FastQCFastQC Report
Sat 31 Dec 2022
SRR5847187.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847187.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105625
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC5860.554792899408284No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT5400.5112426035502959RNA PCR Primer, Index 34 (100% over 21bp)
AAAACTGGGATTAAGAAAAGACTAGAAAAGCAAACACAAAGCACACTGTGAT1980.18745562130177515No Hit
CTGCAATGGGGTTCAAGTACGTCTCAGGGTCTTCAGCCGAGAGGAGGGCAGC1680.1590532544378698No Hit
GAGGCGGGCGGATCACTTGATGTCAGGACTAGCCTGGCCAACATGGAGAAAC1620.15337278106508875No Hit
AGTCTTGGGGTTTAAGAGAGAAAGGCGGAAGAAATGAAATAGAAAAGCAAAA1580.14958579881656806No Hit
GCTGTTGGGGCACCAGAAGATGTCTGCATTACTCATTGCTAAAAATGTGTAG1560.14769230769230768No Hit
TTTTGAGGGCTCCTAACTTTGTCTCCTATTATTCTCTCCTGGGCTTTGTATA1550.1467455621301775No Hit
AGCACCGGGATTGGTTTTGGTAATCGATGTGGAAAACCCAAAGGACCAAGAG1550.1467455621301775No Hit
TCCTCAGGGATGTAGTAGATGGAACTTCCCCCAGAGCCCCAGGCCAATGGGG1540.14579881656804733No Hit
GGTTATGGGAATGAACCTAACCTCATTCTTTGTATCTTCAGTGTTGAATTGT1530.14485207100591715No Hit
AACGTAGGGTGAAATAATTAATGTTGGCAAATATGAGAACAACAAAGTCAGC1510.1429585798816568No Hit
CACGAAGGCTGAGGATCAGAACGCAGCAACCCATCTCATTCGCCACGCTGTG1480.14011834319526628No Hit
GTACGGGGCAGAAGCACGGGCACAGGAGCATGTTAAAGGCCCTGAAAAGACC1430.13538461538461538No Hit
GAAGCTGGGATCCCCATCAAGCTACCAATGACTTTCTTCACAGAATTGGAAA1410.13349112426035503No Hit
TACGCAGGGGCAGATTAGGATGGCCTAATGGTAAAACATCAGGCACATTCTA1410.13349112426035503No Hit
TGTGGGGCATTGAGGTTCATCAATGAATTTGTACATTTCTAGTTCCCTTTGG1380.13065088757396448No Hit
TTACATGGGTATCTTTGAGTTAATTAATCTCACTAGTGTGGATAATATAATG1370.1297041420118343No Hit
ATTGTGTGGCGCAGCCGCAGCAAGAGTGAGAGGAGCAGAAAGCGAGGCAGCA1370.1297041420118343No Hit
AGCCCTGAGCCAAATAGTAGAAAAGCTTGTGTTCTTTGATTCAACCCATTTT1360.12875739644970413No Hit
TCTCAGGGGTAAGAAATATAGTTTACTTTGTAACCTCGTGCATTTATATACA1360.12875739644970413No Hit
GCAATAGGGCATGGTTGGCTACCAGCCCCAAGGGGACAAGGCCAACTTCTTC1360.12875739644970413No Hit
TTATGCAGGGGGAATTAAAAAATATGGTCCCTTTGTTGCAGACTTTGCTGAT1360.12875739644970413No Hit
TCGTTCGGGATTTGAGAAATGTGTGGGGACAATGGAGAAAAGTTTTCAGAAA1350.12781065088757398No Hit
GAACAAGGGCGATCTGCAAGTGGGGACCTCAGGTGATCCGCCCGCCTTGGCC1350.12781065088757398No Hit
GCTGCTGGGACTCTCTACAGGACACATTTTACATTCAAATATACAAGTAGGT1340.1268639053254438No Hit
AACATCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1340.1268639053254438No Hit
TGCATTGGGATTCTCTTCATGCTTAAAAAATTTAAAATATTTTTGAAGTCTT1330.1259171597633136No Hit
TAACAGGGAGATTTTTGTATTGATTTGCAAAATGCAGATTATATTTGATAGG1320.12497041420118343No Hit
GGCTTTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1320.12497041420118343No Hit
TCAACTGGGCTACTCCTCCTTATTCCTTTATGTATATCTGTATTTCCATTTG1310.12402366863905326No Hit
GTTGGGGGTGGTATAAAAAAATGAGTATAACCATAGGTTTTGAACAAATTTC1300.12307692307692308No Hit
GCATAGGGGACATCACAATAATATATAATTCAGTCTCTAGTTTCCCTAGAGT1280.1211834319526627No Hit
TATAAAGGGACCTAGGGGAGAAAGAGCCATGTAAATATCTGTAATAAACTTG1280.1211834319526627No Hit
AGTCGGGGGATGGAATAGCTATACTTGTAGTTAAAACTGTTTTCTTATACGG1280.1211834319526627No Hit
AGTCCTGGGGCAGGCAAGAGTGCTGGAGGGCGGCAGCGGCGACCGGAGCGGT1270.12023668639053253No Hit
GAAACTGGGATGTTATAAACTTTTTAATTTAAAAAAGTTTTTAACTTCTTTG1270.12023668639053253No Hit
GGTACGGGTGGTAGTCCATAGGTAGAATGTGTTTTCAAACTCTGAGGATGGG1270.12023668639053253No Hit
GCGACTGGGGGAAGACGACAGCAGTGAGGCGGACTCCCCGAGCTCCTCTGAG1260.11928994082840236No Hit
TTGCTAGGGGCTGCAACTTCCGGGAGGTGCTTGTGTGCCTGGTGCGGGAGCT1260.11928994082840236No Hit
TTCAGCGGACTTCTTAAGCAGCATCCTCATCCTTCTTCCTCTTCAGCTCTTA1240.11739644970414202No Hit
CTTCTGGGGAGAGTTACCAAAGGCTTGTTTTTTCTTGAGCAGTTGAAAGGAG1240.11739644970414202No Hit
TGCTATGGGTGAGCAGGGAGCTGGGAGAAATAAGGAGGACATGAGTACATGG1240.11739644970414202No Hit
TTCGACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1230.11644970414201185No Hit
GCGTATGGGGATTTTGCTGGTCTTTGCATAACTGGCAGAGTGCAAGTTTGAT1230.11644970414201185No Hit
TGTGCCTGGGGACAATAAATTCAACACTTTAGATGAAATGAGCAAAAATTAT1230.11644970414201185No Hit
ATGCTGGGAGTCATGTGACCTATCAACATAGTTGCCTTAACCCTTCACTTCA1230.11644970414201185No Hit
ATGCGCGGGCTCCTTCTGTCGCCCAGGCTGAAGTGCAGTGCTGTGATCTCGG1220.11550295857988165No Hit
CTGCTCGGGCGATCTTGGGATGGGGCTAAAAGGAGATGCCAATGTTGAATGA1220.11550295857988165No Hit
GTATTCGGGGTGGCGAGGGGCACCAGTATTAGAGGCACCGCCTGCCCAGTGA1220.11550295857988165No Hit
CAGTTAGGGATTCAAATTACTTGGAGTATTCTTTCTGAGAAGCATTCTGATA1220.11550295857988165No Hit
CAACAGGGGTTTTAACTACAGGATTTTATACTTTTAATATTATTTGTTATTC1220.11550295857988165No Hit
TTAGCGGGGATACGATGATAGTCAGCCTAAATGAAATCCCTGAAGCATTTTA1220.11550295857988165No Hit
TTTTATGGGAGGAACAAGCTGGCTTCTGTGATTAGGTGAAGGGATGGGGGAA1220.11550295857988165No Hit
