FastQCFastQC Report
Sat 31 Dec 2022
SRR5847255.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847255.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180972
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC6300.3481201511836085No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT6110.3376212894812457RNA PCR Primer, Index 23 (95% over 21bp)
GACTTGGGGAGTAAAATAGGAACTGAAGATCTGTATCAAAATGTAACACTTT2310.1276440554339898No Hit
CGCACTGGGACTGCTGGGCGTGGAATGCCTCTCGGGCTCTGACAGTGTCATT2270.12543376875980813No Hit
ATAGGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2260.12488119709126273No Hit
TAATTAGGGCGATCTTCCGATGGGTATTACATTCAGAGAGAAGCAGTGGAGC2260.12488119709126273No Hit
GGTCCAGGGCATTCAGGATATGGGTTTGCTATTGGGAACATGGCAGCCTTTG2200.12156576707999028No Hit
TTCTGTGGGTGGCTAAATAGCTTAAAAGAGAATGCATTAAATGATTGTTTTA2130.11769776540017239No Hit
CTGTATGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC2130.11769776540017239No Hit
TCGACAGGGCCGATCTCTGCCTGGGGATCTATGTAAGGGATACAAATGAATG2050.11327719205180913No Hit
TTCAATGGGGAAAAGACCATTTTCTCAGGTAAAAAAATCTATCATGCTTTGA2040.11272462038326371No Hit
GTAACTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2040.11272462038326371No Hit
GCCACTGGGAGCTCTGAGCAAATGGCTATGAATTCCCTTGAAGTTTTGTGTT2030.1121720487147183No Hit
AACTTGGGGTACCTTTGCACATGCCCTACTCTCCACTAATTATACTTAAAAG2030.1121720487147183No Hit
TTTGCTGGGAGAGTGAGGATGTTGATTTCAGATTTTTCTTAAATGTAGTTGT2030.1121720487147183No Hit
CTAAATGGGGTTCTAGTCAGAGTTGGTTCAATTAAAGGTGAAACTGTCTGTC2010.11106690537762748No Hit
TTCCAGGGATAAAGCCTACTTGATCGTAGTGGATAAGCTTTTTGATGTGCTG1990.10996176204053666No Hit
TAATTAGGGAGAGTCAGTCGAAGTTCCATCTGTCTTACCTTGATCATCAGAC1990.10996176204053666No Hit
CATAACGGGTCTCAAATATAAATCTGGTCATATTTGTTCCATGCTTACATCT1980.10940919037199125No Hit
GCAACAGGGGAGTTCTTTCCGGCTTCGCTGCTGGTGTCGGCTCCGAGAGCTC1970.10885661870344585No Hit
GGCATGGGGGTCTGAGGTAGGAGGATTGCTTGAGGCCGGGCCGGTCTTGAGT1940.10719890369780961No Hit
CTCCAGGGAAAGTAAACAGAGTCTTTGAATCTAGAAAGGGATCTTATTAAAA1930.1066463320292642No Hit
CTGTGGGGAGTCAAACTAGACTTTCATTTTTTCTATGTTAACGTGAATAAGT1920.10609376036071877No Hit
GTTACCGGGCATGGCTCAAGTTTTCCAGAGGTTCTCATTTCACTCAAAGAAA1920.10609376036071877No Hit
GGGCTTGGGTTAATGACTACATTTATGTTATTTTTCCTAAAATATCAGGACC1910.10554118869217338No Hit
TAATGTGGGCAAAACAAAAATCCTTCAAAAGTTTCTATTGTGATAAGAAAAC1910.10554118869217338No Hit
GATGTTGGGGAAGAACTTTGTGAAACTCCACAATCAAATGGGCCTGGGAAGG1910.10554118869217338No Hit
GTATTGGGGAGAGGAAGTGGTTCTTGTGGGTGAAGGTCTGATCTTTTCAGAG1900.10498861702362795No Hit
TGTGTAGGGAGGAAGAATTAGTGGAAGGTGTTATACAACCAGAAAGTATCAC1890.10443604535508257No Hit
GATTACCCCAGCTACTGAGACTGAAATGTGAACTGTATTGTTGAAGAGCTTT1880.10388347368653714No Hit
ATTAAAGGGCTCTGTAAGAATGCTTAGGCCTATAGTAAAATTGGGAAGATCA1870.10333090201799172No Hit
AAAGTCGGGGTGATTAAATGATGCTTCTCATATTCAGGTTGCAAACCGCTCC1870.10333090201799172No Hit
TCTCACGGGGGTGACTACCTTATTATAAAATTCCAAGTTTCCAAGAGACTTC1860.10277833034944633No Hit
TCTAACGGGATAATCAGCTTCAGAGATTCCTTAAACCTTCCTAATTTCCTGC1860.10277833034944633No Hit
AGGTATGGGATACCAAATACCTTAAATTCGGAACGGCAACATATTACATCAT1860.10277833034944633No Hit
AAACCAGGGATGCGGTTAATGAAGTTGTGGCAGGAATAAAAGAATATTTCAA1860.10277833034944633No Hit
TTAGTTGGGTAGAGATTGGGACTTACCCTTTAGGTTTTAGGAGGCTTGAGAA1850.1022257586809009No Hit
TGTGGAGAGGGACTGCTTTGAAACCAGCTATTTTGTATTCATAGCATTCTCT1850.1022257586809009No Hit
GACTCAGGGGAGGAATAGATTGTTGTCTTTTATGTTCTGTATATAGTTCCCC1850.1022257586809009No Hit
GTGTCAGGGATTTGAGTTGTTGGCAGAATTTAATTTCTTGTGGTTGAAGGAT1840.10167318701235552No Hit
ACTAGCGGGCTGAAAGCATTTCTCTATCAGAATAACAACTTACGACATAGAC1830.10112061534381009No Hit
CTCAACGGGGATCCACCTGCCTCGGCCTCCCAAAGTGCTAGGATTACAGGCG1820.10056804367526467No Hit
CGAGTAACTGGGACAATGTGTATGCACCACAGGTATTTGGTTTTCTGTTCCT1820.10056804367526467No Hit
TCCTTTGGCCTTCCTCCTGCTGGCAGCCATCACCGTGCTGGCGGTCGTCTTC1820.10056804367526467No Hit
TACGCCGGGATTCATTAAAAAGTCCTGGAAACAGCTGTTCTAGGATCTTGTC1820.10056804367526467No Hit
GCCATGGGGGCTGTCCATCCACTGATTCTGGGGTGAGAAGCGATTTCTACCT1820.10056804367526467No Hit
TGGTATGGGTGAGGATGGATCAGTTCTGAAACAGTTTCTTTCTGAAACAGAG1810.10001547200671927No Hit
TGTGCTGGGACCAGAAATGTCCCAGTGATTTCTTTAGCAAGTAGAAGCAAAT1810.10001547200671927No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)