FastQCFastQC Report
Sat 31 Dec 2022
SRR5847225.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847225.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125492
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTACTCGGATCTCGTATGCCGTCT20011.5945239537181652No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGGATCTCGTATGC19771.5753992286360885No Hit
CTATGACAGGGCCAATAAAGTCTGGGTTTTTAAATGTGCACCAACTTCTATT3320.2645586969687311No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGG2610.20798138526758678No Hit
GGAATAGGGGGAAGAATACCACTGGAATATTGATAGACATTTCACTGAATGT2350.18726293309533676No Hit
TTCGCGGGGGGTGGCCAGCCAGTACGGATGGTCTGGCAACATGGAGAGAATC2320.18487234246007714No Hit
TGAATAGGGTAGAGATGGGACTAGAGAAGTCACCTCAAGATTGGTGACAGAT2050.16335702674274058No Hit
AATGCTGGGTATGTAAGAGAGAATTCTTAATTTTTAAAATTTTTGACCTGAA2030.16176329965256747No Hit
TCCCACGGGAGCGCCTAAGAATGAGTTGGTCCAGAAGTTCCAAGTCTATTAC1980.1577789819271348No Hit
TAGTAAGGGTGCTGTCTTCATGACAGTGAGTGAGTTCTCATGAGATCTGGTC1960.15618525483696172No Hit
TTTACAGGGGGAGAAACACTGGACGTGTCTCTCTACAAAAGAGCTTTTACTG1940.15459152774678864No Hit
AAAGTAGGGTAAGATCACCATTGAATACATGGTCTGTGGTTGAACCAAACTT1920.15299780065661556No Hit
AGAGTGGGTATGCTAAATCAGCTAGTACTGAAACTGTTCAGATATGCAACAT1910.15220093711152902No Hit
TTGGTAGGGACTGAAGAAGCAGCATCTTTTCATGAAATGTGTTCTGTTGCTT1900.1514040735664425No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGGA1900.1514040735664425No Hit
TTTCATGGGGTGATAAATGAGCATTGACCTCAACTTAAAGTCACCAGCCCGT1890.15060721002135594No Hit
TACTTAGGGGGGGAGAAAGATGGCGGCAGCCGTGCGACAGGATTTGGCCCAG1890.15060721002135594No Hit
TACAAGGGGAGTCAGTGATTTGTGAGAACCCAAGCGATGATCAAAATATGAA1870.14901348293118286No Hit
ACACCCGGGCCTTGCTGGCATTGAATTAACACAGGGACAAAATTTGGTTAAT1860.14821661938609634No Hit
TTTATTGGGGGTTCCAGCAATGGAAAAGCAGCTTACACTGGGCCAAAAGAAC1850.1474197558410098No Hit
CATGGCGGGCGGGAAATGAGAAAGAAGAGGAGTGAGGAAACAGTTATTTCCT1820.14502916520575018No Hit
GAAGCAGGGAACCAGATTTTTAGATCTTGTGTGATTTCCAAGCCTTTTCAAT1790.14263857457049053No Hit
CACTACTGGGTATCTACCCTGAGGAAAATAAGTCATTATACAAAAAAGATAC1780.141841711025404No Hit
AATAATGGGGTCACCTGACTTCTTACTACAAGAACATCCTTCAGAAGTGAAA1780.141841711025404No Hit
ACTAACGGGCATGCACCAAGGCAGCTGTTGCAGCATTGTAATTGTTTTCTAT1780.141841711025404No Hit
TATGTTGGGGCCGGTTGACTTTGCGGAGCCATGGAGGGCGGCTTCGGCTCCG1770.14104484748031748No Hit
CTACGCGGGAGTCAGAGTTTAAATACATTTCATACTAAACATAGCAATACTC1750.1394511203901444No Hit
