FastQCFastQC Report
Sat 31 Dec 2022
SRR5847197.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847197.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184979
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTACTCGGATCTCGTATGCCGTCT25351.370425832121484No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGGATCTCGTATGC22451.213651279334411No Hit
ATGGCCGGGCTGAGGATTGTATTTCAGAACCACCAACTGCTCTTGACAATTG3140.16974899853496883No Hit
TACACTCGGGATGCAGTATTAGATGGCAATAAGCCTCCTTGCAATAGCAGCT3110.16812719281648186No Hit
ACACTAGGGGATTGTTGATGATGCCACAGCAGGTTACTAAATTCTGCTCAGA3070.16596478519183258No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGG2990.16163996994253402No Hit
ACGATTGGGGCTGAAAAAATTCTTTGGAAACACCTCCCTCATCAACTCCTTG2770.14974672800696295No Hit
GTCCTAGGCAGACACGTAAGTCAATGGAATGGAGACGAGAGATTTCTACAAA2680.14488131085150208No Hit
TTGCTTGGGATTCTAATCTGATTGATTCAAGTTTAAGTTGCTCATAATTTTT2660.14380010703917742No Hit
TGAGGTGGGATGAACATTTGGTACATGTGCCAGTTCAACTTATTCTTCATAA2470.13352867082209333No Hit
TAAGTTGGGGTGCGCACATGCAGCTTTGCTTTCCTTAATACATTATCTGCTA2460.132988068915931No Hit
AGCGTAGGGGAACTGCTTTGAGGTGCCTTGGAACTGGCTTTGGAAATAGCTA2460.132988068915931No Hit
AACTAGGGGGACGCCCTGTGGATGGCCACTCTGCCCATCAAGCTGCCGGTGG2440.13190686510360636No Hit
TCGTACGGGCCAAACCCAAAATTGGTAACAGAAAAGAAATAATAGAGATCAG2400.12974445747895708No Hit
TACATGGGGGCTGTTTTAATGGACAGATTTGAATGTGGCTTTCTTCTTATAG2400.12974445747895708No Hit
TACGTAGGGACTCTTGACACAGATAACTTCAGACTGATCTTCTGTCCAAGGA2380.12866325366663242No Hit
ACACTAGGGATTCCATGAATGCTCCTGCTTCAGGGCCTCTGCATTTGCTGTT2350.12704144794814545No Hit
TCGGACGGGAGCATTTGTAAGATGCTGCACAGGAGCAGCATTATCCCCAAAG2340.12650084604198314No Hit
AGAAATGGGTTATGGCTCTATTTTGTAAAACAAACTACTGGTAATTATTTTT2340.12650084604198314No Hit
TCAGCATGGGGATTCAGATCAACTGGTGTTGGAAGTTATGTAGAGATGTCAT2320.1254196422296585No Hit
ACAGTGGGCCACGTGGGCAGCCTTCTGCTTATGACAATAATTGGCCCATTGC2310.12487904032349617No Hit
GAACCAGGGGGTGGTACATGCCTGTAGTCCCAGATGTTCAGCAGACTGAGGT2290.12379783651117154No Hit
AGATTGGGGGGAGGCGGCGGCGGGCGCGCGGCGGCGGCGGGCGTGCGAGGCT2290.12379783651117154No Hit
CAATACGGGTCAGGCGTGGAGGAACCATGGTAACCTATGGGGGGATGGCCAA2210.11947302126187297No Hit
TTGCATGGGAACTATAACATATTCATACAATGAGCTCTTACTGCTTATTTGC2210.11947302126187297No Hit
CGGTGAGGGACACATGTATTTTCTCCATAAGGTATATCACAGCCATCTTGCA2210.11947302126187297No Hit
TTGTTAGGGGTGGGGCTGCTTTTATCTCAAAGGTAACCCATCTACCCTGCTC2200.11893241935571064No Hit
GCTTACGGGCAAATCAAGGATAAATATCTTACGCACGATATCTAGTGACAGA2200.11893241935571064No Hit
GACGCAGGGGAGGTGACTATGTTTTTTAACTTTTATTTTAGGTTCAGGGGTA2180.11785121554338601No Hit
CGTTATGGGGTGTCCCAACAACGATATGAAAGAGCTTGTTGGATGAAGTCTT2180.11785121554338601No Hit
CCTTTAGGGCTGCAGGGCGGGCAAGCAAGCTTACAGAAGCAGAACAAAGGCA2180.11785121554338601No Hit
AAGACAGGGCTTGTTTGTTTAATAATAGAACTGATGAGTAGTTAATTTGAGA2180.11785121554338601No Hit
CGACTAGGGCGGGATGGAGGAGAGAAGAGGGTAACATTTCAAAGGTGCCCTC2140.11568880791873672No Hit
