FastQCFastQC Report
Sat 31 Dec 2022
SRR5847266.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847266.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146481
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT5360.3659177640786177RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC4130.28194782941132296No Hit
GCAACAGGGATCCTATTAAAATATTATGAATTTTACTGTGTTCTTTCATTTA1640.1119599128897263No Hit
TTACTGGGGATTCATCAGTGAGATTAACTTAAAATTTTCCTTTCTTATAATA1570.10718113612004287No Hit
AGCTTCGGGTGGAGTTTCTCTCTCTCTTTAGATCAAATAATACTTTGCTTTT1540.10513308893303568No Hit
TGAATCGGGCTGAAGATGCAAAGCATCTTTTCATGTGCTTTTTGGCCATTTT1530.10445040653736662No Hit
CGATTCGGGATTCTCCCTGAGATGATGTCTTCAGTAATGATCGCACGTATTG1510.10308504174602849No Hit
GACCTGGGTTTACAGACATGAGCCACCACACCTGGCCGACATTCTGATTTTG1500.10240235935035943No Hit
AAGAGGGGATTAGGTCAGCATGTGAAATGTTGGATTTGACATGTCAGGTAGG1490.10171967695469038No Hit
CAACATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1470.10035431216335225No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)