FastQCFastQC Report
Sat 31 Dec 2022
SRR5847261.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847261.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245948
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT7590.30860181827052874RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC7070.2874591377039049No Hit
TGACACGGGGCAGGTGCATTTCACAGAGAGGTCATTTGATCTTTTGGTAAAG2830.11506497308374128No Hit
GCAACTGGGAGCAAGCAAGTTTGAGACAATCGCAGTTTAAAAGCATGAACCA2830.11506497308374128No Hit
TGTTCCGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG2810.11425179306194806No Hit
TCAACGGGGACAAAGATAAGCAAATGATTGCAGAAGATCTAGATCACAAAGG2790.11343861304015483No Hit
AGTTACGGGATGAAAATGATACCTAATATTTCCAACTGAGCCAAAAACAATT2780.1130320230292582No Hit
TGCATGAGGGAACACAAAAGCTACGGATGGAGGAGCGATTTAAAAAGAAAGA2740.11140566298567178No Hit
TCTGACGGGTCTCAAAATTAAAATCTGGTCACATGAGCTTTAGTTTGTTTTC2720.11059248296387855No Hit
ACCTAGGGACAGCTTCTGGAGGAACAGAATTTGTCCCTTCTAGATCAACTGA2660.10815294289849887No Hit
AACAGGGGGCTTCCTGAGGGGTACAAGCTGTATCCTAATGCCCGAAGAGCTC2660.10815294289849887No Hit
CGTTGCGGGAACCTAATTGTATTGTAATTATAAGTTAACTGTTAGAAACAAA2630.10693317286580904No Hit
TTTTCTGGGGCTTACGCAAAAGGTCACCATTTGAGGTCCTGCCTTACTAATT2630.10693317286580904No Hit
ACTTTTGGGCACCTGCCTCCACCAGCTGCCAAGACTGTGGTTGAGAACCTCC2620.10652658285491241No Hit
ACAAGCGGGTGAGGCAGGTGCCATTATTACTTCTACTCACAGATGAGAAAAT2590.1053068128222226No Hit
GCTAGGGGGCTCCGGCGCGCGCTTCCTGTGAGGTCAGGTGGGAGGAAACCGC2580.10490022281132597No Hit
TTAAGAGGGGCTGTATCCAATGCTCAGGTGAGAGGTGATAGCTATGAAAGCG2560.10408704278953274No Hit
TGTCTGGGGTCCCAGTAAAAGGCACTTATCGAAGCTGATAACCGTCCTTCAT2560.10408704278953274No Hit
AGTGTTGGGATTACAGCAAATTTATTTACTCACAAAAATTGCTTTTCCAAGG2560.10408704278953274No Hit
AAACCAGGGGTTATGGATACATTTCAGAATCTAAGAAATCAGGCAAGTGCTT2550.10368045277863613No Hit
AAGTTCGGGTCTTTCTTTTTCTGTCTCTCTTGCTTTGTTCTTTATTTGTTTA2550.10368045277863613No Hit
TAAGTTCGGGCCAGGTGGATTCTGGTCCTAACTAAAGAGATGGGAGTTCACC2540.10327386276773952No Hit
AGTTCAGGGAATCCAAGATACTCTCTTATTTATAATAGAATACTTACTTCTT2520.10246068274594629No Hit
TGTCAAGGGGCGCCGAGTGGGACAGCGCTGGTGCGGAGACTGCTTCCGGACT2510.10205409273504969No Hit
TAACCTGGGAACCTCAAGCGATCCGCCCTCCTCGGCCTCCCATAGTGCCGGG2510.10205409273504969No Hit
AAATTTGGGATTCTCTGCTCTATAGTTGTGAAATGAAGAGTGAAAACAAATT2500.10164750272415309No Hit
AGCTCAGGGAAGGGACTGTGGAACCATATTTGTGTCCCAGTTGAAAAGCGAC2490.10124091271325646No Hit
CGTCAAGGGATTGATGGTGATGGTCAAGTAAACTATGAAGAGTTTGTACAAA2480.10083432270235985No Hit
GCAGAGGGGCACGACGCTCTTTCGATCTGTAGCCGGGAGAAACCCTATGAAT2480.10083432270235985No Hit
CTCTAAGGGAGAATCATGTTATTTAATTTCCATTTATTTGTGCTGCTTTAAA2480.10083432270235985No Hit
GTTAGCGGGATCTAACCTGTTCTTGATTTTATCAAGGAGGAAACTTCAGTCC2470.10042773269146324No Hit
CTTGTCGGGACTTGGAGGGAAGCCTGGAGGAAGCCAAGCAGTTATTTGGACG2470.10042773269146324No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)