FastQCFastQC Report
Sat 31 Dec 2022
SRR5847221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217075
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTACTCGGATCTCGTATGCCGTCT16680.7683980191178165No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGGATCTCGTATGC14290.6582978233329494No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC3060.1409651042266498No Hit
GTTAAAGGGCTTGGAAATAATTTGCTATCAAACTGAAAAAAAATCTTGAATG2910.13405505009789243No Hit
AAGCCTGGGCAACAAGAGTGAAACTCCGTCTCAAAAATGAAAATAAAATAAA2890.13313370954739145No Hit
TCACACGGGTATTTTCTCCATTAGTAATAGTAATATTTTGATATCTTAATAG2890.13313370954739145No Hit
TTTACTGGGTACCCCTGAAGAAGCACAGACTTCGGATGTACAAAACAAAGAC2820.12990901762063803No Hit
CTGCTTGGGATCTCCCAAGAATAAGGGCATTTTCCTACAAAACCACAATGCC2800.12898767707013706No Hit
ATACGGGGGTCTGCAAAGCTCCTCATTCAGTTATTCTGTAAGTTGATCCAAA2770.12760566624438557No Hit
AATAGCGGGAATAAATGAAGGCATTAACTTTTCTCTCCCATGTGAGTGAAAT2770.12760566624438557No Hit
AAGCGTGGGATAACTGACTGCCAAAACAAAGCCCAGCACTTTAAAAGAAAGA2730.12576298514338363No Hit
AGCTGGGATTGAAATCCTTTTCAGAAAAAATATAATTGCAAATAGGTAGGAG2720.12530231486813315No Hit
CGACGGGTGTGTAGGTAATAAAGAATCTGTTCAAATATGCATCAGTGGTATG2710.12484164459288265No Hit
GTCGATGGAGTTGAATAACTAGCACGAGGTCACTCACCTAAAAAGTGGTGAG2700.12438097431763215No Hit
GGTCGAAGGTGACCAGTGTCTCAGTTCTCTTAATTCTATCAACAGCTTTGTT2680.12345963376713118No Hit
TTATACGGGGAGTCCAGATCAAAAATGGCGGTAGTTGGTGTGTCCTCGGTTT2650.12207762294137971No Hit
ACTTAAGGGATTTATTGATGTATAAAAATAGGATAATGGGGAACTGGACTGA2640.12161695266612924No Hit
GCCGCAGGTACACATGGAAGATGAAGAATTACAACAATGAAGAAAAGTCCTC2620.12069561211562824No Hit
TGCCTGGGGCGATCTTGGCGTGGGTATATAAAGTGGTTATTTTCCTAAAGAC2600.11977427156512725No Hit
TAGTTAGGGTACCAATGTTCTTGGGTCACTGAAGAAGCTACTACCATGAGCC2570.1183922607393758No Hit
ACGCTTGGGTCTGGTTGCTTAAGCTACATGTTTGAGAGAAGTTTCTAACCCT2560.1179315904641253No Hit
CTATGTGGGCAAGTTAATAACCTAACATCACAACTAAAAGAACTAGAGAACC2550.1174709201888748No Hit
TGCTATGGGGAAGAAACAAAATCTAGTAATTTGGGGACAGCTAAATCCCACA2530.11654957963837384No Hit
AACACAGGGGAAAGGGAAACACCCTCAATTAGGAAACATTCCAAGGGGAGAA2520.11608890936312334No Hit
TAGGCTGGGGTGGATCATAAGCTCTCTGCATTCCCATAGCACCAGGCTTACT2510.11562823908787284No Hit
GCTGCGGTGACAAAGCACTGGGTTTATTATGTATTAGTGCTGTAGTTATACT2510.11562823908787284No Hit
TCAAGAGGGGATGTGTTCACATAATGAAATTTACTGGTCAAGTCTATCTGTA2480.11424622826212139No Hit
AAGTACGGGTAAAAGTAGAGGTTTTGTAGGGGTACAGATCAATAACTGTCAC2470.1137855579868709No Hit
GGCAAGGGATTCTACAGGCTACAATCTAATGCTAACAGTTTATTTGGTTGCT2440.11240354716111943No Hit
TAATACGGGTGGATATATGTATTTCCCATTGGTCTAGCGGATGTCTTAAAAG2430.11194287688586894No Hit
TTTACTGGGACCTGAATCATCTAAAAAACACGGTCTCAACCTGGCCACCGTG2420.11148220661061845No Hit
TGTCAAGGGGCCGTATGCAGGTGGACCCCCAAAAGTACAAGGGCATATTTAA2410.11102153633536795No Hit
TTAAAAGGGGAAATTCATTGAGGAAGGAATCTTATATGAGTTTAGACTTCAT2410.11102153633536795No Hit
ACTGCCGGGGAAATTCAAGGGCTGAGGGGTGAGTTGGTCTGTGGGTAAAATT2410.11102153633536795No Hit
GTGCTTGGGATGAGATAGAGACAAACACTATGTATTTCTGATGCTTTCAGGT2410.11102153633536795No Hit
GCTTGTGGGATATGTGAATGTCAGAGCAAGGATGGAACGGATAAACTGCCTA2400.11056086606011747No Hit
TATTAAGGGTATGTATATTATGTCATTTAACGCTCACAACCACTCCATGTGG2390.11010019578486699No Hit
