FastQCFastQC Report
Sat 31 Dec 2022
SRR5847223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219723
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTACTCGGATCTCGTATGCCGTCT16960.7718809592077297No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGGATCTCGTATGC14300.6508194408414231No Hit
CTGCTTGGGATCTCCCAAGAATAAGGGCATTTTCCTACAAAACCACAATGCC3080.14017649495046033No Hit
GTCGATGGAGTTGAATAACTAGCACGAGGTCACTCACCTAAAAAGTGGTGAG3050.13881113948016366No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC2950.1342599545791747No Hit
AATAGCGGGAATAAATGAAGGCATTAACTTTTCTCTCCCATGTGAGTGAAAT2890.13152924363858132No Hit
TTAGCGGGGCTTCCGAACCCCCATCCCTACCCCTGGCTGAGCTGGGCTGATC2780.12652294024749344No Hit
GGCAAGGGATTCTACAGGCTACAATCTAATGCTAACAGTTTATTTGGTTGCT2750.12515758477719674No Hit
ATACGGGGGTCTGCAAAGCTCCTCATTCAGTTATTCTGTAAGTTGATCCAAA2720.12379222930690005No Hit
CTTAATGGGATAGATGTACAAGGAAATGCATTTTCTGCCTCCACCCCCTTTT2720.12379222930690005No Hit
TTTACTGGGTACCCCTGAAGAAGCACAGACTTCGGATGTACAAAACAAAGAC2710.12333711081680115No Hit
TCACACGGGTATTTTCTCCATTAGTAATAGTAATATTTTGATATCTTAATAG2710.12333711081680115No Hit
TAGTTCGGGGATGGGACCTTGTCACTTTGAGCTAAAACCAGGCAAAGCTCAC2710.12333711081680115No Hit
TTATACGGGGAGTCCAGATCAAAAATGGCGGTAGTTGGTGTGTCCTCGGTTT2690.12242687383660336No Hit
AAGTACGGGTAAAAGTAGAGGTTTTGTAGGGGTACAGATCAATAACTGTCAC2680.12197175534650445No Hit
CGACGGGTGTGTAGGTAATAAAGAATCTGTTCAAATATGCATCAGTGGTATG2660.12106151836630667No Hit
GCTGCGGTGACAAAGCACTGGGTTTATTATGTATTAGTGCTGTAGTTATACT2660.12106151836630667No Hit
GTTAAAGGGCTTGGAAATAATTTGCTATCAAACTGAAAAAAAATCTTGAATG2640.12015128138610888No Hit
TAGAGGGGGAAAGCAGTGTGCATATCTTGAGGGGTGATGAACACAAGCAAAC2640.12015128138610888No Hit
AAGCCTGGGCAACAAGAGTGAAACTCCGTCTCAAAAATGAAAATAAAATAAA2610.11878592591581219No Hit
ATTTCGGGGACTTTGTTTTCTTTTAACCTGCTAAGTGGCATTCGGGAAACTT2610.11878592591581219No Hit
GGTCGAAGGTGACCAGTGTCTCAGTTCTCTTAATTCTATCAACAGCTTTGTT2610.11878592591581219No Hit
CTATGTGGGCAAGTTAATAACCTAACATCACAACTAAAAGAACTAGAGAACC2600.1183308074257133No Hit
TCAAGAGGGGATGTGTTCACATAATGAAATTTACTGGTCAAGTCTATCTGTA2590.11787568893561438No Hit
TGCGCAGGGGGTTGCATAAATTTGCGCTCCGCCAGCCCGGAGCATTTAGGGG2580.11742057044551547No Hit
GTGCTTGGGATGAGATAGAGACAAACACTATGTATTTCTGATGCTTTCAGGT2540.1156000964851199No Hit
ACGCTTGGGTCTGGTTGCTTAAGCTACATGTTTGAGAGAAGTTTCTAACCCT2530.115144977995021No Hit
TAATAGGGATCGCCATAAAAGGCCTGATACTGCCAATCATATATTGGACAAA2520.1146898595049221No Hit
