FastQCFastQC Report
Sat 31 Dec 2022
SRR5847262.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847262.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246178
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT8010.3253743226445905RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC7300.296533402659864No Hit
TCAACGGGGACAAAGATAAGCAAATGATTGCAGAAGATCTAGATCACAAAGG2910.11820715092331566No Hit
AAGTTCGGGTCTTTCTTTTTCTGTCTCTCTTGCTTTGTTCTTTATTTGTTTA2870.11658231036079585No Hit
ACTTTTGGGCACCTGCCTCCACCAGCTGCCAAGACTGTGGTTGAGAACCTCC2860.11617610022016589No Hit
CGTTGCGGGAACCTAATTGTATTGTAATTATAAGTTAACTGTTAGAAACAAA2820.11455125965764609No Hit
GCAGAGGGGCACGACGCTCTTTCGATCTGTAGCCGGGAGAAACCCTATGAAT2780.11292641909512628No Hit
TGTCTGGGGTCCCAGTAAAAGGCACTTATCGAAGCTGATAACCGTCCTTCAT2720.11048915825134659No Hit
GCCCGCCTCGGCCTGTAATCCCTTTGGGATTCCAAGGTGGTGGGATTACAGG2720.11048915825134659No Hit
TGTTCCGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG2700.10967673797008669No Hit
GCAACTGGGAGCAAGCAAGTTTGAGACAATCGCAGTTTAAAAGCATGAACCA2670.10845810754819683No Hit
ACTAAGGGGGGAGTATGAGGCCAAAAAGACTTTTTTAAATGTGCCAATAGCA2660.10805189740756688No Hit
TGCATGAGGGAACACAAAAGCTACGGATGGAGGAGCGATTTAAAAAGAAAGA2650.10764568726693692No Hit
TGTCAAGGGGCGCCGAGTGGGACAGCGCTGGTGCGGAGACTGCTTCCGGACT2640.10723947712630699No Hit
TCTTGGGGGCATCCATCAACTAACCAATACTGGAGTAACCGCTTCCTAAACC2620.10642705684504707No Hit
ACAAGCGGGTGAGGCAGGTGCCATTATTACTTCTACTCACAGATGAGAAAAT2610.10602084670441714No Hit
GCATTTGGGAAGCAATGTTTCATTTTCCTATTGTCTGTTTAAAAATATCATT2600.10561463656378718No Hit
AATGACGGGATGGCTCTTTTGGCCCATCCCCTGTGAGTGGTGGAGAATGCTC2590.10520842642315721No Hit
AGCTGAGGGAGGACATAGACGTGGGCAAGGACTTCATGTCCAAAACACCAAA2540.10317737572000747No Hit
TCGTCGGGGAGGCCACTGCCGATTCCACCAAGATGGACTGTGTGTGGAGCAA2540.10317737572000747No Hit
ACCTAGGGACAGCTTCTGGAGGAACAGAATTTGTCCCTTCTAGATCAACTGA2530.10277116557937752No Hit
CCTTCAGGGATAAATGATTTGCCAAAGGGATGCTCTTTAGAATGTGCTAATA2490.10114632501685772No Hit
TTTTCTGGGGCTTACGCAAAAGGTCACCATTTGAGGTCCTGCCTTACTAATT2470.1003339047355978No Hit
CCATTAGGGACGAGAAGGCGACGATGACCGGACTGTGTGCCGGGAGATCCGC2470.1003339047355978No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)