAGAATGTATTTTCAGCCCAAGGAACAATTTTCCTGTT2290.10345840204206104No Hit
CGCTCTGGGTAAGAACTGTGCGGTCTCCAGAAAGTTCTAAATGTCATTGGCC2280.1030066186270302No Hit
AGCAATGGGATGAGATTCAGTTTTATTTTGAGGAATACCTGGAAGAACATAC2280.1030066186270302No Hit
GCAAATGGGGAATGATCTTCAGTGCTTTAGGGTTAGTTTCATACTTGGCTCA2280.1030066186270302No Hit
CACGTAGGGAGGAACTGTGAAGTTAACTGTCTCATGGGAGATTTCTGTTAAA2280.1030066186270302No Hit
GTGGCTGGGGGACTCCGCCCAGCGAGGCGGAGCCTAGGGAAGCCGCGGGCGG2270.10255483521199936No Hit
AATCACGGGACCCGCCACCACCACCACTACGCACAAATACCAATCTTCCAGT2270.10255483521199936No Hit
GCGTTAGGGACATTAAAATAAGTACATGTGTTGATACACACATACCACATAA2260.10210305179696855No Hit
AACAGTGGGCTCATAAATGTAACACATTAAAAAGAAAACTTTTTTGTAGCCT2260.10210305179696855No Hit
GCTACTGGGAATGTTCCAAAATCAATAAATACCAAGAGGTCAAACTGAAAGA2250.1016512683819377No Hit
GCTTCTGGGAGGCGTGAGCCACTGCACCTAGCGATTTTTTTCCTTATTCTCA2250.1016512683819377No Hit
TTTTGTGGGACTTCACCTCTGAGCCTCCGATGCTCTGTGAAATGGAAGGAAT2250.1016512683819377No Hit
ACTACTGGTTGCAGCAGTAGAGGTTTCTTAGGTAATTGCCAATGTATGTCGA2250.1016512683819377No Hit
AAACTAGGGTCAAGTGGACAAGTGATTTGAAATCAGCCATAGTGATTTACTT2250.1016512683819377No Hit
AGCCGAGGGATATCAGGCTTATCAAAAGTAGCTGTGCTCAAACATCCTGAAG2250.1016512683819377No Hit
AATTGGGGGATCTGTAGAGCTAAAAATTGATGCTTCTGATGGTAATGCTTAC2240.10119948496690687No Hit
GGGATGCTAGGGTAATTTAAAAACACAAAAAAAATTTTAGCTCTTCCCACTT2240.10119948496690687No Hit
GTGAATGGGCGACTTGCTTTTGTATCAGGACGAGGAGGCGCTGAACGACTCC2240.10119948496690687No Hit
GCTATGGGGGGTACGACTGGAGTACCGGCAGCGCTGGGATGAAGCCTTTCGC2230.10074770155187603No Hit
AGTGTCGGGGCTAGCAATGAAGTCCAGAAGTTGTCAATGGTTCATCCCCAGA2230.10074770155187603No Hit
AGCTACGGGGATTTCAGACATCTGGCCTCCAGGATTGTGAGGGAATAAATTT2230.10074770155187603No Hit
GAGTATGGGCTATTGAGATAATCATGTGGTTTTTGTCGTTGGTTCTGTTTAT2230.10074770155187603No Hit
GATTGCGGGAAAGAGAGGACAATTATGGGGAGGATATCAAAACAGAGGTTCC2230.10074770155187603No Hit
AAAACTGGGAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT2220.1002959181368452No Hit
TGTGGTGGGTCACTCAGCCTCAGTTTTCTTGTTTGTAAAATGAGATAATAAT2220.1002959181368452No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)