FastQCFastQC Report
Sat 31 Dec 2022
SRR5847224.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847224.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123276
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGGATCTCGTATGC18691.5161101917648203No Hit
CTTATACACATCTCCGAGCCCACGAGACGTACTCGGATCTCGTATGCCGTCT18641.5120542522469904No Hit
CTATGACAGGGCCAATAAAGTCTGGGTTTTTAAATGTGCACCAACTTCTATT2860.23199974041987087No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGG2740.22226548557707906No Hit
GGAATAGGGGGAAGAATACCACTGGAATATTGATAGACATTTCACTGAATGT2370.19225153314513774No Hit
TTCGCGGGGGGTGGCCAGCCAGTACGGATGGTCTGGCAACATGGAGAGAATC2080.16872708394172425No Hit
TTTCATGGGGTGATAAATGAGCATTGACCTCAACTTAAAGTCACCAGCCCGT2030.16467114442389436No Hit
AATGCTGGGTATGTAAGAGAGAATTCTTAATTTTTAAAATTTTTGACCTGAA1990.16142639280963042No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGGA1950.15818164119536648No Hit
TCCCACGGGAGCGCCTAAGAATGAGTTGGTCCAGAAGTTCCAAGTCTATTAC1950.15818164119536648No Hit
TACAAGGGGAGTCAGTGATTTGTGAGAACCCAAGCGATGATCAAAATATGAA1930.15655926538823453No Hit
TTGGTAGGGACTGAAGAAGCAGCATCTTTTCATGAAATGTGTTCTGTTGCTT1890.1533145137739706No Hit
TCCTTCGGGGACGCTCTTCCGATCTGAAATAGGGATAGAAAAGGTATAGTAA1820.14763619844900874No Hit
AGCAGGGGAAAAGAGAAAATGGTGGAATTTTTATTGAAAAAGAAAGCAAGTA1820.14763619844900874No Hit
GAAGCAGGGAACCAGATTTTTAGATCTTGTGTGATTTCCAAGCCTTTTCAAT1810.14682501054544275No Hit
AGCTACGGGGGGATCTGAGGTACAAACTGGAACCCGGGTCTTCTGGGCTCAG1810.14682501054544275No Hit
ACTAACGGGCATGCACCAAGGCAGCTGTTGCAGCATTGTAATTGTTTTCTAT1790.1452026347383108No Hit
TTTACAGGGGGAGAAACACTGGACGTGTCTCTCTACAAAAGAGCTTTTACTG1780.1443914468347448No Hit
CACTACTGGGTATCTACCCTGAGGAAAATAAGTCATTATACAAAAAAGATAC1750.14195788312404686No Hit
CGCATTGGGACAATATATTCTGTAATGAAATGAGGAGTGAATCTGAATATTT1740.14114669522048087No Hit
CTACGCGGGAGTCAGAGTTTAAATACATTTCATACTAAACATAGCAATACTC1720.1395243194133489No Hit
TTTATTGGGGGTTCCAGCAATGGAAAAGCAGCTTACACTGGGCCAAAAGAAC1720.1395243194133489No Hit
GTATTTGGGGGAGGAGTTGCAGTGGAAAGTTAAATGAGGCTGGAAAGTTTTG1700.13790194360621694No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGGAACTCGTATGC1690.13709075570265095No Hit
CTCAAGGTGGGATTGTATGATTTTGCTCATTTTTAAAATGTATGTAAAAGGA1690.13709075570265095No Hit
CATATAGGGAACCTGTAGGATCTTATTTGGAATTGACATTCTCTATTGTAAT1680.13627956779908498No Hit