GAAACTGGGCATGAAAGGCTTTTCTGCAGCAGCGGCCATTGTCCTTTCCACC1750.1394511203901444No Hit
AAGAACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1750.1394511203901444No Hit
CAGGTGGGATTTGAGACAATGTTATCAAATATGGTAACTGAAGCTATTGAAG1750.1394511203901444No Hit
TTTGCAGGGATCTCTTAAATTACTCAAAAGAAACTTCCGAAAACGTAGCTGT1740.13865425684505783No Hit
GTGGGAGGACTTGATATTCATAATTCAGTGCTGTGGAAATGAAAAAAATGAT1740.13865425684505783No Hit
ACTTATGGGAGAGAGAGAGAGAGAGACTTGGAGAGGGAAGGAAGGGAAGGAG1730.13785739329997132No Hit
CGCACCGGGTGGGAACAGGAAAGCCAACAGAGAGGCAGTGAAGGAAAGTTCC1730.13785739329997132No Hit
CAAGCTGGGTAGGCAAAGACTAGAGTTGTAGCTTAAAATGTAGTTAATGTAG1690.13466993911962516No Hit
AGTATAGGGGTTGAACCACCCTTGCATCTCAGGGATAAATCCCACTTGGCCA1670.13307621202945208No Hit
GAATAAGGGGATCTTCGTCAGGGGCACTGGTGATAAACATCCCTCCAAGAGA1660.13227934848436554No Hit
CATATAGGGAACCTGTAGGATCTTATTTGGAATTGACATTCTCTATTGTAAT1660.13227934848436554No Hit
GATATGGGGGGCTGAAACAGTCAGACCTACACCTAGGAGATGAGATCATTCA1650.131482484939279No Hit
ACGAAGGGGATCCAGTTAATCACCAGTAATCATGATGCTTTATGAATGACAA1640.13068562139419246No Hit
GTATTTGGGGGAGGAGTTGCAGTGGAAAGTTAAATGAGGCTGGAAAGTTTTG1630.12988875784910592No Hit
AGCGATGGGTAGTATTAGTCTCCACTAAGAAAGGGACAGGGAGGTGTGCAGG1630.12988875784910592No Hit
TGTGCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1620.12909189430401938No Hit
TAAAGTGGGGAAAAAATTTTTGAGATTGGTTGTACAACAACGTGAATATATT1590.12670130366875976No Hit
GTAATGGGAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGC1590.12670130366875976No Hit
GCGACAGGGGCAAACAGAGAGCCTGTGGGAGGGGCTGACAGCAGCAGCCGGC1580.12590444012367322No Hit
AGCGCAGGGATGTTCTTTCAAGTGTTGGCCTTTTATTGTCTTGTATTTGTGT1580.12590444012367322No Hit
ACTATTGGGCTCCTGAGATCTTATTGGGAAGCTGCTGATCACCAGCGTCAGG1570.12510757657858668No Hit
CGCATTGGGACAATATATTCTGTAATGAAATGAGGAGTGAATCTGAATATTT1570.12510757657858668No Hit
CACTCTGGGGCTTAGCAGTATCCCTGGCTGGAAGTGCTCCTCCCACACCCCT1550.12351384948841361No Hit
TTAATTGGGAGAGCCAAGCACGGTGCCTGACATTTCTCCACTGGACAAGTGA1550.12351384948841361No Hit
CGCATGGGGCAGCAGAGTAAATAGACAACCCAAAGAGTGGAAGAAAATCTTC1550.12351384948841361No Hit
TGACCAGGGATGCTCTTTGATGTTACATAAAAATAAAGTTTTATCTTTGTAT1540.12271698594332706No Hit
GATTCGGGAGACTCGTGGTACTACCCGTCCTATCCTCCCTCCTACCCTGGCA1540.12271698594332706No Hit
TAAAGCGGGGTTTCTGTGTTAGTAGTCTGTCTTGACACACATTGTTCAAACA1530.12192012239824052No Hit
GTAAATGGGGTACTTTGTGGCAATAGAATTATTGTCATGAAACTCGATTTTT1530.12192012239824052No Hit
