FastQCFastQC Report
Sat 31 Dec 2022
SRR5847172.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847172.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159090
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC3630.2281727324156138No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT3390.2130869319253253RNA PCR Primer, Index 34 (100% over 21bp)
ACATGCGGGGTGTTTCTCTAAGTTTCTGAAAATTATGACATCACATTCTTAT1730.10874347853416305No Hit
TCTGAGGGGACGAAAAAAACTGGAAACTAATCCGGTAAACATTTAAACTTGA1710.10748632849330567No Hit
GTACTTGGGCATTTATACTTTATTTTCAGAATACCAGTGGTTTTCTTAGCTC1700.106857753472877No Hit
TATTCCGGGAATTTGAACTTTCTCTAGAGCCAATTTTTGCATGTGTGTGCAG1690.10622917845244831No Hit
TAGCCAGGGATGAATGTATCTTTCTAAAGGACTGACGTTCAATCAAATATCT1680.10560060343201962No Hit
CACATCGGGGTTGTTTAATGTTTATACACTGCTGGTGGGAATGTAAATTAGT1670.10497202841159092No Hit
GTCTTTGGGGAGGAGATGCCTGAGGACACAGAAGACTTAGATCACTATGAAA1660.10434345339116223No Hit
TTGGAAGGGGGCCCTGCGGGAGCAATCCGCTTCCTTCAGAGGGTCTGTGGGT1660.10434345339116223No Hit
CTGAAAGGGAAGTCACACAGTTATGATTGCACAGTAGATCTATTGGAGTTTC1660.10434345339116223No Hit
CCAGCTGGGAAAGAAAACAGGTTTACTACAGAATGGGAAAAAATATTTGCAA1660.10434345339116223No Hit
CACTGTGGGGAGAGGCAGGTGTCAGAGGCAGAGGAAAATGGGAAATTGGATA1650.10371487837073355No Hit
TACAAGGGGGAGGCGCTTAGGGGCTGAGGCGCGATGGCAGGTGTCGGGGCTG1650.10371487837073355No Hit
TAACTAGGGTGGTAATGTCTCCTTTCATCACTGATTTTAATAATTTGAGTGT1640.10308630335030486No Hit
GCAATAGGGGCGACATCCAGAAATGAGCTGATGTCAATGCTATGGACTGCAC1630.10245772832987617No Hit
ATCAATGGGCAAAAGATGGATCTCACTTCACTCTGAAAATGTGCCTTTTTTT1620.10182915330944747No Hit
TTGGTAGGGCGACATTGATATGACAATTCAAAGATAACTTAAGCAGAATGTG1610.10120057828901878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)