FastQCFastQC Report
Sat 31 Dec 2022
SRR5847171.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847171.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158261
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC3440.2173624582177542No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT2940.1857690776628481RNA PCR Primer, Index 34 (100% over 21bp)
AGATAGGGGCTGACCACGGGTAGGCTTAGGTTTATGTGTGTGTATGTGTCTT1830.11563177283095646No Hit
TACACCGGGACTCTAATTTTCATGTAATCAGAAATGTTACCTTTGCGATAAG1780.11247243477546585No Hit
ATCCATGGGGAAGAACAGACCCCTAAAAAGGGAAAGTGTTCCTTTCAGTTTT1760.11120869955326959No Hit
TCTGAGGGGACGAAAAAAACTGGAAACTAATCCGGTAAACATTTAAACTTGA1700.10741749388668086No Hit
GTCTTTGGGGAGGAGATGCCTGAGGACACAGAAGACTTAGATCACTATGAAA1690.10678562627558273No Hit
CTGCCTGGGTATTTAATTGAAGGTACACATTCCATCCGTGGAAGAAATTGCA1660.10489002344228836No Hit
GCCTAGGGGTAAGTATCTTATTTCTGATTGCAAGTTGCATATTTTTAAATGT1660.10489002344228836No Hit
AGGCTTGGGTGTAGTAAAAAGCTGTAATAACAAAACAGCAGGATTTTCATAG1620.10236255299789587No Hit
AGCACGGGGGGGTTATAGGTGTGAGCCACTGTGCCATCCCAGTTTCCAGAAT1620.10236255299789587No Hit
CAGATCAGGGATTATTAAGTGTTATCCTGCATTTTTTGACTGGCAAACTCAA1600.10109881777569964No Hit
GACATGGGGGCTTTCTTCCTTTTGGTGCGAGCTTGCTGTGGTTTTTGCTCTG1600.10109881777569964No Hit
GCTTCTCGGGGGTGACACATGAGACTCACAGTTGGAGTTCTCCAGCTCTGGA1590.10046695016460153No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)