FastQCFastQC Report
Sat 31 Dec 2022
SRR5847222.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847222.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219722
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTACTCGGATCTCGTATGCCGTCT16870.7677883871437543No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGGATCTCGTATGC14260.6490019206087693No Hit
AATAGCGGGAATAAATGAAGGCATTAACTTTTCTCTCCCATGTGAGTGAAAT3320.15110002639699255No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC3270.14882442358980894No Hit
TCACACGGGTATTTTCTCCATTAGTAATAGTAATATTTTGATATCTTAATAG3110.14154249460682136No Hit
GCCGCAGGTACACATGGAAGATGAAGAATTACAACAATGAAGAAAAGTCCTC2930.13335032450096032No Hit
CTGCTTGGGATCTCCCAAGAATAAGGGCATTTTCCTACAAAACCACAATGCC2920.1328952039395236No Hit
ACGCTTGGGTCTGGTTGCTTAAGCTACATGTTTGAGAGAAGTTTCTAACCCT2840.12925423944802977No Hit
TCAAGAGGGGATGTGTTCACATAATGAAATTTACTGGTCAAGTCTATCTGTA2830.12879911888659307No Hit
AGAGCAGGGCTCTGTTCCAATCTGCGTGGTGCTTCTTTAGTAAATACTGTAC2760.125613274956536No Hit
AAGCGTGGGAAGAAATGCACAGGCGTGCATGGCATGCACGTTCAGACAGCTG2760.125613274956536No Hit
GTTAAAGGGCTTGGAAATAATTTGCTATCAAACTGAAAAAAAATCTTGAATG2760.125613274956536No Hit
TAGTTCGGGGATGGGACCTTGTCACTTTGAGCTAAAACCAGGCAAAGCTCAC2750.12515815439509925No Hit
ATTAGAGGGTCTAGGGAAGATCAGGTACATTGCAGAATTCAAAGCATGGTAA2750.12515815439509925No Hit
TTAGCGGGGCTTCCGAACCCCCATCCCTACCCCTGGCTGAGCTGGGCTGATC2720.12379279271078909No Hit
TGCCTGGGGCGATCTTGGCGTGGGTATATAAAGTGGTTATTTTCCTAAAGAC2720.12379279271078909No Hit
GCTGTTGGGGGAGATCGGTGACGAAGTCCGCATCCCGCCGGTGCGCGTGCCC2700.12288255158791564No Hit
AGACATGGGGAGGTTTAAGGCTTTGAACTTTTTGAAACTTTGATATAATTGT2700.12288255158791564No Hit
GTCGATGGAGTTGAATAACTAGCACGAGGTCACTCACCTAAAAAGTGGTGAG2680.12197231046504219No Hit
AAAGTCGGGCACGGTGCTGTCGGGCGGCACCACCATGTATCCGGGCATTGCC2680.12197231046504219No Hit
AAGCGTGGGATAACTGACTGCCAAAACAAAGCCCAGCACTTTAAAAGAAAGA2680.12197231046504219No Hit
ATACGGGGGTCTGCAAAGCTCCTCATTCAGTTATTCTGTAAGTTGATCCAAA2660.12106206934216875No Hit
TATTAAGGGTATGTATATTATGTCATTTAACGCTCACAACCACTCCATGTGG2660.12106206934216875No Hit
TGCTATGGGGAAGAAACAAAATCTAGTAATTTGGGGACAGCTAAATCCCACA2610.11878646653498512No Hit
AAGCCTGGGCAACAAGAGTGAAACTCCGTCTCAAAAATGAAAATAAAATAAA2600.11833134597354839No Hit
TTTGCTGGGGATCTAATAATGGGCAGTAATAGCATCTAAATATCACAAACTC2600.11833134597354839No Hit
CGACGGGTGTGTAGGTAATAAAGAATCTGTTCAAATATGCATCAGTGGTATG2590.11787622541211167No Hit
GGTCGAAGGTGACCAGTGTCTCAGTTCTCTTAATTCTATCAACAGCTTTGTT2590.11787622541211167No Hit
TAGAGGGGGAAAGCAGTGTGCATATCTTGAGGGGTGATGAACACAAGCAAAC2590.11787622541211167No Hit