TAAAGTGGGGAAAAAATTTTTGAGATTGGTTGTACAACAACGTGAATATATT1640.13303481618482105No Hit
TATGTTGGGGCCGGTTGACTTTGCGGAGCCATGGAGGGCGGCTTCGGCTCCG1640.13303481618482105No Hit
GAAACTGGGCATGAAAGGCTTTTCTGCAGCAGCGGCCATTGTCCTTTCCACC1630.13222362828125508No Hit
TTACGCAGGGGGCAGCGACATGAAGTCAGGTATGCTCTGGGTGCGTGCTCTA1630.13222362828125508No Hit
TGAATAGGGTAGAGATGGGACTAGAGAAGTCACCTCAAGATTGGTGACAGAT1620.1314124403776891No Hit
CGTTAGGGGCAGCAGGTGGTTATCTACAAGCGAGGATGAGAGGTCTCACCAG1610.1306012524741231No Hit
AAGAACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1610.1306012524741231No Hit
AGTATAGGGGTTGAACCACCCTTGCATCTCAGGGATAAATCCCACTTGGCCA1600.12979006457055714No Hit
GCATTAGGGACACCACTTACTACCTTATGAATTCAGGCCACTTACTCAGTTT1590.12897887666699115No Hit
CATGGCGGGCGGGAAATGAGAAAGAAGAGGAGTGAGGAAACAGTTATTTCCT1590.12897887666699115No Hit
GTAAATGGGGTACTTTGTGGCAATAGAATTATTGTCATGAAACTCGATTTTT1590.12897887666699115No Hit
AGTCAAGGGGGAGAAATGTCCTCTTGTTCTGTAATTTTCCTTTCTTGCTGGT1580.12816768876342516No Hit
AGCGATGGGTAGTATTAGTCTCCACTAAGAAAGGGACAGGGAGGTGTGCAGG1580.12816768876342516No Hit
TTAATTGGGAGAGCCAAGCACGGTGCCTGACATTTCTCCACTGGACAAGTGA1570.1273565008598592No Hit
GTTTTTGGGGAGTGGGTGTTGCAAGATTCTCGTTAAGTCCTTTACTCCTGTG1560.1265453129562932No Hit
TCCTTAGGGGGTTTATTTTAAATGCGTACATATTGACCAATGGCCTCTGAAA1560.1265453129562932No Hit
CAGGTGGGATTTGAGACAATGTTATCAAATATGGTAACTGAAGCTATTGAAG1560.1265453129562932No Hit
GCGACAGGGGCAAACAGAGAGCCTGTGGGAGGGGCTGACAGCAGCAGCCGGC1550.1257341250527272No Hit
GGCATAGGGGTCGACACTTCTTGACAGTTAATTTTTGGAAGCAGATTATCTC1540.12492293714916124No Hit
GTGGGAGGACTTGATATTCATAATTCAGTGCTGTGGAAATGAAAAAAATGAT1540.12492293714916124No Hit
AGAGTGGGTATGCTAAATCAGCTAGTACTGAAACTGTTCAGATATGCAACAT1540.12492293714916124No Hit
TAGTAAGGGTGCTGTCTTCATGACAGTGAGTGAGTTCTCATGAGATCTGGTC1530.12411174924559525No Hit
TTAAGTGGGTGGTATCAATCAGAGATCGAATTAAATATTATAGTTCATTAGG1520.12330056134202928No Hit
CGCAACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1520.12330056134202928No Hit
AAAGTAGGGTAAGATCACCATTGAATACATGGTCTGTGGTTGAACCAAACTT1510.12248937343846329No Hit
ATTGTTGGGACATTTCTCTTCTTGGTGTTGTAATACTATATTTTGTCAATAT1500.1216781855348973No Hit
CACGCTGGGATGAGAAGTCTGTACTCTCTGCTCCGTTTAGCTGTGAACCTGC1500.1216781855348973No Hit
ACTATTGGGCTCCTGAGATCTTATTGGGAAGCTGCTGATCACCAGCGTCAGG1490.12086699763133132No Hit
