FastQCFastQC Report
Sat 31 Dec 2022
SRR5847173.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847173.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119016
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC5340.4486791691873362No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT4340.3646568528601196RNA PCR Primer, Index 34 (100% over 21bp)
TGCATAGGGGGGACAACTAAGAGATCATTTGCTTCTTTCGGCGTAAGAAGGA1380.11595079653155878No Hit
CATTTGCCGGGCGGGCAGTGGACCATGTGCAGCGCAGCCTGAGCTGCTGTGG1370.11511057336828662No Hit
TTTTCAGGGAAGCAGCAACAATATATCATTTTACATTTATCAGCTAAGCAAA1330.11174968071519795No Hit
GTATTCGGGATGGATCAGTGGTTTCTAACGTTGGTTTTGTGAAATGTTATGA1310.11006923438865364No Hit
GTGGCTGGGGCCGATCTTTAGGCGGGGACAGCTCTCTGCCAGATTAAGGGAG1280.10754856489883713No Hit
GACAAAGGGCATCTCTCAGTGGCACAAGGCTTAAGAGGGAAAAATGGGAAGG1210.10166700275593198No Hit
GAATCAGGGGAGACAGGGTTTCACCATGTTGACCAGGCTCGTCTCGAACTCC1200.1008267795926598No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)