AACACAGGGGAAAGGGAAACACCCTCAATTAGGAAACATTCCAAGGGGAGAA2580.11742110485067495No Hit
TGTGCTGGGGAGCGCGGAGGCAGCCATGGTGCGGTTCAAGCACAGGTACCTG2570.11696598428923821No Hit
TTTACTGGGTACCCCTGAAGAAGCACAGACTTCGGATGTACAAAACAAAGAC2560.11651086372780148No Hit
AATATGGGTTTGTACAGTCTTTTAGACCTATTCAAGTGATGCTCATGATCCT2560.11651086372780148No Hit
TGTCAAGGGGCCGTATGCAGGTGGACCCCCAAAAGTACAAGGGCATATTTAA2550.11605574316636477No Hit
TAAAGCTGGGGAGGGAGAATGGGGGAATATGATGAATTTTGCATTTTATTGG2550.11605574316636477No Hit
TTTACTGGGACCTGAATCATCTAAAAAACACGGTCTCAACCTGGCCACCGTG2550.11605574316636477No Hit
TGCGCAGGGGGTTGCATAAATTTGCGCTCCGCCAGCCCGGAGCATTTAGGGG2540.11560062260492804No Hit
TCGATTGGGATTGAATAAGGTATTCTTGTCAGTTTAGAAATGGACTGGATAA2530.1151455020434913No Hit
TTATACGGGGAGTCCAGATCAAAAATGGCGGTAGTTGGTGTGTCCTCGGTTT2520.1146903814820546No Hit
GTGCTTGGGATGAGATAGAGACAAACACTATGTATTTCTGATGCTTTCAGGT2480.11286989923630769No Hit
TTATCAGGGAATGAAAAACTGCTTCTTAGTTTACTACATGGTATAGGCCCTT2480.11286989923630769No Hit
GAAGTAGGGGTAATTGCTGATACTAGGAATTAAGGGATTTTTTAAAAGTAGC2470.11241477867487099No Hit
TACGTAGGGGATTTTGGTATGCATAGGAGGTCCTGGAACCAATCCGCTATGG2470.11241477867487099No Hit
TTGCGAGGGGTAGTCTCTGTAGCATCTCCCCAAAGTGTTAAACAGCTACATA2470.11241477867487099No Hit
TAAAGCGGGGTTTCTGCATGTCAGCGAGCGACACTGTCCCTGCCTCAGGTTG2450.11150453755199752No Hit
ACTGCCGGGGAAATTCAAGGGCTGAGGGGTGAGTTGGTCTGTGGGTAAAATT2440.11104941699056081No Hit
CTTAATGGGATAGATGTACAAGGAAATGCATTTTCTGCCTCCACCCCCTTTT2440.11104941699056081No Hit
AATATTGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTG2430.11059429642912408No Hit
ACCTGTGGGGTGATACCTTTATCTTTCTGAAAGTAGATTCTGGGTCGGGCAC2430.11059429642912408No Hit
CCAGGCTGGGATTGCAGGCGTGAGCCACCGCACCCAGCCGCCAGTCACTACT2410.10968405530625064No Hit
TCAAATGGGATTTTTCAAGGGGCAGAAAAATAATTGTTGGTTGATAAGGTAA2410.10968405530625064No Hit
GCAGTGGGGCCCAATTCAATAGGTGGAGCACAAAGAAGAATCATTTGATTTC2400.1092289347448139No Hit
GGCAAGGGATTCTACAGGCTACAATCTAATGCTAACAGTTTATTTGGTTGCT2380.10831869362194047No Hit
TAGTTAGGGTACCAATGTTCTTGGGTCACTGAAGAAGCTACTACCATGAGCC2370.10786357306050373No Hit
TTTCCAGGGCTCAGGTCACAGGGATGCAGCTTTGCTCATTGCTTACACAGCC2360.107408452499067No Hit
GCAAGTGGGGAGATGCCCTAAAGATGAAGCTCAACAAAGCACGAAAAGATCA2360.107408452499067No Hit
AAGCAGAGGGGGGGGTGGGATCTGAACATGGCGGCGGTGGTAGCTGCTACGG2350.10695333193763029No Hit
GCGGTCGGGAGAGCTTCCTATTAAGACCACCACATCTACTCATCTTTAAGCT2340.10649821137619356No Hit
GTACCGGGGACTCACCCAAGTTCCAGATTTGTATTTCACGCACAGTTCCATG2340.10649821137619356No Hit
