FastQCFastQC Report
Sat 31 Dec 2022
SRR5847174.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847174.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120990
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC5490.4537565088023804No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT4000.33060583519299114RNA PCR Primer, Index 34 (100% over 21bp)
TGTCCTGGGATGTCTCAGCAGTTCTTACCTTTATAAGAATTCTAACCATTGA1550.12810976113728406No Hit
CGTAAAGGGAAGAAATGAACCGAATGCATGAAAGCATGAAATTGTTTGACAG1390.11488552772956442No Hit
TGCATAGGGGGGACAACTAAGAGATCATTTGCTTCTTTCGGCGTAAGAAGGA1310.1082734110257046No Hit
AATCTGGGGGGCCGGGCCTGAGGGCGTGTATCCGCGGCCGTAGCAGCCGGGC1270.10496735267377469No Hit
GCTTGGGAATCCAATCTGGGAAGATTGCTTGTAGGAGGAGACCAAAAGACAA1260.10414083808579222No Hit
AAACATGGGACTTCACCTCTGAGCCTCCGATGCTCTGTGAAATGGAAGGAAT1240.10248780890982725No Hit
AGTGCACAGCAAGAGCGTATCAATGACCGCTATTGTCACTCTTTAATTTTCA1240.10248780890982725No Hit
GACAAAGGGCATCTCTCAGTGGCACAAGGCTTAAGAGGGAAAAATGGGAAGG1230.10166129432184479No Hit
TTGCTAGGGAGGCAGTAAGAATGTGGAGAAACAGGAACCCTCATACATTACT1210.10000826514587982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)