GAGCTTGGGATCTCTCATTTTCCCATTGTTTAATTTCTAAGTGGATGTTCAG1200.1136094674556213No Hit
ATCGCAGGGGGTGTCAGTGAGACCTTGCAGAAGCTGAACATACATGTCCATA1190.11266272189349112No Hit
GTAGACGGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGG1190.11266272189349112No Hit
CCACCACGGGGGGTGCTTCTCAGTGGGAAGGTGTAAGGAGGGGCTTGCTAAG1180.11171597633136095No Hit
TAATAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1180.11171597633136095No Hit
CTAGCAGGGCAGAGGCTTCTGCTCGCATACCTGAGCAGCTTGATGTGACTGC1180.11171597633136095No Hit
GACCAATGGGATTCTGGTTCATTAAATTGTTACCAAAACATTTTTGAAAGTT1180.11171597633136095No Hit
GAAGACGGGTGGTTTTTAAAGACTCACCTTTTTAAATGGTACTTGGAGCTCC1170.11076923076923079No Hit
TAAGCTGGGCTGTAACGGGGGTTAGATGCGCCACGGTTTCGGTAGCGACGGT1170.11076923076923079No Hit
ACTCGCAGGGGGGGCTGGTGGAGGCGGGGTCGAGATGGCGGCGCCTTTGAGG1170.11076923076923079No Hit
CTCACACAGGGACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTA1170.11076923076923079No Hit
AATACTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1160.10982248520710058No Hit
TAGTCTTGGGAGCGCCACATCTCCTGAGATTATTTGTACGAATTGGAGCAAT1160.10982248520710058No Hit
CTCGAAGGGGTTGGACAACTTAAAGTGTCAGAGTCCCTTGATTTAGGCACGA1160.10982248520710058No Hit
CAACGGGGAGGAAAATACACGTATATTAAGTCAAATGTACATCTAAAAATTT1160.10982248520710058No Hit
CTCCGGAGGGATCTGTCGACGGGCATAATTTCCTTTTCAGATAGCTCATTAA1160.10982248520710058No Hit
CGTTTGGGGAGATCAAAGCCAGGGAAAGCAATCAGAAGCCAGATTGCATTCT1160.10982248520710058No Hit
TATTCCGGGAAATGAGTACAACTCTAGCATTTTGAAGTTCCAGTTGTAAATG1150.10887573964497041No Hit
GACTCTGGGGTCACAGATGTGAAGTCGATTTCACTTTATAACTAACCAGTCC1150.10887573964497041No Hit
TGCGCAGGGTGCTGTGTTACGTCATTCATTTTCTTCTGGTTCCTTAAGTTTT1150.10887573964497041No Hit
TAACATGGGGAAGAGACAGTGCTCTGCCATGATGACAGGGCACTGCTAGGGC1150.10887573964497041No Hit
TGTCAGGGGGGCTAAAACAAAATTTAATACTGTAAGCAAGTATAAATTTTTT1150.10887573964497041No Hit
CTTACAGGGCTCTGTGAAAGCAACATGAAATGAACTGTAGTAGGTGTGTAGT1150.10887573964497041No Hit
TCTCCAGGGGTTCCCTGGGTTCTCTTTTATTGGATTCCTACTACAGGACAAT1150.10887573964497041No Hit
GTTGCCGGGGCTCTCATTTGCCACAGGTTGGCCAGCTTAAGTATGAAGGGTG1140.10792899408284023No Hit
ACGAACGGGATGAAATTATGCATTACCACATTCAAACACAAATCCATTGTAA1140.10792899408284023No Hit
CATAGAGGGAGCAGATGCTGAGGCAGTTCATTAAACCAGGCCTCAGCTTCAG1140.10792899408284023No Hit
GACACTGCTGGCAGGGCTGCAACAGCATGGATGTGAAAGCATCAGGTAAATG1140.10792899408284023No Hit
