FastQCFastQC Report
Sat 31 Dec 2022
SRR5847176.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847176.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237415
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC7940.334435482172567No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT7670.32306299096518754RNA PCR Primer, Index 34 (100% over 21bp)
GCGTCGGGGAGGCGGATCTAGGATTACACAGGCTGCAGGCAGATCCACGATT3100.1305730471958385No Hit
GAAACGGGGCGGGAGCAGCTGCTCCGAGACGAGTGTTTTGGGAAGAGCTGTG3090.1301518438177874No Hit
TAAATGGGGGCACTAATCCATTCATGAGCACAGAGCCCTTATGATTTCATCA2620.11035528504938609No Hit
TAACGTGGGCGATCTCAACCTGGGAGGATGTTTAGCGGCATCCCTAGATTCT2540.10698565802497736No Hit
TAAAGTGGGGGCCGTACGGCGGAGGCGGGGTTTCAGTGGCTTCTGGTGCTCT2520.1061432512688752No Hit
GCAAGCTGGGGTATACAAGTGAGATTAACTAACAACAGCTAAAGTGAAAGAT2460.10361603100056863No Hit
AGTCAAGGGGTAATCTGGATTTATACCTTTTAAAAACCATTTTGACCAGTTT2430.10235242086641536No Hit
TCTATCTAGGGTCTTCCAGTTCCTTTCGAAATCATGAAAGAAGGCATGCGGA2420.10193121748836426No Hit
GAACGCAGGCACATACTTCCTATTCTACACCCTAGTAGGCTCCCTTCCCCTA2390.10066760735421099No Hit
GCTTCAGGGATCTGCCTTCTGGCTCATTTAATGATGACTGCAATCTGTACCT2390.10066760735421099No Hit
AACTGAGGGACTGGAGGTCAGGCAGCTGCTGGCCCCGCGGCATCTGAGGGGA2380.1002464039761599No Hit
TCTGTGAGGGGCAAGATCACACCACTGCACTCCAGCCTGGGCAACAGAGCGA2380.1002464039761599No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)