ACCCGAGGGCACCCAGACACTCTGAGGGCTTTATGATCCATTGTGAGGGTTA2130.11514820601257439No Hit
CTAAATGGGAAGGAGAACTACAAACCACTGCTCAACGAAATAAAAGAGGACA2120.11460760410641209No Hit
CACCTAGGGTCCATTATCAAAATTGACTGGGGATTAACACCGATGTTAGAGT2120.11460760410641209No Hit
ATGCAAGGGTGTTAAAGAGAAGCCAAGTCTAGAGAAAGCAATCCTGTAACCC2110.11406700220024976No Hit
TTCAACGGGCTAATAGAAAAGTCATGGTTACATCAACTGGATACATACAGCC2110.11406700220024976No Hit
GTTCCGGGTGAAGACACTTTGTCATGAAGGTTGTAAAGCACCAAATATTTTT2110.11406700220024976No Hit
TCCGCTGGGGTGATACTGTTAGCATTCAAAACAAAGAACAAATTAGTAATGG2100.11352640029408743No Hit
CTACGAGGGCAAACTCCTGACCTCAGGTGATCCACCTGCTTTGGCCTCCCAG2100.11352640029408743No Hit
AATTAAGGGTCTTGAGTTTCTTGTTAATGATGGAAGATCCACCTTTATTGGT2080.1124451964817628No Hit
GATTCAGGATTAATAATATTGCTGGGGAATGGGGATGGCCTATGCAGGAATG2080.1124451964817628No Hit
TGTCTAGGGGATTGCAAACACAATATATAGCTCAAGTTTACTTTGTACTCAA2070.11190459457560047No Hit
TACATCCGGGATGTGTAATAACCTTTAATAAAAATATATCCTCAGTTTTTCC2050.11082339076327584No Hit
TATTCCGGGGTTTAATCTTATTATATTGATAGTTTCATAAACCTCCTTTTAC2050.11082339076327584No Hit
AGCCTTGGGTGATCAAATGACAGTGAACAAAGCCAGCCCAAGCTGGTAAGGT2040.11028278885711351No Hit
GTGACTGGGTCCTCTCACCTCATTTTGTAAATGAAAAATCCCTAATTCAGTG2040.11028278885711351No Hit
GACTTTGGGGGGTTGGCATGAGGAGGGGGAAGCTTTTTTGAATCGCTTTTTG2030.10974218695095118No Hit
TAGTGCGGGGGCTGGAGTGGAGTGCAAATGGCTTGATCTCGGCTCACTGCAA2030.10974218695095118No Hit
TAGTTAGGGTACAACCCTTCGCTGACGCCATAAAACTCTTCACCAAAGAGCC2030.10974218695095118No Hit
TAACACGGGCTCAAGTCGGGTTTCTTTCATATTTTCTGACTTCCTTGTTATT2030.10974218695095118No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGGAACTCGTATGC2020.10920158504478887No Hit
CGTGGCGGGAGGCCTCTCTCCTGATACAGCAGGCGCTCACCATGTTCCCTGG2020.10920158504478887No Hit
CTTCAAGGATTACTGGATAGAATTCAGGCTTTCCCCTCCAGCCCCCAATCAT2020.10920158504478887No Hit
TCTTCAGGGCGCTCTTCCGATCTTATGCTGGGATGACAAGGTGAATTTCCAT2020.10920158504478887No Hit
GATGTTGGGATATGTTAGTTAGGACAAACGTATTTTGCTTTCCCATTAGCCT2010.10866098313862654No Hit
ATAATAGGGCTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCG2000.10812038123246422No Hit
GTCGCAGGGGAGGGAAGATTTCACTTCATTGTCTAGCCCAGAATCTTGAGCA2000.10812038123246422No Hit
GAGTACGGGTCTCTAACCACAGCGGGAGAAAATTTTCTGGTTATCAGATTGG1990.10757977932630189No Hit
GTAATAGGGGAAAGTAATTGACACTTTGGCATTGAATGAGGTTACAACAAAT1990.10757977932630189No Hit
AGTTTTGGGGGGAACATCTGAATGCTGAGGCCTAGAGATCGTTCAGGGTGTT1980.10703917742013959No Hit
CTATTTGGGACTTGGGACCAAAAAGAATGCATAACTTTCCAATTTCCTTTAA1980.10703917742013959No Hit
GCCCGAGAGAATGTGTGGAAGTTCTTCACCATTTTCACTCCTATATTTTTCT1980.10703917742013959No Hit
AGTTACGGGAGAGCTGTCTGAGGGCTTCCAAAAGAGCTGTTCAGTGAATAAG1980.10703917742013959No Hit
GCAATAGGGATGAAAGGAAGCTAAGAACCTTGATAAAAGGTTAGAGGAATTG1970.10649857551397726No Hit
CTCAACAGAGAAAGGTGAGAGTCTGTTATTTGTTAGGTTTCCTTGTCCATAT1960.10595797360781493No Hit
CGGAGTGGGGACTCGGTGCTACTCACACACATTGGGGCAGACAACCTGCCAG1960.10595797360781493No Hit
