FastQCFastQC Report
Sat 31 Dec 2022
SRR5847185.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847185.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104605
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT5220.49902012332106493RNA PCR Primer, Index 34 (100% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC5050.48276851010945937No Hit
AAAACTGGGATTAAGAAAAGACTAGAAAAGCAAACACAAAGCACACTGTGAT2070.1978872902824913No Hit
GCTGTTGGGGCACCAGAAGATGTCTGCATTACTCATTGCTAAAAATGTGTAG1720.16442808661153863No Hit
GAGGCGGGCGGATCACTTGATGTCAGGACTAGCCTGGCCAACATGGAGAAAC1620.1548683141341236No Hit
TACGCAGGGGCAGATTAGGATGGCCTAATGGTAAAACATCAGGCACATTCTA1620.1548683141341236No Hit
AGCACCGGGATTGGTTTTGGTAATCGATGTGGAAAACCCAAAGGACCAAGAG1610.15391233688638212No Hit
AGTCTTGGGGTTTAAGAGAGAAAGGCGGAAGAAATGAAATAGAAAAGCAAAA1570.15008842789541607No Hit
GGTTATGGGAATGAACCTAACCTCATTCTTTGTATCTTCAGTGTTGAATTGT1520.14530854165670856No Hit
AATACTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1500.14339658716122555No Hit
AGCCCTGAGCCAAATAGTAGAAAAGCTTGTGTTCTTTGATTCAACCCATTTT1480.14148463266574254No Hit
TCCTCAGGGATGTAGTAGATGGAACTTCCCCCAGAGCCCCAGGCCAATGGGG1440.13766072367477655No Hit
TTCTCTGGGGACCCACCACAAGGATGACCCTCCTCCTCCTGAAGATGATGAG1420.13574876917929354No Hit
CTCACACAGGGACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTA1420.13574876917929354No Hit
CGTTTGGGGAGATCAAAGCCAGGGAAAGCAATCAGAAGCCAGATTGCATTCT1420.13574876917929354No Hit
GCAACAGGGTCCTGTGTAGGCTTGGGTTATTGTGTATATTTGTGTCTTAAAA1400.1338368146838105No Hit
TTACCTGGGCAAAGGACATGATCTTGTTCCTTTTGATAGGTGCAGCAAACTA1400.1338368146838105No Hit
TGTGGGGCATTGAGGTTCATCAATGAATTTGTACATTTCTAGTTCCCTTTGG1380.13192486018832753No Hit
GCGACTGGGGGAAGACGACAGCAGTGAGGCGGACTCCCCGAGCTCCTCTGAG1380.13192486018832753No Hit
CTGCAATGGGGTTCAAGTACGTCTCAGGGTCTTCAGCCGAGAGGAGGGCAGC1380.13192486018832753No Hit
TAACATGGGGAAGAGACAGTGCTCTGCCATGATGACAGGGCACTGCTAGGGC1380.13192486018832753No Hit
GACCAATGGGATTCTGGTTCATTAAATTGTTACCAAAACATTTTTGAAAGTT1380.13192486018832753No Hit
GAACAAGGGCGATCTGCAAGTGGGGACCTCAGGTGATCCGCCCGCCTTGGCC1350.129056928445103No Hit
TTCAGCGGACTTCTTAAGCAGCATCCTCATCCTTCTTCCTCTTCAGCTCTTA1340.1281009511973615No Hit
GGTACGGGTGGTAGTCCATAGGTAGAATGTGTTTTCAAACTCTGAGGATGGG1340.1281009511973615No Hit
AACCTGGGTGACAGAGCGAGACTTCGTCTCAAAAACAAACAAACAAACAAAA1340.1281009511973615No Hit
AACGTAGGGTGAAATAATTAATGTTGGCAAATATGAGAACAACAAAGTCAGC1330.12714497394962No Hit
TCTCAGGGGTAAGAAATATAGTTTACTTTGTAACCTCGTGCATTTATATACA1320.1261889967018785No Hit
GCGTATGGGGATTTTGCTGGTCTTTGCATAACTGGCAGAGTGCAAGTTTGAT1320.1261889967018785No Hit
TTAGCGGGGATACGATGATAGTCAGCCTAAATGAAATCCCTGAAGCATTTTA1320.1261889967018785No Hit
TAACAGGGAGATTTTTGTATTGATTTGCAAAATGCAGATTATATTTGATAGG1320.1261889967018785No Hit
GCTTTGGGGAATGAAACTCCAGAAAGGACATGTATCACTGGCAGCAGAATTA1290.123321064958654No Hit
