FastQCFastQC Report
Sat 31 Dec 2022
SRR5847233.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847233.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206448
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTACTCGGATCTCGTATGCCGTCT19400.9397039448190344No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGGATCTCGTATGC19060.9232349066108657No Hit
ACTGCTGGGATTACAGACATGAGCCACTGTGCCTGGCCTTTTTCTTGCCCAT2680.12981477175850578No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGG2310.1118925831202046No Hit
CTCGCAGGGCTGATGTACAATTTTTTATCCCTCAACCCACTCCCACCTTTTC2310.1118925831202046No Hit
GCAAAGCGGGCACTTTTAGGTAGATAAGGCTACCGTCTCTATAGGTGATGGG2280.11043943269007207No Hit
GAAAAGGGTGAACAGACATTTCTCAAAAGAAGATGTATATGCGACCAACAAA2220.10753313182980703No Hit
CAATAGGGGGAGACAAAATGGGAATACAACAACGTGGGCACTGACCTGTCCC2160.10462683096954196No Hit
TTAGCGACGGGGAACACTGAGAGCTACAGATTTTCCTCTAAGCATCACACTA2150.10414244749283114No Hit
ACCGTTGGGTATACACTGAAACTACAAGTCGACTAAAGAATTACCAAGAGAA2140.10365806401612028No Hit
GCGCCTGGGAGATATTTTTTAAAGCAATATTGCTATTATTTTTGTCTTTGTG2110.10220491358598775No Hit
CGTGGCGGGCATCTTCAGAAGAACAGAGACCATTATAGCATCACTGAATTCC2080.10075176315585523No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)