FastQCFastQC Report
Sat 31 Dec 2022
SRR5847260.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847260.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243727
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC7930.3253640343499079No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT7890.3237228538487734RNA PCR Primer, Index 23 (95% over 21bp)
AGTTTTGGGGGCGCCTGCCTGATCCTCCAAATCACCACAGGACTATTCCTAG2850.11693411070583071No Hit
CTTGTCGGGACTTGGAGGGAAGCCTGGAGGAAGCCAAGCAGTTATTTGGACG2680.10995909357600922No Hit
ACAAGCGGGTGAGGCAGGTGCCATTATTACTTCTACTCACAGATGAGAAAAT2670.10954879845072561No Hit
TGCATGAGGGAACACAAAAGCTACGGATGGAGGAGCGATTTAAAAAGAAAGA2660.10913850332544199No Hit
TCGACAGGGGTGGCCGAAGGATGCCCGTTTGTGTCTAAACGGAGGCTCGGCC2630.10790761794959114No Hit
GCAACTGGGAGCAAGCAAGTTTGAGACAATCGCAGTTTAAAAGCATGAACCA2610.10708702769902391No Hit
CGTTGCGGGAACCTAATTGTATTGTAATTATAAGTTAACTGTTAGAAACAAA2600.10667673257374029No Hit
AGTTACGGGATGAAAATGATACCTAATATTTCCAACTGAGCCAAAAACAATT2590.10626643744845667No Hit
AATGACGGGATGGCTCTTTTGGCCCATCCCCTGTGAGTGGTGGAGAATGCTC2580.10585614232317307No Hit
ACTTTTGGGCACCTGCCTCCACCAGCTGCCAAGACTGTGGTTGAGAACCTCC2570.10544584719788944No Hit
AGTGTTGGGATTACAGCAAATTTATTTACTCACAAAAATTGCTTTTCCAAGG2570.10544584719788944No Hit
TCAACGGGGACAAAGATAAGCAAATGATTGCAGAAGATCTAGATCACAAAGG2540.1042149618220386No Hit
GCAGAGGGGCACGACGCTCTTTCGATCTGTAGCCGGGAGAAACCCTATGAAT2520.10339437157147137No Hit
TGTCAAGGGGCGCCGAGTGGGACAGCGCTGGTGCGGAGACTGCTTCCGGACT2520.10339437157147137No Hit
AATGGCGGGTAGTCATGGACAAAAGGTATGAAATACCTTTGTGGTGTTGAAA2510.10298407644618775No Hit
CAAATAGGGATGAAAAAGAGCAGCACTTTTGTTGTAGTTGTTGTTGTTAATA2510.10298407644618775No Hit
ACTAAGGGGGGAGTATGAGGCCAAAAAGACTTTTTTAAATGTGCCAATAGCA2480.1017531910703369No Hit
TCGTCGGGGAGGCCACTGCCGATTCCACCAAGATGGACTGTGTGTGGAGCAA2480.1017531910703369No Hit
TTCAGGGGATAGTCCAAGCTGCAGAGGGAAAGGATGTAACCTGATATTCATA2470.10134289594505327No Hit
GACAATGGGCTAACAAATGCTCTTTGCTGTCTCAGGCACTACATGTCATTGA2470.10134289594505327No Hit
TGTCTGGGGTCCCAGTAAAAGGCACTTATCGAAGCTGATAACCGTCCTTCAT2460.10093260081976967No Hit
AAGTTCGGGTCTTTCTTTTTCTGTCTCTCTTGCTTTGTTCTTTATTTGTTTA2460.10093260081976967No Hit
ATGCCTGGGCTGTTCTGTCTGCCATGGGTTTGTCCAGCTCAGGTAGCTCTAG2440.10011201056920242No Hit
TTAAGAGGGGCTGTATCCAATGCTCAGGTGAGAGGTGATAGCTATGAAAGCG2440.10011201056920242No Hit
TAAGTTCGGGCCAGGTGGATTCTGGTCCTAACTAAAGAGATGGGAGTTCACC2440.10011201056920242No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)