FastQCFastQC Report
Sat 31 Dec 2022
SRR5847268.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847268.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146906
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT6560.4465440485752794RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC4920.33490803643145955No Hit
CATGGCGGGCGAGGCGGGATTCCATGATATTTGGTCTCTAGGATGGCAGATT1770.12048520822839094No Hit
GGGCCGGGGAGGTTAAAAAAATGGCAGTGATGAATTTTAAGGGTTTCCCCCA1570.1068710604059739No Hit
CTGCAAAGGGGCTGCTGCGCCGTGCGAAGGAGGAGGAGATGAAGAGGTTCTG1560.10619035301485305No Hit
TTACTGGGGATTCATCAGTGAGATTAACTTAAAATTTTCCTTTCTTATAATA1540.10482893823261133No Hit
GGAGCTGGGGTCGCGACCGGATGACGGCTTCGCCGCGCCTGCCGATCCGCAG1540.10482893823261133No Hit
TGAATCGGGCTGAAGATGCAAAGCATCTTTTCATGTGCTTTTTGGCCATTTT1510.10278681605924878No Hit
CTTTATGGGAATTCTCATCATGGAGAAACCTTTATTCCTATCTCCCTTTCCT1490.10142540127700705No Hit
GTTATTGGGGTCCGTGAGTGCTGTAAATGCATTGATGGAAAAAAAGTGGGTA1480.10074469388588621No Hit
GCAACAGGGATCCTATTAAAATATTATGAATTTTACTGTGTTCTTTCATTTA1480.10074469388588621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)