GACCATGGGGGAACAGAAACAGGCATTCCTCCACAGTGAGTGGCTCCCCCTA2380.10963952550961649No Hit
AATATGGGTTTGTACAGTCTTTTAGACCTATTCAAGTGATGCTCATGATCCT2360.10871818495911552No Hit
CGCGTTGGGATATGTTGAAATTTTGTGGAATAATACTACCTGTAGAAAGTTG2350.10825751468386503No Hit
CTTAATGGGATAGATGTACAAGGAAATGCATTTTCTGCCTCCACCCCCTTTT2340.10779684440861453No Hit
CAAATTGGGGTGTCTCGCAGTCGTGGACTCGTGCAGCTGGGGCGTCCGCAGC2320.10687550385811355No Hit
AACACAGGGAAGAGGAAAGTGAACCACAGACGAGAGAAGAGTTATGGCTGTA2320.10687550385811355No Hit
CGCTATGGGAACCAAGGGAGCATAACAATGACATGAGGGCTTGGAAAGGAAA2310.10641483358286308No Hit
TAGAGGGGGAAAGCAGTGTGCATATCTTGAGGGGTGATGAACACAAGCAAAC2310.10641483358286308No Hit
CCAGGCTGGGATTGCAGGCGTGAGCCACCGCACCCAGCCGCCAGTCACTACT2300.10595416330761258No Hit
CTGATTTCGGGATCACCAACATCTGATAAAATCTCATTGAAGGCAATGCCAT2300.10595416330761258No Hit
AATGCAGGGGTCCCCTCCTGGTTACCTTTCCTTTTCCATTCTATCTCTTTTG2300.10595416330761258No Hit
TTATGTGGGTGGTACCAGTCCATGGCCAGTTAGGAACTGGGCTGCACGGCAG2300.10595416330761258No Hit
GCAAGTGGGGAGATGCCCTAAAGATGAAGCTCAACAAAGCACGAAAAGATCA2300.10595416330761258No Hit
TGTGCTGGGGAGCGCGGAGGCAGCCATGGTGCGGTTCAAGCACAGGTACCTG2290.10549349303236209No Hit
GCTTATGGGCGATCTGTACTGGGGAAGCCATACAAAAAATAAGCAAAAGTGT2290.10549349303236209No Hit
AAAGTCGGGCACGGTGCTGTCGGGCGGCACCACCATGTATCCGGGCATTGCC2290.10549349303236209No Hit
CAGCCGGGTGTATTTGCTAGGGTTGCCATAACAAAGTACCACACATTGAGTG2290.10549349303236209No Hit
GAAGTAGGGGTAATTGCTGATACTAGGAATTAAGGGATTTTTTAAAAGTAGC2270.1045721524818611No Hit
AAGCGTGGGAAGAAATGCACAGGCGTGCATGGCATGCACGTTCAGACAGCTG2270.1045721524818611No Hit
TGCGCAGGGGGTTGCATAAATTTGCGCTCCGCCAGCCCGGAGCATTTAGGGG2270.1045721524818611No Hit
TAGTTCGGGGATGGGACCTTGTCACTTTGAGCTAAAACCAGGCAAAGCTCAC2260.10411148220661062No Hit
TTTGCTGGGGATCTAATAATGGGCAGTAATAGCATCTAAATATCACAAACTC2260.10411148220661062No Hit
ACACACACACACACAAAACAAGATTGTAACAAACTTCTTTCATTTTGTTGAT2260.10411148220661062No Hit
AAGCAGAGGGGGGGGTGGGATCTGAACATGGCGGCGGTGGTAGCTGCTACGG2250.10365081193136014No Hit
GCAGTGGGGCCCAATTCAATAGGTGGAGCACAAAGAAGAATCATTTGATTTC2250.10365081193136014No Hit
ATTAGAGGGTCTAGGGAAGATCAGGTACATTGCAGAATTCAAAGCATGGTAA2250.10365081193136014No Hit
TCTCGAGGGGATGCCAATAAATATGACTAAGAACTTTCATGCAATTGACAAA2220.10226880110560867No Hit
TTAGCGGGGCTTCCGAACCCCCATCCCTACCCCTGGCTGAGCTGGGCTGATC2220.10226880110560867No Hit
ACTGAGGGAATACTAAAGCTTATTGTTAAAGGAAGGCAGAAAAATTAAGTAA2220.10226880110560867No Hit
CACAGTGGGGTGAGATACAAAAGTAATTGCAGGCCCAGGGCTGATTATTTAT2220.10226880110560867No Hit
TACGTAGGGGATTTTGGTATGCATAGGAGGTCCTGGAACCAATCCGCTATGG2210.10180813083035818No Hit
TTGATTGGGACATGTGGCAGAAAAGAATAAACCCACAGAGACCATAGGGCAT2210.10180813083035818No Hit
TCGATTGGGATTGAATAAGGTATTCTTGTCAGTTTAGAAATGGACTGGATAA2200.10134746055510768No Hit
CGCACTGGGGGCGAGACGGCTGGGCGCCGAGTGGGACAGCGCTGGTGCGGAG2200.10134746055510768No Hit
CGATAGGGCACTCCTGCCTGCTCACACCCACTTTGCTCTAGTCATCCACACT2200.10134746055510768No Hit
TGAATGGGACCTGTGAATAGTAGCATCATCGCTGCTCCCATCACGGACCCTT2190.10088679027985718No Hit
TAAAGCTGGGGAGGGAGAATGGGGGAATATGATGAATTTTGCATTTTATTGG2180.1004261200046067No Hit
TATCAAGGGAATTAACTGTCAAGCCTGTGGATGATTTTTTTGAACTTGGTAG2180.1004261200046067No Hit
AGACATGGGGAGGTTTAAGGCTTTGAACTTTTTGAAACTTTGATATAATTGT2180.1004261200046067No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)