ATTATGGGAACTGTTCTCTGCCTAGCGGCATGGGATTTGTCTCTTTATGCAC1530.12192012239824052No Hit
GTTCAAGGGGGCTTTGAACATGGGGTTGGCTGTTTCCCAGTAAAACTGGAAT1530.12192012239824052No Hit
TCCTTCGGGGACGCTCTTCCGATCTGAAATAGGGATAGAAAAGGTATAGTAA1520.12112325885315399No Hit
ATTAATGGGTCAGACACATGAAATGTGGTGACACATCCAGCAACAAGTCAAA1510.12032639530806745No Hit
AGCTACGGGGGGATCTGAGGTACAAACTGGAACCCGGGTCTTCTGGGCTCAG1510.12032639530806745No Hit
GTTTTTGGGGAGTGGGTGTTGCAAGATTCTCGTTAAGTCCTTTACTCCTGTG1500.11952953176298091No Hit
TCCTTAGGGGGTTTATTTTAAATGCGTACATATTGACCAATGGCCTCTGAAA1500.11952953176298091No Hit
TTACGCAGGGGGCAGCGACATGAAGTCAGGTATGCTCTGGGTGCGTGCTCTA1490.11873266821789435No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGGATCTCGT1480.11793580467280784No Hit
GGCATAGGGGTCGACACTTCTTGACAGTTAATTTTTGGAAGCAGATTATCTC1480.11793580467280784No Hit
ATTGAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA1480.11793580467280784No Hit
AAACTAGGGGGTCCTGTGCGGTCACTTAGCCAAGATGCCTGAGGAAACCCAG1460.11634207758263475No Hit
GCCTCGGCCTCCCAAAGTGCTGAGATTACAGACATGAGCCACCACGCCCGGC1460.11634207758263475No Hit
AGCTCAGGGAATATCAGTCGCCCCATCGTCTCCTCCCATGGGTAGGAGGAAG1460.11634207758263475No Hit
TTGCTTGGGTCTAAAATGAATTCAGAGAAATAGATTCCTAATCAGAGAAGGT1460.11634207758263475No Hit
CCAAAAGGGGTGGATGGCAGTGTGGGGAGGGCAGGTCCAGCTCTGTGGGCCC1460.11634207758263475No Hit
CTATAGCGGGATAGAATACTTTAAAAATGCCCTATAATCAAGGATCCTAGGT1460.11634207758263475No Hit
CGAAATGGGGCATTGGGTTTTTTCAAAGGGGGAACACTTTAGTGAACAATAG1450.11554521403754821No Hit
CGAAGTGGGTCTACACGGGGGACCATTTTGAATGTGTATTTGAGAGAAAGCT1450.11554521403754821No Hit
AGTCAAGGGGGAGAAATGTCCTCTTGTTCTGTAATTTTCCTTTCTTGCTGGT1450.11554521403754821No Hit
TCAGTGGGGAAGGACTAAAGAGAAATCCAAAATCATCATCATTATCATCTTA1450.11554521403754821No Hit
TCAAGAGGGCTAACCTGTGCCCTGTGGTTTCTGGAAGGAAAGGCAGACTTTA1450.11554521403754821No Hit
GCTGGTGGGGAAGCTGCAGAACGACAAGGCTAAACTAGAAGTGAAATCTCAG1440.11474835049246168No Hit
TGACAGGGGCGATCTCTTAAGGGGCCTGGCGTTGGTGACATGCTGCAGCCTG1440.11474835049246168No Hit
CTCAAGGTGGGATTGTATGATTTTGCTCATTTTTAAAATGTATGTAAAAGGA1440.11474835049246168No Hit
TACTCGGGGATTACCGGATCTGGATTGGGGAGGTTGGTCAGGGAAGAGAGGG1430.11395148694737514No Hit
TCGCTAGGGTCGGCAGTTTAGTCATCCACCGCCATCCTGGGCCCCACGTGTT1430.11395148694737514No Hit
AGCTGTGGGACTATATCTGCATTATTGATATTTTTAAGTAGTAGTTTTAAAA1430.11395148694737514No Hit
AGCAGGGGAAAAGAGAAAATGGTGGAATTTTTATTGAAAAAGAAAGCAAGTA1420.11315462340228859No Hit