GAAGCTGGGATCCCCATCAAGCTACCAATGACTTTCTTCACAGAATTGGAAA1290.123321064958654No Hit
AACATGGGGCTAAATTTGTATTGTTAATTTCTAATGTATTTATTTTGAGATG1260.12045313321542947No Hit
TTTTGAGGGCTCCTAACTTTGTCTCCTATTATTCTCTCCTGGGCTTTGTATA1260.12045313321542947No Hit
ATCATAGGGTAGACGCCATGGCTGCATTGAATGATATACGGTATCAGAGACT1260.12045313321542947No Hit
TGTGCCTGGGGACAATAAATTCAACACTTTAGATGAAATGAGCAAAAATTAT1260.12045313321542947No Hit
CGTGGGGATGTGGGTCCACTCATATGCTGTTTTTTAGGAAATATGCTAGAAA1260.12045313321542947No Hit
AACGCTGGGGCTGGGGCTATTATCTCTATTTGGCAGATGAGGAAACCGAGGT1260.12045313321542947No Hit
GTACGGGGCAGAAGCACGGGCACAGGAGCATGTTAAAGGCCCTGAAAAGACC1250.11949715596768797No Hit
GTTTATGGGAAGCAAATTATTTTGAGACCTTTTGCTGGTAGACAAAAACTGA1250.11949715596768797No Hit
GCTGCTGGGACTCTCTACAGGACACATTTTACATTCAAATATACAAGTAGGT1250.11949715596768797No Hit
GTTGGGGGTGGTATAAAAAAATGAGTATAACCATAGGTTTTGAACAAATTTC1250.11949715596768797No Hit
GTCGAAGGGATTGTACTGAAAGTACCAGAAACTTTGGCTCTGCTTCTTAGCA1240.11854117871994646No Hit
CATAATGGGGCAGACATACAGAAAGCTCTGTGTGATCAATAAGGGAGTGTCT1220.11662922422446345No Hit
TGCGTTGGGCACTCTACCAGTACCAGTTTCCTGTATTGGTCTGTTTTCATGC1220.11662922422446345No Hit
GATAGTGGGGTCTCTGAGCTTTTCAGTCCAAATCTTTGCAAGGCTCAAAATG1220.11662922422446345No Hit
ACTTATGGGCCTCTCAGCCTGAGTAACTGGGACTACAGGTGTGTACCACTAC1210.11567324697672196No Hit
CTGCGTGGGAAAGAGCTGGGGCAGAGGCAGGAAGCAGGGGCCCTCCTGGCAG1210.11567324697672196No Hit
CAACAGGGGTTTTAACTACAGGATTTTATACTTTTAATATTATTTGTTATTC1200.11471726972898046No Hit
GAGTATGGGTAGGGGCTGACTTGTGTTGTGATTATTCCAATTGTGGGCTTTG1200.11471726972898046No Hit
CAACGGGGAGGAAAATACACGTATATTAAGTCAAATGTACATCTAAAAATTT1200.11471726972898046No Hit
CACACCGGGAAGCTTCTGTACTAAGTATATGTAAAGCTATTATATCTTGTCT1200.11471726972898046No Hit
TCATGCGGGAAACCAGGCTTACCGTGAATTAAGATCCAAATGAAAGAGGAGT1190.11376129248123895No Hit
TGCAATCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1190.11376129248123895No Hit
CTAAGTTCGGGGCACAAGGGCTGCTTAACACATTCCTATGCTACAAAAGACA1190.11376129248123895No Hit
CGTATTGGGAAGAAATTTAAACTCTACGTTTGGTTTTCACATACAGCAGCTC1190.11376129248123895No Hit
AGTCGGGGGATGGAATAGCTATACTTGTAGTTAAAACTGTTTTCTTATACGG1190.11376129248123895No Hit
TTTGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1180.11280531523349745No Hit
CAGTTAGGGATTCAAATTACTTGGAGTATTCTTTCTGAGAAGCATTCTGATA1180.11280531523349745No Hit
TTGCTAGGGGCTGCAACTTCCGGGAGGTGCTTGTGTGCCTGGTGCGGGAGCT1180.11280531523349745No Hit
TACCGAGGGACGCTCTCCGATCTGGCTACGGGAAGCACATGGCCTCCCATCT1180.11280531523349745No Hit
GTGGCGGGTCTGTTTGTTGGTTTGTTTGTTTCTTCCACGTAAGGAAAAGTAG1180.11280531523349745No Hit
CATAGAGGGAGCAGATGCTGAGGCAGTTCATTAAACCAGGCCTCAGCTTCAG1170.11184933798575594No Hit
GTTTTGGGGAAGATTAGACTAAATGATTTTCACAAAGCACATTCAAGGTTTT1170.11184933798575594No Hit
ACTTAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1170.11184933798575594No Hit
ATTTTTGGGGCATGTACTTTAGAAGGAATGTACAACATAATTGAAGAAATTT1170.11184933798575594No Hit