TACCGAGGGACGCTCTCCGATCTGGCTACGGGAAGCACATGGCCTCCCATCT1140.10792899408284023No Hit
CGTGGGGATGTGGGTCCACTCATATGCTGTTTTTTAGGAAATATGCTAGAAA1140.10792899408284023No Hit
TAATCAGGGAGGAGCTCTGTTATGCTAAAGGACTGCAGTTTTTTTATTACTG1140.10792899408284023No Hit
TATCGCAGGGATCCTTTTGATACTTTGCATGAAAGACATGGAGGATGATGTA1140.10792899408284023No Hit
CGTATTGGGAAGAAATTTAAACTCTACGTTTGGTTTTCACATACAGCAGCTC1140.10792899408284023No Hit
TATGGTGGGATAAGTAATATAGTTAACAAGCAAAAAAAGTGCTGCCACCTTT1140.10792899408284023No Hit
TTACCTGGGCAAAGGACATGATCTTGTTCCTTTTGATAGGTGCAGCAAACTA1130.10698224852071006No Hit
TAGAACGGGACTCATTACTGCAAATTGATTGTCTTTCCCCCCTCCCTCCAGT1130.10698224852071006No Hit
ATGTCAGGGGCCCACCCACATAATGGAGAGTAATCTGCTTTACTCAATGTCT1130.10698224852071006No Hit
TAAGGGGGATTCATGAAGTGAAGCAGAAAGGGTCTGTAAGGAACATTCTAAA1130.10698224852071006No Hit
TTATTAGGGGCTCTTCCGATCTCTTGCCGGACCGTTGAGGAATTTCAGTCTG1130.10698224852071006No Hit
AGACTTGGGATATGTGGTTACTTAAGCATGGAAGCCATTGTGGGTATGTTTC1130.10698224852071006No Hit
ATGTAAGGGACACACATACACACACACGCACAAAGAGGACTGACTAAAGATG1130.10698224852071006No Hit
ATTAGTGGGCAGCCTGGCCAACACGGTGAAACCCCGTCTCTACTAAAAATAC1130.10698224852071006No Hit
CAAAGAGGGCCGCCTCGGCCTCCCAACGTGATGGGACTATAGACATGAGCCA1120.10603550295857989No Hit
CTAGATGGGAGACCGTGTTTGCATTGAACATCAACATGAATATTCTGATACT1120.10603550295857989No Hit
AAGTAGGGGACAAAAAAAGAAGCAGGATTGCCCTTAGGTTAGAGAATAGAAA1120.10603550295857989No Hit
AATGTAGGGATCTGTCGGTGGGGAACCCATTCTTCCAGCTTCTTCTAGTGTT1120.10603550295857989No Hit
TGCGTTGGGCACTCTACCAGTACCAGTTTCCTGTATTGGTCTGTTTTCATGC1120.10603550295857989No Hit
ACACAAGGGGGGCCATTTCTGCTTAGATGGTTGGCAGGGTCGCGCTCAGCTT1120.10603550295857989No Hit
TATAGTGGGGTAAAAAAGGTTCACTAAGCCAAACACCTTAGAAAATAGCCTC1110.1050887573964497No Hit
TAAGATGGGGATCTTGGAGAGGGTACTCTCTCCTCCTAGAATGCTGCCCTTG1110.1050887573964497No Hit
CACGCAGGGATTATGGGCATCGAGAACATCTGCGAGCTGGCCGCGCGCCTGC1110.1050887573964497No Hit
CTAGGTGGGAGTAAACCCATTGGAAGTCAAATGCATTTAATACCCCTGACTG1110.1050887573964497No Hit
TGTTCGGGGAGAGCCAGGCAATTTGTTTTATCAAGCCCTCCAGGTGACTCTG1100.10414201183431952No Hit
GTTTATGGGAAGCAAATTATTTTGAGACCTTTTGCTGGTAGACAAAAACTGA1100.10414201183431952No Hit
ACTTTCGGGGTCCATGGAGTCAGGGCAGCCGGCTCGACGGATTGCCATGGCG1100.10414201183431952No Hit
GCGATTGGGATGGTTATCTTGTTTCTGTTAGAAACAAAGTTGTATATAGAAA1100.10414201183431952No Hit