TAATACGGGTGGATATATGTATTTCCCATTGGTCTAGCGGATGTCTTAAAAG2520.1146898595049221No Hit
CGCACTGGGGGCGAGACGGCTGGGCGCCGAGTGGGACAGCGCTGGTGCGGAG2510.11423474101482321No Hit
ACTTAAGGGATTTATTGATGTATAAAAATAGGATAATGGGGAACTGGACTGA2510.11423474101482321No Hit
AAGCGTGGGATAACTGACTGCCAAAACAAAGCCCAGCACTTTAAAAGAAAGA2510.11423474101482321No Hit
AAGCAGAGGGGGGGGTGGGATCTGAACATGGCGGCGGTGGTAGCTGCTACGG2490.1133245040346254No Hit
GCCGCAGGTACACATGGAAGATGAAGAATTACAACAATGAAGAAAAGTCCTC2490.1133245040346254No Hit
TTTACTGGGACCTGAATCATCTAAAAAACACGGTCTCAACCTGGCCACCGTG2480.11286938554452652No Hit
GTTTCAGGGAAGACAAAGCATATGGAGAAAATACTTCAAAGGGGAAGCAAAA2470.11241426705442761No Hit
AGCTGGGATTGAAATCCTTTTCAGAAAAAATATAATTGCAAATAGGTAGGAG2450.11150403007422983No Hit
GCAGTGGGGCCCAATTCAATAGGTGGAGCACAAAGAAGAATCATTTGATTTC2450.11150403007422983No Hit
ATTAGAGGGTCTAGGGAAGATCAGGTACATTGCAGAATTCAAAGCATGGTAA2450.11150403007422983No Hit
TGCTATGGGGAAGAAACAAAATCTAGTAATTTGGGGACAGCTAAATCCCACA2430.11059379309403203No Hit
TATTAAGGGTATGTATATTATGTCATTTAACGCTCACAACCACTCCATGTGG2430.11059379309403203No Hit
GAAGTAGGGGTAATTGCTGATACTAGGAATTAAGGGATTTTTTAAAAGTAGC2420.11013867460393315No Hit
TCGATTGGGATTGAATAAGGTATTCTTGTCAGTTTAGAAATGGACTGGATAA2420.11013867460393315No Hit
CAGTAGGGATATTAAGCAATATTGACTTGAAATGGATCATTTCCTTTTTTTA2420.11013867460393315No Hit
TTCGGCGGGGCTGTCATATTATGTATTTGGCTTGTAACCTACTTTTTATCAT2410.10968355611383424No Hit
AAGCGTGGGAAGAAATGCACAGGCGTGCATGGCATGCACGTTCAGACAGCTG2400.10922843762373534No Hit
AAAGTCGGGCACGGTGCTGTCGGGCGGCACCACCATGTATCCGGGCATTGCC2400.10922843762373534No Hit
GACCATGGGGGAACAGAAACAGGCATTCCTCCACAGTGAGTGGCTCCCCCTA2380.10831820064353755No Hit
AACACAGGGGAAAGGGAAACACCCTCAATTAGGAAACATTCCAAGGGGAGAA2380.10831820064353755No Hit
TGTGCTGGGGAGCGCGGAGGCAGCCATGGTGCGGTTCAAGCACAGGTACCTG2370.10786308215343865No Hit
CCAGGCTGGGATTGCAGGCGTGAGCCACCGCACCCAGCCGCCAGTCACTACT2360.10740796366333977No Hit
AGTCTTGGGCTGTCTGTGGTAATTCTCAAAACAAAATTTCACTTCTGTTTTC2350.10695284517324086No Hit
TCAGCGGGAAGCCATGATATTAGTCTCAGTGCCATGCTCTAAATTGGCTGAG2340.10649772668314195No Hit
TAGTGAGGGATTTCCTTCTTATACATTAAAAATAGTGTGAAACATGATTGAA2320.10558748970294417No Hit
AATATTGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTG2320.10558748970294417No Hit
AATATGGGTTTGTACAGTCTTTTAGACCTATTCAAGTGATGCTCATGATCCT2320.10558748970294417No Hit
CGCTATGGGAACCAAGGGAGCATAACAATGACATGAGGGCTTGGAAAGGAAA2310.10513237121284526No Hit
GTCACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2300.10467725272274636No Hit
