FastQCFastQC Report
Sat 31 Dec 2022
SRR5847254.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847254.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180152
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT6150.3413783915804432RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC6100.3386029575025534No Hit
TTTGCTGGGAGAGTGAGGATGTTGATTTCAGATTTTTCTTAAATGTAGTTGT2310.12822505439850793No Hit
CGCACTGGGACTGCTGGGCGTGGAATGCCTCTCGGGCTCTGACAGTGTCATT2150.11934366534926061No Hit
TTCAATGGGGAAAAGACCATTTTCTCAGGTAAAAAAATCTATCATGCTTTGA2120.11767840490252675No Hit
ATAGGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2110.1171233180869488No Hit
ATTAAAGGGCTCTGTAAGAATGCTTAGGCCTATAGTAAAATTGGGAAGATCA2090.11601314445579289No Hit
AATTAAGGGAATTCACTTCCCTCTGAAATTAAGTGGCAAAGTAATAGCATCC2060.11434788400905903No Hit
TAATTAGGGCGATCTTCCGATGGGTATTACATTCAGAGAGAAGCAGTGGAGC2040.11323771037790312No Hit
TAATGTGGGCAAAACAAAAATCCTTCAAAAGTTTCTATTGTGATAAGAAAAC2030.11268262356232514No Hit
GAACATGGGCTATTATATGTGACTATTTTTGTAAATGGGTTTGTGTTTGGGG2020.11212753674674719No Hit
CATAACGGGTCTCAAATATAAATCTGGTCATATTTGTTCCATGCTTACATCT1990.11046227630001333No Hit
CTCCAGGGAAAGTAAACAGAGTCTTTGAATCTAGAAAGGGATCTTATTAAAA1960.10879701585327944No Hit
GGTCCAGGGCATTCAGGATATGGGTTTGCTATTGGGAACATGGCAGCCTTTG1940.10768684222212353No Hit
GTATTGGGGAGAGGAAGTGGTTCTTGTGGGTGAAGGTCTGATCTTTTCAGAG1940.10768684222212353No Hit
TTAGTTGGGTAGAGATTGGGACTTACCCTTTAGGTTTTAGGAGGCTTGAGAA1940.10768684222212353No Hit
TTCCAGGGATAAAGCCTACTTGATCGTAGTGGATAAGCTTTTTGATGTGCTG1940.10768684222212353No Hit
TTGCCAGGGATATCTCAAAGTAAAAAGGAAAAAATAAAAAAAGGTCTTTGTT1930.10713175540654558No Hit
GATACTGGGATCAAAGGCAAATTTTGCAAAAATAACAAGGTCAGAAAACCTC1930.10713175540654558No Hit
TGAATGGGGTAGGCAGCAGCTTGGGCTGGGAAGAGGTGGTGGCAGAGTCTCA1910.10602158177538967No Hit
TAGTTAGGGGATTCTTTCCAATTCTGACATTCGTCAAAAGTTAGTAAATACT1910.10602158177538967No Hit
AAACCAGGGATGCGGTTAATGAAGTTGTGGCAGGAATAAAAGAATATTTCAA1910.10602158177538967No Hit
GCATTAGGGGAGTGGCCTGGAATAAATGTAGACATGGAATAGGAACTTGCTA1910.10602158177538967No Hit
GCAACAGGGGAGTTCTTTCCGGCTTCGCTGCTGGTGTCGGCTCCGAGAGCTC1900.10546649495981172No Hit
CGAGTAACTGGGACAATGTGTATGCACCACAGGTATTTGGTTTTCTGTTCCT1870.10380123451307784No Hit
CAACCCGGGGAGTTTGCAACATGAGTATCTCTAACCTTTTGCTTTTCAGGGA1860.10324614769749989No Hit
TTTTCCGGGTCTGTCTGGTAGCAGTGAGACCCCTTGTCTTGGTGATCCTTAC1860.10324614769749989No Hit
ACAAAAGGGAACTTGTGTACAGTTTAGACAAGGAGAACAAAGAAATAATGCA1840.10213597406634398No Hit
GCCACTGGGAGCTCTGAGCAAATGGCTATGAATTCCCTTGAAGTTTTGTGTT1840.10213597406634398No Hit
TCTACAACTGGGGAAGCATAATTTGAGTAATTTGCCAGTGAAAATTAACTCT1840.10213597406634398No Hit
AAAGATGGGATAAGCTCTATACTTCAGTAATGTAAGTTTGAATTTCATCCTG1840.10213597406634398No Hit
CTTTAAGGGCTGTAATGGATTTTGCATGGCCACCTTGCAGGTAGAATCAGAA1840.10213597406634398No Hit
ATAACAGGGCAACTCCTGACCTCAGGTGATTCACTTGCCTCAGTCTCCCAAA1840.10213597406634398No Hit
AAAACGGGGCCCCATTTCTTCAACAAGTAAGTTGCAAGAAATAGTGAGGAAG1830.10158088725076603No Hit
ACTAGCGGGCTGAAAGCATTTCTCTATCAGAATAACAACTTACGACATAGAC1830.10158088725076603No Hit
AAAGTCGGGGTGATTAAATGATGCTTCTCATATTCAGGTTGCAAACCGCTCC1830.10158088725076603No Hit
TCTCACGGGGGTGACTACCTTATTATAAAATTCCAAGTTTCCAAGAGACTTC1820.10102580043518807No Hit
AGGTATGGGATACCAAATACCTTAAATTCGGAACGGCAACATATTACATCAT1820.10102580043518807No Hit
GTAAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1820.10102580043518807No Hit
GTTACCGGGCATGGCTCAAGTTTTCCAGAGGTTCTCATTTCACTCAAAGAAA1820.10102580043518807No Hit
GCTGACGGGATTTGGATATTCTTACTTTATAACTCAAGTATACAAGGCTCAG1810.10047071361961012No Hit
CAGTATGGGACCACCCACTACCCCGCACTTCCTGGGTCCCTGCAAAAATTGT1810.10047071361961012No Hit
AGTTTGGGAGGCCTGTGAAGAAAAGCCTTTTCATCTTAGCTTTAATAAATAA1810.10047071361961012No Hit
TGTGTAGGGAGGAAGAATTAGTGGAAGGTGTTATACAACCAGAAAGTATCAC1810.10047071361961012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)