FastQCFastQC Report
Sat 31 Dec 2022
SRR5847175.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847175.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120713
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC5740.4755080231623769No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT4800.3976373712856114RNA PCR Primer, Index 34 (100% over 21bp)
TGCATAGGGGGGACAACTAAGAGATCATTTGCTTCTTTCGGCGTAAGAAGGA1340.11100709948389982No Hit
TGTCCTGGGATGTCTCAGCAGTTCTTACCTTTATAAGAATTCTAACCATTGA1310.10852186591336477No Hit
AATCTGGGGGGCCGGGCCTGAGGGCGTGTATCCGCGGCCGTAGCAGCCGGGC1290.10686504353300803No Hit
TTTTCAGGGAAGCAGCAACAATATATCATTTTACATTTATCAGCTAAGCAAA1270.10520822115265133No Hit
ATCATTGGGCTGCCACTAGGAAAACAAAAGCACTAGCTTTTGTTCTCAGGCT1250.10355139877229462No Hit
TTCACCGGGTGTATTGGGGGAACCAGAGTGACTTCATCTTGAGTGAGGGTTA1210.10023775401158119No Hit
GTGCATGGGGCATTATAAACTTTTTCCATTCATAAATACATAAGTGAACCAA1210.10023775401158119No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)