FastQCFastQC Report
Sat 31 Dec 2022
SRR5847267.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847267.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146336
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT5850.39976492455718343RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC4730.32322873387273127No Hit
AGCTTCGGGTGGAGTTTCTCTCTCTCTTTAGATCAAATAATACTTTGCTTTT1640.11207085064509074No Hit
GCAACAGGGATCCTATTAAAATATTATGAATTTTACTGTGTTCTTTCATTTA1590.10865405641810627No Hit
GGGCCGGGGAGGTTAAAAAAATGGCAGTGATGAATTTTAAGGGTTTCCCCCA1580.10797069757270938No Hit
GCTACAGGGGCGATCTGTTGCCGGGCCTTTCTTTGAATAAAATAATTTCTCA1580.10797069757270938No Hit
CATGGCGGGCGAGGCGGGATTCCATGATATTTGGTCTCTAGGATGGCAGATT1560.1066039798819156No Hit
CGATTCGGGATTCTCCCTGAGATGATGTCTTCAGTAATGATCGCACGTATTG1520.10387054450032801No Hit
TAACAAGGGATTCAAGAGGAAGCAGAGCAGAAAACTTTGGAAAATTTGCAGC1490.10182046796413734No Hit
CGTGCCGGGATAACAATAGCTTCATAATATGTCTTTGATGTTTTGTATAGCA1470.10045375027334354No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)