TACGGATGGGACCCCTGGGATGCAAGGACGGTTCAACATATGCAAATTAATA1100.10414201183431952No Hit
GCAACAGGGTCCTGTGTAGGCTTGGGTTATTGTGTATATTTGTGTCTTAAAA1090.10319526627218935No Hit
TCATGCGGGAAACCAGGCTTACCGTGAATTAAGATCCAAATGAAAGAGGAGT1090.10319526627218935No Hit
AAATTCGGGATTACTGATTTCAAATTTTTGACAAGCTGCTCATATCTTTAGG1090.10319526627218935No Hit
ATTGAGGGAGAAAATCCAGAAATTGTTCCAAGTGCATATGGAAAGTTAATAT1090.10319526627218935No Hit
AGCCAGGGGGAAGTTAAACAGTATGCTCCTGAATGACAAGTAGATCAATGAA1090.10319526627218935No Hit
AACGCTGGGGCTGGGGCTATTATCTCTATTTGGCAGATGAGGAAACCGAGGT1090.10319526627218935No Hit
ATTCTTGGGAAAAGGAGAGTTGCTGAGCCAAACAAGACCATGTGCATCATAA1090.10319526627218935No Hit
TTGGCTGGGGCTTGGGTTCAGGCAGATTCCCACTTTCACCTCTAGAGATTTA1080.10224852071005917No Hit
TTCCCAGGGACACCGTAGCGGCGCGCGAGCGGGCCGGGCGGGCGGCCGAGTT1080.10224852071005917No Hit
ATGCAGGGTTTTGATAAACTTTGCAAGAAAAGCCCTTACCTCTAATTGCATT1080.10224852071005917No Hit
TAACATGGGCTGGTGGGTGTGTAGTGGTATACCACTGTGATTATAATTTGTA1080.10224852071005917No Hit
TATACAGGGCATGCATTTTAAGTGTACATTTCAATTATTTTAGTAAATATGC1080.10224852071005917No Hit
TAATAAGGGAGATTGAAACCAGAACAGGGGGCTGAGCTCCAAACTGGAGAAG1080.10224852071005917No Hit
CTGCGTGGGAAAGAGCTGGGGCAGAGGCAGGAAGCAGGGGCCCTCCTGGCAG1080.10224852071005917No Hit
CTGCAGGGGGAGTGCCTCCCGGGCCCTCCACGGCCTGCCCCTTTCCCACAAG1080.10224852071005917No Hit
GTCATGGGGATATTCCTTTGAGCATTGCCGTTTTCTAGACAGACAATCCGAA1070.101301775147929No Hit
GTTTTGGGGAAGATTAGACTAAATGATTTTCACAAAGCACATTCAAGGTTTT1070.101301775147929No Hit
TTGCCAGGGTGCCCTTTTCCCTCCATGGATTCATTTCTATCTATTGCACTCT1070.101301775147929No Hit
GTCGAAGGGATTGTACTGAAAGTACCAGAAACTTTGGCTCTGCTTCTTAGCA1070.101301775147929No Hit
CTTCGGGGGGAAGACAGTGAGACCAAAGGATAAAGAGAGGCAGAAAGTGTTG1070.101301775147929No Hit
CTAAGTTCGGGGCACAAGGGCTGCTTAACACATTCCTATGCTACAAAAGACA1070.101301775147929No Hit
ACCTAGGGGCGATCTAGTCTCGGGAGTGCTGCGATGAACATATGGGTGCATG1070.101301775147929No Hit
GGGCGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1060.10035502958579882No Hit
ACGCCTCGGGACAAAACAGACTTTAAACCAACAAAGATCAAAAAAGACAAGG1060.10035502958579882No Hit
CATTGAGGGATTATGCTTGGTCTCAAAAAATATGTCTGAGAATATCATTGCA1060.10035502958579882No Hit
AGGAGTGGGCTGAGGACTTGAATTTACAGTGCATCAGATTTGTTGCAAATTT1060.10035502958579882No Hit
AGTTAAGGGGAGACCTGAACTCCTCCTTCAGGGAACTTAACAGTTTTCAGTG1060.10035502958579882No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)