ACGAAGGGGATCCAGTTAATCACCAGTAATCATGATGCTTTATGAATGACAA1490.12086699763133132No Hit
ATTGAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA1490.12086699763133132No Hit
ACTTATGGGAGAGAGAGAGAGAGAGACTTGGAGAGGGAAGGAAGGGAAGGAG1490.12086699763133132No Hit
TCGGCCGGGGGGGCGGGACCTGCAACGTCCGACAGAACGAGGGGACGTAACG1490.12086699763133132No Hit
CTGAAGGGGTCTAGAAAATAGAGTTGGAGAAGAGAATGAAGCTGATAATGTG1480.12005580972776533No Hit
ACTTCCGGGAGGAGCAGTAGGCCATACCATACAGCTTAGGTGTGTTGTAGGC1480.12005580972776533No Hit
ATAATCGGGAAAGAGTTAATGACTTGACAATTTATAGCTTAGTAGATGAAAG1470.11924462182419934No Hit
AGCCATGGGTGTCCCAGAAACACATCTTCCACCTCAGCAGCCAGTGGGAAAA1470.11924462182419934No Hit
CCTGGGAGATTGAACATGAAGCCTGGACCTAAGAAATGGTTTGGTAGTGTTG1470.11924462182419934No Hit
CACTCTGGGGCTTAGCAGTATCCCTGGCTGGAAGTGCTCCTCCCACACCCCT1470.11924462182419934No Hit
CAATAAGGGATTGTATTACAGTTTGCCTCTTCCTTTAGATCTAATCATATTT1460.11843343392063338No Hit
GATATGGGGGGCTGAAACAGTCAGACCTACACCTAGGAGATGAGATCATTCA1450.11762224601706739No Hit
AGCGCAGGGATGTTCTTTCAAGTGTTGGCCTTTTATTGTCTTGTATTTGTGT1440.1168110581135014No Hit
TCAGCCGGGGCTCTTCTGGGGGTGGATTATAAATTTCTTTCATACGGTTGTA1440.1168110581135014No Hit
CTATAGCGGGATAGAATACTTTAAAAATGCCCTATAATCAAGGATCCTAGGT1440.1168110581135014No Hit
GGGGTGGGGATCTGGAAAAAAATTCATTTTAAATACCATCATTCAACAAATT1430.11599987020993544No Hit
GCCGCTGGGGGTAGTAGAAAAATTGCTACCTCTAAGAATGGATCCTGGAGAG1430.11599987020993544No Hit
TGCTCACGGGATGTGAGAGTACTTAGTTGAAACAAAAAGGAATTTTAGTAGA1430.11599987020993544No Hit
GGCTAGGGTGATCATTTTATGTTTGAGTAACTCAAAACATTTGTAGGCATCA1420.11518868230636944No Hit
AATAATGGGGTCACCTGACTTCTTACTACAAGAACATCCTTCAGAAGTGAAA1420.11518868230636944No Hit
TGACCAGGGATGCTCTTTGATGTTACATAAAAATAAAGTTTTATCTTTGTAT1410.11437749440280348No Hit
AGATAGGGGAAGCAGAATGAAGGAAAAAATGAAGAGCAGAAATTAATGAAAT1410.11437749440280348No Hit
GGTACGGGGATGACCCTGGCTCATAAACACATTGTCAAGACTGTGGATTTCA1410.11437749440280348No Hit
CGCATGGGGCAGCAGAGTAAATAGACAACCCAAAGAGTGGAAGAAAATCTTC1410.11437749440280348No Hit
CACCTGGGGATCCAGAAACAGACTTCCTTTGCCATCTTGGTAAATTTTAATC1410.11437749440280348No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGGATCTCGT1400.11356630649923749No Hit
GAATAAGGGGATCTTCGTCAGGGGCACTGGTGATAAACATCCCTCCAAGAGA1400.11356630649923749No Hit
TTTGCAGGGATCTCTTAAATTACTCAAAAGAAACTTCCGAAAACGTAGCTGT1390.1127551185956715No Hit
TCATCGGGGCTTTTCACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGC1390.1127551185956715No Hit
CGCTAATCCGGGACCCCACATTACCCAATCAGTAAAACATTTAGGTGTTTGC1390.1127551185956715No Hit
TACTTAGGGGGGGAGAAAGATGGCGGCAGCCGTGCGACAGGATTTGGCCCAG1380.11194393069210552No Hit
CCAAAAGGGGTGGATGGCAGTGTGGGGAGGGCAGGTCCAGCTCTGTGGGCCC1380.11194393069210552No Hit
ACACCCGGGCCTTGCTGGCATTGAATTAACACAGGGACAAAATTTGGTTAAT1370.11113274278853953No Hit
ACCATAGGGCCAGTCACATGCAACTATTTTAATTTAAATCCATCAAACTTAC1360.11032155488497354No Hit
GTAATGGGAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTTCAGAGC1360.11032155488497354No Hit
TTGCTTGGGTCTAAAATGAATTCAGAGAAATAGATTCCTAATCAGAGAAGGT1360.11032155488497354No Hit
GGTGCTGGATAGCAAGTCCTATAATTCTCATTAAACACAGGAACCCAGCACA1360.11032155488497354No Hit
TCGCTAGGGTCGGCAGTTTAGTCATCCACCGCCATCCTGGGCCCCACGTGTT1360.11032155488497354No Hit
ATCAGGGGGATATCTAAAGGGGAATCATGATTATAAGTCCTTCAGCCCTTGG1340.10869917907784159No Hit
GCCTCGGCCTCCCAAAGTGCTGAGATTACAGACATGAGCCACCACGCCCGGC1340.10869917907784159No Hit
TGTGCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1340.10869917907784159No Hit
CAACGCGGCGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1340.10869917907784159No Hit
TGCAAAGGGAGCAAATCTGATCATACATTACTAAAATAGATTGCTGATTGTC1340.10869917907784159No Hit
AGAGTTGGGTTATAATCTGAACCTAGGTATATCCTTTGGTCTTCCACAGTCA1340.10869917907784159No Hit
AGCTCAGGGAATATCAGTCGCCCCATCGTCTCCTCCCATGGGTAGGAGGAAG1330.10788799117427562No Hit
GTTCAAGGGGGCTTTGAACATGGGGTTGGCTGTTTCCCAGTAAAACTGGAAT1330.10788799117427562No Hit
TCAAGAGGGCTTTAAAGTGTTCAAAAGTATTACTAGCAAAGAGGAGGAGGAG1320.10707680327070963No Hit
TAGAGTGGGCCAGCAAGTGATCAGGAAGACTAGGAGCTATTTCTGACTGTAA1320.10707680327070963No Hit
ATTATGGGAACTGTTCTCTGCCTAGCGGCATGGGATTTGTCTCTTTATGCAC1320.10707680327070963No Hit
TTACGGGGTATATGGTCTTGCAACAGTTTTAAAGCCTCTGCATAATTGATAA1320.10707680327070963No Hit
CACAGGGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1320.10707680327070963No Hit
TGGTATGGGATCAAGGGGGGTAAATATGGGGATCTACTTTCTAAACCTTAAA1310.10626561536714364No Hit
TAGGCCGGTCATTTATAAAAAGTACCTGTATCAAAGGAATTTTAACAAAGAG1310.10626561536714364No Hit
TTTCGCGGGGCAAAGAACATTGAATACAAACATTGGTCATTTTAGCCAAGTT1310.10626561536714364No Hit
CAAAGTGGAATGACTGGCATTTGAGATGCTGCTGGCATTTTGAATTCAACAT1310.10626561536714364No Hit