ACCTAATTCATCCCTGCATGCAGTGCTCTTTAAAAGGAAAATCAGGCATTAA2300.10467725272274636No Hit
TGTCAAGGGGCCGTATGCAGGTGGACCCCCAAAAGTACAAGGGCATATTTAA2290.10422213423264748No Hit
TTATGTGGGACTATTCCAATCAATAGAAAAAGAGGGAATCCTCCCTAACTCT2290.10422213423264748No Hit
GCTTCAGGGATGATTTCTATGCATATTTATGGAGTTTTTTTCCTATGAAACC2280.10376701574254857No Hit
TTGCGAGGGGTAGTCTCTGTAGCATCTCCCCAAAGTGTTAAACAGCTACATA2280.10376701574254857No Hit
TAAAGCGGGGTTTCTGCATGTCAGCGAGCGACACTGTCCCTGCCTCAGGTTG2270.10331189725244967No Hit
GCTTGTGGGATATGTGAATGTCAGAGCAAGGATGGAACGGATAAACTGCCTA2270.10331189725244967No Hit
AGAGCAGGGCTCTGTTCCAATCTGCGTGGTGCTTCTTTAGTAAATACTGTAC2260.10285677876235079No Hit
TGCCTGGGGCGATCTTGGCGTGGGTATATAAAGTGGTTATTTTCCTAAAGAC2260.10285677876235079No Hit
TCAAATGGGATTTTTCAAGGGGCAGAAAAATAATTGTTGGTTGATAAGGTAA2250.10240166027225188No Hit
CAAATTGGGGTGTCTCGCAGTCGTGGACTCGTGCAGCTGGGGCGTCCGCAGC2250.10240166027225188No Hit
TTTGCTGGGGATCTAATAATGGGCAGTAATAGCATCTAAATATCACAAACTC2250.10240166027225188No Hit
CAAAATGGGAGGAAGTCTCCACTTCCAAGATCTAGGAAGACGGCTACAAATG2250.10240166027225188No Hit
TGCAGAGGGTACTATGTAACAATTTTTTTTTCTTTGAGACAGGGTCTCACTC2240.10194654178215298No Hit
TAGATGGGGTATATTTGATTTATTAACTTAATAATCAAGAGCCTTAAAACAT2240.10194654178215298No Hit
GCTCACTTCTGGTCTTTTTGGCCGGGAACTCCTGATTGGTGTTAAGGTGGTA2240.10194654178215298No Hit
CTGATTTCGGGATCACCAACATCTGATAAAATCTCATTGAAGGCAATGCCAT2240.10194654178215298No Hit
GACAAAGGGATTCTTTACTGCTAGATTAGAAAGACCTAATGTGAGGTTAGAA2240.10194654178215298No Hit
AGGTCAGGGATAATTTCACACAATTGTACGTTCCTTGCTGCTGGTCTTATCT2230.1014914232920541No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGG2230.1014914232920541No Hit
AGTCAGGGGCAACAATGAAATGTCAAAATGTAATATATTCTTTAAAGCAAAT2230.1014914232920541No Hit
GTGTGCGGGAACTATTCTCGGCTCTCAGAGTATGCCAAAATCGAAGGGAAAA2220.10103630480195519No Hit
ACCTGTGGGGTGATACCTTTATCTTTCTGAAAGTAGATTCTGGGTCGGGCAC2220.10103630480195519No Hit
CAATTTGGGCGGGGACTGGACACAAACAGGAATGTCTGGAGTGGTAACAGCT2210.10058118631185628No Hit
GTACCGGGGACTCACCCAAGTTCCAGATTTGTATTTCACGCACAGTTCCATG2210.10058118631185628No Hit
GCATAGGGGGCTTTGGAGGGAGTGGAGCTTTGGAGCCTGGAGACAGGGGACC2210.10058118631185628No Hit
TGAAGCGGGAGTTCAGTGCCTGTGAGTAGAGCTGACCGGCCGCAGTTCAGCC2210.10058118631185628No Hit
GTATGAGGGAAGAATGGGGATCACAGAGACATTCCTCCGAGCCGGGGAGTTT2200.1001260678217574No Hit
ACTGCCGGGGAAATTCAAGGGCTGAGGGGTGAGTTGGTCTGTGGGTAAAATT2200.1001260678217574No Hit

[WARN]Adapter Content

Adapter graph