CATGGCGGAGTTCATATTGACAAAGGCATGAAAGATGGCCAGAAGATAACAT1960.10595797360781493No Hit
TGTTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1960.10595797360781493No Hit
CTCTGGGGGACATCAAGAAAGGATGAACCCATGGAGGAGCTTAAAGATTGGT1960.10595797360781493No Hit
TACAGTGGGTCCTGCTTTGTAGTCTCTAACTCTGAAATTTAAAATATGTAAA1960.10595797360781493No Hit
AGTTCAGGGATTACTGCTCAAAATAAAAATTGTCTACTGAGGAAGCTAGATG1950.10541737170165263No Hit
TACGATAGGGGGCCAGGTTAGTGGCTTCTCAAGTGGAGACAAGTTTGACCCC1950.10541737170165263No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTACTCGGA1950.10541737170165263No Hit
ACGTACGGGATCTTTTAACCAACTGAACAATACACCAAAAGCAGCCTAGGGA1950.10541737170165263No Hit
CGCAGGGGGTTCTGAACTCCCAGATCTGAGAAGGAACTAGTGTTGGTGGTAT1950.10541737170165263No Hit
TTTATAGGGATGGGGTTGGCATTGAATCTATAAATTACCTTGGGCAGTATGG1940.1048767697954903No Hit
AATCCTGGGGCTTTTATTAAAATACAAAATTAAACAAACAAAAAAACTCATG1940.1048767697954903No Hit
TACTGTGGGGTTATATATGAAAGATTCGTAGTAATTGATAGGAAAATCTTAT1940.1048767697954903No Hit
TTGCTTGGGATCGTCAAATCAAAGCCACCCTCAGTTTTTTGTATGGTGCCAT1940.1048767697954903No Hit
AGACCGGGAGATAACAAGAATAAAAATTGAATTGGAGGAAGCCCTAGTCAAT1930.10433616788932798No Hit
TTATCTGGGAACTTGAAACTCTTATCAAAATATATTTTACCAGTTTCCAGAA1920.10379556598316567No Hit
ACACACGGGGTTATAATCTTGAACCTTAAACTTTCCAAGGATTGAGATTTCA1920.10379556598316567No Hit
AAACAGGGAGTGGTTGATTTGGGGGAATCTGGGGGTTTTGTTTTTGTTATTG1920.10379556598316567No Hit
AGCTTCGGGGTTGCTCGGGCAGCCCAGCAGCGGCGCGGCCGCCGAGCCCCTG1920.10379556598316567No Hit
TGCTGTGGGACCTCCTTTATGAAGACCTTCATAAATTCTAGGATGGTGAATT1910.10325496407700334No Hit
ACGGAGGGGTGTTAGGTACAATGACAGTCTCCATTTTCTAGGTAAGAAAACT1910.10325496407700334No Hit
TGTTTAGGGCTGGCATAGGGCAAGCAATTCTTCTGCCTCAGCCTCCCAAGTA1910.10325496407700334No Hit
CGCAGTGGGCTGCTTACATAAGATGAAATAGATTTTAAGTCCAAACCTGTCA1910.10325496407700334No Hit
GTTGTAGGGCTGTTCCACTGATCCATTTGTCTGTTCTTTTGCCGATATTATA1900.10271436217084101No Hit
CCATAGGGGAGGAAAGGACGTTGAATTTGAAGTTGTTGGTGATGCCCCGGAA1900.10271436217084101No Hit
ACCGCCGCTCCAGCGCCGCGCAGCCACCGCCGCCGCCGCCGCCTCTCCTTAG1890.10217376026467868No Hit
CCTGACCGGGCAATTGGAAACCATGTGAGAACAACGCTTCAAATGTATTAGA1890.10217376026467868No Hit
AACGCAGGGATGGGGATGGTCATTCACCACGGTCCAGAGGGGCAGAGCAGGC1880.10163315835851638No Hit
AACGCTGGGTACAGGAATCACAGCTCTCAATAGCATAGAGGGTGTTTATAGG1880.10163315835851638No Hit
AGTCACGGGGCCTGTGACCGGCTTGGCGGTTGGAGCGGCTGTCGCCATGTTG1880.10163315835851638No Hit
TGTCGCTGGGTATGTAACTTTACTTATGTGGGACCAATGGCCAGTATATTCT1870.10109255645235406No Hit
GTTTATGGGATCCTGAGTTATTTTGGTTTCCCGTTATGTGAATATCCTTTTC1870.10109255645235406No Hit
GCTCTAGGGAATCAATTCAAATTGGATCCCAAATACTGAAATGAATTTTTGG1860.10055195454619173No Hit
GCAATGGGGCTTACTCAGAGATGGCAACTGCAAGTAAACTACAGATTAGGAA1860.10055195454619173No Hit
AGGTAGGGGAGTGGTATGAAAGGAAGATTGAAGAAGCACGAAGCCAAGCCAA1850.10001135264002942No Hit
TGTCTAGGGGGGGATTAGAAAAATGAGGCACTGGCTACCAGTAACCTATATA1850.10001135264002942No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)