FastQCFastQC Report
Sat 31 Dec 2022
SRR5847256.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847256.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181530
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT6490.357516663912301RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC5960.32832038781468625No Hit
GACTTGGGGAGTAAAATAGGAACTGAAGATCTGTATCAAAATGTAACACTTT2320.12780256706880405No Hit
TACAACGGGGGCCTTCGAGGTGCCTTAGGCCGCTTGCCTTGCTCTCAGAATC2280.125599074533135No Hit
GTTACCGGGCATGGCTCAAGTTTTCCAGAGGTTCTCATTTCACTCAAAGAAA2190.12064121632787969No Hit
GCTCTCGGGATATAGAAAAATGTAGGCAAGGAGAAAAAAAAGTTTCTACCAG2160.11898859692612793No Hit
TTTGCTGGGAGAGTGAGGATGTTGATTTCAGATTTTTCTTAAATGTAGTTGT2120.11678510439045889No Hit
TTCCAGGGATAAAGCCTACTTGATCGTAGTGGATAAGCTTTTTGATGTGCTG2070.11403073872087259No Hit
TCTCACGGGGGTGACTACCTTATTATAAAATTCCAAGTTTCCAAGAGACTTC2060.11347986558695533No Hit
CTCCAGGGAAAGTAAACAGAGTCTTTGAATCTAGAAAGGGATCTTATTAAAA2050.11292899245303807No Hit
TAATGTGGGCAAAACAAAAATCCTTCAAAAGTTTCTATTGTGATAAGAAAAC2040.11237811931912081No Hit
GTATTGGGGAGAGGAAGTGGTTCTTGTGGGTGAAGGTCTGATCTTTTCAGAG2030.11182724618520355No Hit
GCTGTGTGTGCCTCTGTAGCTCCTCCTGACAAACGTCTGGGAAAACAGCCTC2030.11182724618520355No Hit
AAAGTCGGGGTGATTAAATGATGCTTCTCATATTCAGGTTGCAAACCGCTCC2000.11017462678345176No Hit
CGCACTGGGACTGCTGGGCGTGGAATGCCTCTCGGGCTCTGACAGTGTCATT1980.10907288051561724No Hit
ACCACCGGGATAGACATAATTACACAGAAGCTCAACTAAATTTGCAAACATT1970.10852200738169998No Hit
TGAATGGGGTAGGCAGCAGCTTGGGCTGGGAAGAGGTGGTGGCAGAGTCTCA1960.10797113424778272No Hit
GTAACTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1960.10797113424778272No Hit
CATAACGGGTCTCAAATATAAATCTGGTCATATTTGTTCCATGCTTACATCT1950.10742026111386546No Hit
TAATTAGGGCGATCTTCCGATGGGTATTACATTCAGAGAGAAGCAGTGGAGC1920.1057676417121137No Hit
ACAAAAGGGAACTTGTGTACAGTTTAGACAAGGAGAACAAAGAAATAATGCA1910.10521676857819644No Hit
ATAGGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1910.10521676857819644No Hit
CAACCCGGGGAGTTTGCAACATGAGTATCTCTAACCTTTTGCTTTTCAGGGA1900.10466589544427918No Hit
CTGTGGGGAGTCAAACTAGACTTTCATTTTTTCTATGTTAACGTGAATAAGT1900.10466589544427918No Hit
AGTTTGGGAGGCCTGTGAAGAAAAGCCTTTTCATCTTAGCTTTAATAAATAA1900.10466589544427918No Hit
TTTTCCGGGTCTGTCTGGTAGCAGTGAGACCCCTTGTCTTGGTGATCCTTAC1890.10411502231036192No Hit
GTGCCTGGGCGACAGAGTGAGACTCCATCTCCAAAAACGTGTTAGCTTTTAT1880.10356414917644466No Hit
ATAACAGGGCAACTCCTGACCTCAGGTGATTCACTTGCCTCAGTCTCCCAAA1880.10356414917644466No Hit
GCCTGGCTTGTTTCACTTGGTATGATGTTTTTCAGGTTCCTCTATGTTGTCA1870.1030132760425274No Hit
GGTCCAGGGCATTCAGGATATGGGTTTGCTATTGGGAACATGGCAGCCTTTG1870.1030132760425274No Hit
TCAAGTAGGAAGGCAAAACATTATGACATGATAATGAAGAACTACTTAAGAT1870.1030132760425274No Hit
GCATTAGGGGAGTGGCCTGGAATAAATGTAGACATGGAATAGGAACTTGCTA1870.1030132760425274No Hit
GAACATGGGCTATTATATGTGACTATTTTTGTAAATGGGTTTGTGTTTGGGG1860.10246240290861014No Hit
AAAGATGGGATAAGCTCTATACTTCAGTAATGTAAGTTTGAATTTCATCCTG1860.10246240290861014No Hit
GCTGACGGGATTTGGATATTCTTACTTTATAACTCAAGTATACAAGGCTCAG1850.10191152977469288No Hit
GACTTTGGGATGTAAGAGTGTTCAACTGTGAGTTTCATCATGTGTGTCCGTT1840.10136065664077562No Hit
GATTACCCCAGCTACTGAGACTGAAATGTGAACTGTATTGTTGAAGAGCTTT1840.10136065664077562No Hit
CTTTAAGGGCTGTAATGGATTTTGCATGGCCACCTTGCAGGTAGAATCAGAA1830.10080978350685836No Hit
TGTTAAGGGCTGAAGTATTTGGTACTAAATAATTAAATTAGCTAAGTGTCAA1830.10080978350685836No Hit
CTACGGGTATTCAACAAATATATACTGAGGTCCCTCTGTTCTGGGTGCTTGG1820.1002589103729411No Hit
GACTCAGGGGAGGAATAGATTGTTGTCTTTTATGTTCTGTATATAGTTCCCC1820.1002589103729411No Hit
CAGCTGGGGAGTCCTCGAAGGCTCGGAAGCCCGTCACCATGTCGTGCGAGTC1820.1002589103729411No Hit
TGCTATGGGGATATAACTGAAATTACACATTATATATTTTGACATAACTTGT1820.1002589103729411No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)