FastQCFastQC Report
Sat 31 Dec 2022
SRR5847177.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847177.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242336
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC8180.3375478674237422No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT7430.30659910207315466RNA PCR Primer, Index 34 (100% over 21bp)
GCGTCGGGGAGGCGGATCTAGGATTACACAGGCTGCAGGCAGATCCACGATT3270.134936616928562No Hit
GAAACGGGGCGGGAGCAGCTGCTCCGAGACGAGTGTTTTGGGAAGAGCTGTG3130.12915951406311899No Hit
CAACAAGGGGTTCCTACCTCTGTGCTTTTAATGAAAACATTTCTGCTGTTTA2800.11554205730886043No Hit
TCTGTGAGGGGCAAGATCACACCACTGCACTCCAGCCTGGGCAACAGAGCGA2790.11512940710418593No Hit
GAGTTGGGGAAATACACAATGTCGTGCAACTGAAACCACTGAGATAGAAAAA2730.11265350587613891No Hit
GACATTGGGAAAGGAACTGAAGATGGAAAATATGACTAATAAGTTATTGCAG2630.1085270038293939No Hit
TTCACGGGTATTATCCAAGAAGAGATCCTGATCAAAACCCCAATAAAGGGTT2620.10811435362471938No Hit
TTGCATGGGAGAGATAACACTGGGAACATATGAAACTTTGTCAGCATCAGAT2590.10687640301069588No Hit
TGCTAAGGGGTCTTCCCTGTAAGTTTAAGACGTTTCCAAGGAAAAGCTTCAG2570.10605110260134688No Hit
TGATACGGGACTTGGGAACAGAAAGCCAGATCTTTATTTCCCGCACATATGA2470.10192460055460188No Hit
GCTTCAGGGATCTGCCTTCTGGCTCATTTAATGATGACTGCAATCTGTACCT2450.10109930014525288No Hit
AAGAACGGGATGAGCTTTATTTTTATCTAAACCATTGGTGGACATTAGCAGG2440.10068664994057838No Hit
TCTGCAGGGTGTGAATTGTAGGGACCAGAGAACATTATTTTGTTGTTCTAGT2440.10068664994057838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)