CGTCAGGGGAGATATGTATTTGCTCATTTTGGAAATTACAGAAAATTACAAA1170.11184933798575594No Hit
GTAGAGGGGGGTATATTTGGACACAGAAAAATTTTTATATTTTATAAAGGCA1160.11089336073801444No Hit
TACACCGGGTACATACATGACTGATTATATAAAAAGTTTTTACTCTGTCAAG1160.11089336073801444No Hit
GCATAGGGGACATCACAATAATATATAATTCAGTCTCTAGTTTCCCTAGAGT1160.11089336073801444No Hit
TTCGACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1160.11089336073801444No Hit
CTCGAAGGGGTTGGACAACTTAAAGTGTCAGAGTCCCTTGATTTAGGCACGA1160.11089336073801444No Hit
ATGTCAGGGGCCCACCCACATAATGGAGAGTAATCTGCTTTACTCAATGTCT1160.11089336073801444No Hit
TATAAAGGGACCTAGGGGAGAAAGAGCCATGTAAATATCTGTAATAAACTTG1160.11089336073801444No Hit
ATTCTTGGGAAAAGGAGAGTTGCTGAGCCAAACAAGACCATGTGCATCATAA1160.11089336073801444No Hit
CACGAAGGCTGAGGATCAGAACGCAGCAACCCATCTCATTCGCCACGCTGTG1150.10993738349027293No Hit
ACTCGCAGGGGGGGCTGGTGGAGGCGGGGTCGAGATGGCGGCGCCTTTGAGG1150.10993738349027293No Hit
AACATCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1150.10993738349027293No Hit
GTCATGGGGATATTCCTTTGAGCATTGCCGTTTTCTAGACAGACAATCCGAA1140.10898140624253141No Hit
CTAGATGGGAGACCGTGTTTGCATTGAACATCAACATGAATATTCTGATACT1140.10898140624253141No Hit
ACGAACGGGATGAAATTATGCATTACCACATTCAAACACAAATCCATTGTAA1140.10898140624253141No Hit
GAGCTTGGGATCTCTCATTTTCCCATTGTTTAATTTCTAAGTGGATGTTCAG1140.10898140624253141No Hit
TAACATGGGCTGGTGGGTGTGTAGTGGTATACCACTGTGATTATAATTTGTA1140.10898140624253141No Hit
CTTCTGGGGAGAGTTACCAAAGGCTTGTTTTTTCTTGAGCAGTTGAAAGGAG1140.10898140624253141No Hit
AATAATGGGGGTAAGGCTGTGGGCAAATAGGCAATCATATATTGCTGGCAGG1140.10898140624253141No Hit
ATTGTGTGGCGCAGCCGCAGCAAGAGTGAGAGGAGCAGAAAGCGAGGCAGCA1140.10898140624253141No Hit
TTGCCAGGGTGCCCTTTTCCCTCCATGGATTCATTTCTATCTATTGCACTCT1130.10802542899478991No Hit
CTCCGGAGGGATCTGTCGACGGGCATAATTTCCTTTTCAGATAGCTCATTAA1130.10802542899478991No Hit
TAAGGGGGATTCATGAAGTGAAGCAGAAAGGGTCTGTAAGGAACATTCTAAA1130.10802542899478991No Hit
GTGAATGGGGTAAGCTTTTGGGCTCCACAAAACCTGGATTTGCCTCAATCTC1130.10802542899478991No Hit
TGATGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1120.10706945174704843No Hit
CTCCTGGGAGAGAGGAATGAGAAGAAGCAGCAGATGGGCAAAGAGTACCGTG1120.10706945174704843No Hit
AAAGCCGGGCTAAGAATGATGTAACCTTTTGTCTGTGTTATCTGAACACTCT1120.10706945174704843No Hit
AAATTCGGGATTACTGATTTCAAATTTTTGACAAGCTGCTCATATCTTTAGG1120.10706945174704843No Hit
GATACAGGGTAAATTTTACCTTAATATAGCTGATAAAAAATGCTCAACAAAT1120.10706945174704843No Hit
ATTGCAGGGTTACTTTCATGGTTTCAACCATTATTTTATGTCAAATAACTCC1120.10706945174704843No Hit
GTCATGGGGATGGGAGTTCCACAAAGAGAAGACAGAAAAATGAATGAAGAAT1120.10706945174704843No Hit
CTTAGTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1110.10611347449930693No Hit
AAACACGGGAAGGAGACTACAAGAAACCAGTTTTCTTTTATTATTAAGTGAG1110.10611347449930693No Hit
CATATAGGGGGGCTGGCAGTGCGGGCAGAGCCCGGCTGAGAGGGGCGGCCCT1110.10611347449930693No Hit