TCAAGAGGGCTAACCTGTGCCCTGTGGTTTCTGGAAGGAAAGGCAGACTTTA1310.10626561536714364No Hit
CAAGCTGGGTAGGCAAAGACTAGAGTTGTAGCTTAAAATGTAGTTAATGTAG1310.10626561536714364No Hit
TAAAGCGGGGTTTCTGTGTTAGTAGTCTGTCTTGACACACATTGTTCAAACA1310.10626561536714364No Hit
TATGCCGGGGCTTTTTTTCTTTGCTGCGTCTACTGCGAGAATGAAGACTATT1300.10545442746357767No Hit
CAAGTTGGGTACCAAGAAAAAATTAGTCACCTGAGTAACTTGGCCACTAATA1290.10464323956001167No Hit
TTCACCGGGGTATATTGAGAATCCCTTAAAATTACCAGACAAAAAAATTTAA1280.1038320516564457No Hit
AAGACTGGGGAACCCGAAACAAAAGTATCCTCAACTTAAGGTTTCCTTAGGG1280.1038320516564457No Hit
GCAGGTATCACAATTCCCTGTCCGTCAGAAAATTCATCAAGCCTGAGGAAAA1280.1038320516564457No Hit
GACTGCGGGCTGTAGCACTTCTGTGCCTTAGTGATGCATGTCCTACCTCCAT1270.10302086375287972No Hit
TTAATAGGGGAGTGGTGCTGGGAGTGTCGTGGACGCCGTGCCGTTACTCGTA1270.10302086375287972No Hit
TGAAACGGGGCGATCTGACGGCTGGGATTGGTGTTGATTTGAAAAAAGAAAA1270.10302086375287972No Hit
AATAAAGGGAGATATGGAGTTTCACCACGTTGGCCAAGACAGTCTCGATTTC1270.10302086375287972No Hit
CGCACCGGGTGGGAACAGGAAAGCCAACAGAGAGGCAGTGAAGGAAAGTTCC1270.10302086375287972No Hit
GCTGGTGGGGAAGCTGCAGAACGACAAGGCTAAACTAGAAGTGAAATCTCAG1260.10220967584931373No Hit
TGACTAGGGTACATTCTTCTTTCTGGTCCCATCTTAAACGTCTTCTGTTGTG1260.10220967584931373No Hit
CACTTTGGGGGGCTGAAGGACATCAAAATCTTATTTTTCAGCATTCTCTAGT1260.10220967584931373No Hit
AGCTGTGGGACTATATCTGCATTATTGATATTTTTAAGTAGTAGTTTTAAAA1260.10220967584931373No Hit
GATTCGGGAGACTCGTGGTACTACCCGTCCTATCCTCCCTCCTACCCTGGCA1250.10139848794574774No Hit
TAGTAGGGGCCATACAGTTGACACCATCCTATTACAAGAAAAACCTAACAGT1250.10139848794574774No Hit
TGATGCAGGGATAAGAAGGTTGAGAAGGTGACAATCTCCAATAGACTTGTGT1250.10139848794574774No Hit
GTTCACGGGATTTTATATAATTCTTATACATTTGTACTATTGATTTCTTGTA1250.10139848794574774No Hit
TCGAGGGGGGTATATTGATGTTACACTTGGTCACTTGTAATAGTTTGTCCGT1250.10139848794574774No Hit
GCTGCCGGGATCCGGAATATTCATTGAAATATCTTAGTGCTTCATACAAATT1250.10139848794574774No Hit
CGAAGTGGGTCTACACGGGGGACCATTTTGAATGTGTATTTGAGAGAAAGCT1250.10139848794574774No Hit
AGCTCCGGGGGGAGATGATTCCTATATAATATATGGCACTGGAGGAACTAGC1240.10058730004218178No Hit
CGCGCAGGACTAATACTGAGTGATTGCAGAGTGCTTTATAAATATCACCTTA1240.10058730004218178No Hit
CACGCTGGGACTGTAAGTCTTTGGCATCAAGAAATTACACAAAGATTCATAA1240.10058730004218178No Hit
GCTGGTGGGCAGGAGGAGCATTTTTATGATAGCAATTGGCCACATTCTAAGC1240.10058730004218178No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)