GACTGCGGGCTGTAGCACTTCTGTGCCTTAGTGATGCATGTCCTACCTCCAT1420.11315462340228859No Hit
AATCAGGGATTGTGGTTTTCTAGATTGTTTCCTCAAGCCTTGTAACCTCCTA1410.11235775985720205No Hit
CGTTAGGGGCAGCAGGTGGTTATCTACAAGCGAGGATGAGAGGTCTCACCAG1410.11235775985720205No Hit
CAACGCGGCGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1400.11156089631211552No Hit
ATTGTTGGGACATTTCTCTTCTTGGTGTTGTAATACTATATTTTGTCAATAT1400.11156089631211552No Hit
CACAGGGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1400.11156089631211552No Hit
CACGCTGGGATGAGAAGTCTGTACTCTCTGCTCCGTTTAGCTGTGAACCTGC1390.11076403276702898No Hit
CTGAAGGGGTCTAGAAAATAGAGTTGGAGAAGAGAATGAAGCTGATAATGTG1380.10996716922194244No Hit
CAATCTGGGAAGTAAAAATAGAGGTATATACCATATATTCATAAAACATGTA1380.10996716922194244No Hit
CGTTTTGGGCAGTGCCCGGCCAAGTATTTTTATATTTCAGATCATATCTACT1380.10996716922194244No Hit
ATCAGCGGGTTAATAGGGAATAAGATCACCACAGAAAAATGTGTTCACCTTC1380.10996716922194244No Hit
TGCAAAGGGAGCAAATCTGATCATACATTACTAAAATAGATTGCTGATTGTC1380.10996716922194244No Hit
AGATAGGGGAAGCAGAATGAAGGAAAAAATGAAGAGCAGAAATTAATGAAAT1370.10917030567685589No Hit
GCCGCTGGGGGTAGTAGAAAAATTGCTACCTCTAAGAATGGATCCTGGAGAG1360.10837344213176936No Hit
GCATTAGGGACACCACTTACTACCTTATGAATTCAGGCCACTTACTCAGTTT1360.10837344213176936No Hit
GGCTAGGGTGATCATTTTATGTTTGAGTAACTCAAAACATTTGTAGGCATCA1360.10837344213176936No Hit
CGCGCAGGACTAATACTGAGTGATTGCAGAGTGCTTTATAAATATCACCTTA1360.10837344213176936No Hit
GCTCTAGGGCTGGAGCTTCAGTGTCTCTTCCCTCCCTCTAATAGTAATGGTT1360.10837344213176936No Hit
TCAGCCGGGGCTCTTCTGGGGGTGGATTATAAATTTCTTTCATACGGTTGTA1360.10837344213176936No Hit
TTGCGCTGGGATCTTTGGTTAGAAACACAAAAAAATAAAACTAGTAATATTG1360.10837344213176936No Hit
TGTGAGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCC1350.10757657858668282No Hit
TTCATTGGGGTAACAGATGTCCAAAGAGTTGCCATTTTCTTAACACTAAATA1350.10757657858668282No Hit
TCGCACGGGTGCCTCACAGGACTTTATTTTATGGGCTTGTGTTCGAGCTACT1350.10757657858668282No Hit
TGTATGGGGCACTCGAGAATCGGTCTTGTACTTTTTTTTCCTCTGGATTCCT1340.10677971504159628No Hit
TATCCAGGGGTAGCGGCCGTGGAGGAGGTCGGCTCAGCCGGGCAGTTTGAGG1340.10677971504159628No Hit
AGGATTGGGTGCTCACTGGAGCATTTTGGATTTTTGGATTTGGGATGCTCAG1340.10677971504159628No Hit
CGAGACGGGGGGACCAGGTGAAGGCTATCAACTTCCTGCCAGTGGACTATGA1330.10598285149650974No Hit
ACTTGGGGAGCAAAAACAGTAATAGCTACAGCAGCCTGCTAAGAGGTAATAT1330.10598285149650974No Hit
CGCTAATCCGGGACCCCACATTACCCAATCAGTAAAACATTTAGGTGTTTGC1330.10598285149650974No Hit