AAGTACGGGTAAAAGTAGAGGTTTTGTAGGGGTACAGATCAATAACTGTCAC2340.10649821137619356No Hit
TTCGGCGGGGCTGTCATATTATGTATTTGGCTTGTAACCTACTTTTTATCAT2340.10649821137619356No Hit
GTTTAAGGGCCACTCTTAATGGGCTTATTCACCATTTGTGTAATTCATTAAT2340.10649821137619356No Hit
TCTCGAGGGGATGCCAATAAATATGACTAAGAACTTTCATGCAATTGACAAA2330.10604309081475682No Hit
GCTTCAGGGATGATTTCTATGCATATTTATGGAGTTTTTTTCCTATGAAACC2330.10604309081475682No Hit
TAATACGGGTGGATATATGTATTTCCCATTGGTCTAGCGGATGTCTTAAAAG2330.10604309081475682No Hit
GCTTGTGGGATATGTGAATGTCAGAGCAAGGATGGAACGGATAAACTGCCTA2330.10604309081475682No Hit
GACACTGGGAAGCAAGAAAAGAAACGCCAAAAACGGAAGAATAGACGAAAAA2320.10558797025332012No Hit
TAGTGAGGGATTTCCTTCTTATACATTAAAAATAGTGTGAAACATGATTGAA2310.10513284969188338No Hit
GTGGCTGGGAGGTGAGCCCCAGTGACAGTCTTAAGCACAGTGATTGGCAGAT2300.10467772913044665No Hit
ACTTAAGGGATTTATTGATGTATAAAAATAGGATAATGGGGAACTGGACTGA2300.10467772913044665No Hit
CACTTAGGGCAGTTTTGATGTCTTACCTATTTTGTTTTTTTCTCCCTTGAAT2300.10467772913044665No Hit
AAGTGGGGGGATGACGCTGGGATAACTTTCTAGCAATAAGTATAGGATTGAA2290.10422260856900994No Hit
ACTGAGGGAATACTAAAGCTTATTGTTAAAGGAAGGCAGAAAAATTAAGTAA2290.10422260856900994No Hit
GCATAGGGGGCTTTGGAGGGAGTGGAGCTTTGGAGCCTGGAGACAGGGGACC2290.10422260856900994No Hit
CTGATTTCGGGATCACCAACATCTGATAAAATCTCATTGAAGGCAATGCCAT2290.10422260856900994No Hit
TTATGTGGGTGGTACCAGTCCATGGCCAGTTAGGAACTGGGCTGCACGGCAG2290.10422260856900994No Hit
CACAGTGGGGTGAGATACAAAAGTAATTGCAGGCCCAGGGCTGATTATTTAT2280.10376748800757321No Hit
TAACGAGGGAAGATAGAGATGTTTTAAAGGCTTCTGGGTTTGATTTCTTAGT2280.10376748800757321No Hit
CTATTTGGGGATCTGTGTTCGGGAGTGCCTGGAGTCAAATATAACATTCCAA2260.10285724688469976No Hit
GACCATGGGGGAACAGAAACAGGCATTCCTCCACAGTGAGTGGCTCCCCCTA2260.10285724688469976No Hit
GCAAATGGGTTTCTAATTTTCCAACTAAATATCCCATTATTTAATCCACTAT2250.10240212632326304No Hit
ATTTCGGGGACTTTGTTTTCTTTTAACCTGCTAAGTGGCATTCGGGAAACTT2240.1019470057618263No Hit
GTGTCTGGGGCGCAATCTCAGCTCACCGCAACCTCCACCTCCTGGGTTCAAG2240.1019470057618263No Hit
TCGACAGGGATATTATCATACTAATTGTACTTTTATATAAGATGCAGTACAT2230.10149188520038958No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGG2230.10149188520038958No Hit
GACAAAGGGATTCTTTACTGCTAGATTAGAAAGACCTAATGTGAGGTTAGAA2230.10149188520038958No Hit
CGCGTTGGGATATGTTGAAATTTTGTGGAATAATACTACCTGTAGAAAGTTG2220.10103676463895286No Hit
AGGTCAGGGATAATTTCACACAATTGTACGTTCCTTGCTGCTGGTCTTATCT2220.10103676463895286No Hit
GCCCTAGGGATACTTTGGAGGATCACAGATTGTTCCATAACCTTAAGCCTCC2210.10058164407751613No Hit
ACCTGTGGGTCTTGAGTGGGGAAATGTTACCAAAGGAATTTCTGAGATACGT2200.10012652351607941No Hit

[WARN]Adapter Content

Adapter graph