FastQCFastQC Report
Sat 31 Dec 2022
SRR5847178.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847178.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240372
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT7940.33032133526367463RNA PCR Primer, Index 34 (100% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC7920.3294892915980231No Hit
GAAACGGGGCGGGAGCAGCTGCTCCGAGACGAGTGTTTTGGGAAGAGCTGTG3420.14227946682641907No Hit
GCGTCGGGGAGGCGGATCTAGGATTACACAGGCTGCAGGCAGATCCACGATT3110.12938279000881966No Hit
CAACAAGGGGTTCCTACCTCTGTGCTTTTAATGAAAACATTTCTGCTGTTTA3100.1289667681759939No Hit
TCTGCAGGGTGTGAATTGTAGGGACCAGAGAACATTATTTTGTTGTTCTAGT2830.11773417868969763No Hit
GCAAGCTGGGGTATACAAGTGAGATTAACTAACAACAGCTAAAGTGAAAGAT2740.11398998219426557No Hit
AACTGAGGGACTGGAGGTCAGGCAGCTGCTGGCCCCGCGGCATCTGAGGGGA2680.11149385119731084No Hit
TCTGTGAGGGGCAAGATCACACCACTGCACTCCAGCCTGGGCAACAGAGCGA2670.11107782936448504No Hit
TAAATGGGGGCACTAATCCATTCATGAGCACAGAGCCCTTATGATTTCATCA2620.10899772020035613No Hit
TAACGTGGGCGATCTCAACCTGGGAGGATGTTTAGCGGCATCCCTAGATTCT2610.10858169836753032No Hit
TTGCATGGGAGAGATAACACTGGGAACATATGAAACTTTGTCAGCATCAGAT2540.10566954553774982No Hit
TTCACGGGTATTATCCAAGAAGAGATCCTGATCAAAACCCCAATAAAGGGTT2450.10192534904231774No Hit
AAGAACGGGATGAGCTTTATTTTTATCTAAACCATTGGTGGACATTAGCAGG2440.10150932720949196No Hit
GACATTGGGAAAGGAACTGAAGATGGAAAATATGACTAATAAGTTATTGCAG2430.10109330537666616No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)