GGTGCTGGATAGCAAGTCCTATAATTCTCATTAAACACAGGAACCCAGCACA1330.10598285149650974No Hit
TCAAGAGGGCTTTAAAGTGTTCAAAAGTATTACTAGCAAAGAGGAGGAGGAG1330.10598285149650974No Hit
TGATGCAGGGATAAGAAGGTTGAGAAGGTGACAATCTCCAATAGACTTGTGT1330.10598285149650974No Hit
TTGTGAAGGGCAATAGTGAAAGTAATTGTGCAATTTCAAATAGAGATTTAGA1330.10598285149650974No Hit
GCCTTAGGGCCCATGATCCAAACACCTCCCACCAGACCCCACCTCCAACACT1320.10518598795142318No Hit
CAAAGTGGAATGACTGGCATTTGAGATGCTGCTGGCATTTTGAATTCAACAT1320.10518598795142318No Hit
AGCTCCGGGGGGAGATGATTCCTATATAATATATGGCACTGGAGGAACTAGC1310.10438912440633666No Hit
AGCCATGGGTGTCCCAGAAACACATCTTCCACCTCAGCAGCCAGTGGGAAAA1310.10438912440633666No Hit
ATAGTAGGGAGGAGTTTTTCTACGAGATAGAGCTCTCCACTCTGGCCACCAA1310.10438912440633666No Hit
CTTCTAGGGACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGCTG1310.10438912440633666No Hit
TGGTATGGGATCAAGGGGGGTAAATATGGGGATCTACTTTCTAAACCTTAAA1310.10438912440633666No Hit
TGCTCACGGGATGTGAGAGTACTTAGTTGAAACAAAAAGGAATTTTAGTAGA1310.10438912440633666No Hit
TGTACTGGGGCCCCGGCACTGCGCACTTGCCAGCCAGTCCGCCCGTCCGGAG1300.10359226086125012No Hit
TGACCCGGGAGTGAGAGGTCTTGGAAGGATCCTTGCGCCTCTCCTCTTTGTT1300.10359226086125012No Hit
CGCAACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1290.10279539731616358No Hit
ACTAGTGGGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGT1290.10279539731616358No Hit
TAAGAGGGGGAGTCCCTGCAGACCAACGTGCAGCGGCTGAAGGAGTACCGCT1290.10279539731616358No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGGAACTCGTATGC1280.10199853377107704No Hit
GGTACGGGGATGACCCTGGCTCATAAACACATTGTCAAGACTGTGGATTTCA1280.10199853377107704No Hit
GGGGTGGGGATCTGGAAAAAAATTCATTTTAAATACCATCATTCAACAAATT1270.10120167022599051No Hit
GGATTTGGGTGGATGGTGTAGATTATGGAGTTGTACTCAGCAGCTGATTCCA1270.10120167022599051No Hit
ACTTCCGGGAGGAGCAGTAGGCCATACCATACAGCTTAGGTGTGTTGTAGGC1270.10120167022599051No Hit
TTACGGGGTATATGGTCTTGCAACAGTTTTAAAGCCTCTGCATAATTGATAA1270.10120167022599051No Hit
AGCGAGGGTAACTTTTTTGATAAAACAAGAACAGTAAAGAAATAGCCATAGT1260.10040480668090396No Hit
TGTAAGGGATATATATTTTCCTTTAAACTTTTTAAGGTCACTTTTAAATAAC1260.10040480668090396No Hit
TGCTGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1260.10040480668090396No Hit
TAGAGTGGGCCAGCAAGTGATCAGGAAGACTAGGAGCTATTTCTGACTGTAA1260.10040480668090396No Hit
AGATAGGGGGTGCGCGGGTTGGTTTGCCCATGGAGTTTTAATTAAAAAAATT1260.10040480668090396No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)