TTATTAGGGGCTCTTCCGATCTCTTGCCGGACCGTTGAGGAATTTCAGTCTG1110.10611347449930693No Hit
AGGAGTGGGCTGAGGACTTGAATTTACAGTGCATCAGATTTGTTGCAAATTT1110.10611347449930693No Hit
ACATTTGGGTGGAATAGGGCTTACTCACCCCTTCATGGAGGCTCGGGCAGCT1100.10515749725156541No Hit
GAAGACGGGTGGTTTTTAAAGACTCACCTTTTTAAATGGTACTTGGAGCTCC1100.10515749725156541No Hit
ATGCGCGGGCTCCTTCTGTCGCCCAGGCTGAAGTGCAGTGCTGTGATCTCGG1100.10515749725156541No Hit
TAGTCTTGGGAGCGCCACATCTCCTGAGATTATTTGTACGAATTGGAGCAAT1100.10515749725156541No Hit
GAAACTGGGATGTTATAAACTTTTTAATTTAAAAAAGTTTTTAACTTCTTTG1100.10515749725156541No Hit
TCACTAGGGGACTACATTTATCTGCAATTTTTAAAATTTTCCATATCTTTGT1100.10515749725156541No Hit
CATTCAGGGATGTAAATGTGATTTGTTCTGATTGAAATAATCTCCCTATTTT1100.10515749725156541No Hit
ACTAAAGGGACAATAGATAATCTTTGGAATGTATGTTTTAAATGCTACTCAT1090.1042015200038239No Hit
AGGAATGGGACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCG1080.1032455427560824No Hit
GTAATTGGGCAGAATGCACAGCCAAGTAGTAAATAAATGAGAGAGAGTGACC1080.1032455427560824No Hit
GTAGACGGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGG1080.1032455427560824No Hit
TCAGCCGGGGTTAGATCACATGGGAGGACAGCATCTTCTTCCCTGCCAATTT1080.1032455427560824No Hit
ATGCAGGGTTTTGATAAACTTTGCAAGAAAAGCCCTTACCTCTAATTGCATT1070.1022895655083409No Hit
ACCTAGGGGCGATCTAGTCTCGGGAGTGCTGCGATGAACATATGGGTGCATG1070.1022895655083409No Hit
ACAGAGGGCACCAGGACTTTAATCTTATGCTTAAAATGCCAGATGTTGTTCG1060.10133358826059939No Hit
TCACAGGGCAATACTAGCATACGTTTTTCTTAATTGTTATCAGCATTGACCA1060.10133358826059939No Hit
TTATGCAGGGGGAATTAAAAAATATGGTCCCTTTGTTGCAGACTTTGCTGAT1060.10133358826059939No Hit
AACAATGGGACTCGTTTCTAGGGTTTTTTATTTGCAGTTTAGGAACTATTAG1060.10133358826059939No Hit
TGTCAAGGGCTGGGCTGTAATTAGTGTATGCCTGGAAGTGCAGACCTTTTCA1060.10133358826059939No Hit
ACACAAGGGGGGCCATTTCTGCTTAGATGGTTGGCAGGGTCGCGCTCAGCTT1060.10133358826059939No Hit
GATCTAGGGGTCTTCCTGCCCCTTGATGCTCAACTAAGCATCTGTTCCCTAG1060.10133358826059939No Hit
CTAGGTGGGAGTAAACCCATTGGAAGTCAAATGCATTTAATACCCCTGACTG1060.10133358826059939No Hit
GACGCAGGGCAGACACACCTTATGAATAAGACTTTATTACATAATTATTTCT1060.10133358826059939No Hit
ATGTAAGGGACACACATACACACACACGCACAAAGAGGACTGACTAAAGATG1060.10133358826059939No Hit
TAGAACGGGACTCATTACTGCAAATTGATTGTCTTTCCCCCCTCCCTCCAGT1050.10037761101285789No Hit
ACTTACGGGTTAAAAGCATCCGTGGACTATATCCGAAAGTTGCAACGAGAAC1050.10037761101285789No Hit
CCACCACGGGGGGTGCTTCTCAGTGGGAAGGTGTAAGGAGGGGCTTGCTAAG1050.10037761101285789No Hit
GCACGGGGGTGGTTCTTTTTTAGAACAAGTTTTCAAAGCATAAAAAGAGGTT1050.10037761101285789No Hit
TATAGTGGGGTAAAAAAGGTTCACTAAGCCAAACACCTTAGAAAATAGCCTC1050.10037761101285789No Hit
GCGCGCGGATTCCATTTTTAAGAAGAGGTAATGTGTGTGTGTGTGTGTGTTT1050.10037761101285789No Hit
TACGGATGGGACCCCTGGGATGCAAGGACGGTTCAACATATGCAAATTAATA1050.10037761101285789No Hit
TAACTCGGGGAGACAGCGCCGGGAAGATGAGCACGGAAGGTGGTGGCCGTCG1050.10037761101285789No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)