FastQCFastQC Report
Sat 31 Dec 2022
SRR5847170.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847170.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159286
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC3600.22600856321333954No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT2940.18457365995756062RNA PCR Primer, Index 34 (100% over 21bp)
AGATAGGGGCTGACCACGGGTAGGCTTAGGTTTATGTGTGTGTATGTGTCTT1930.12116570194492926No Hit
TCTGAGGGGACGAAAAAAACTGGAAACTAATCCGGTAAACATTTAAACTTGA1770.11112087691322527No Hit
GCATTGGGGCAGTCTTTCAATATGTGGTTTTCAAAAAATTGTTGAAGACGTG1730.10860967065529926No Hit
CGCAATGGGAAATATTTTATTGCTGATTTTTTTTACTGATTGCATGGTAAAG1720.10798186909081776No Hit
TGAAAAGGGGCCGGCTGCAGGGATGGCGGGGAGGGGTAAGCTCATCGCAGTG1660.10421505970392878No Hit
CTGCCTGGGTATTTAATTGAAGGTACACATTCCATCCGTGGAAGAAATTGCA1650.10358725813944729No Hit
GTTTACGGGGCAATAAGGGGTTGTGTTGTTTATAGAGATTTTTTAAAATAAT1650.10358725813944729No Hit
GACATGGGGGCTTTCTTCCTTTTGGTGCGAGCTTGCTGTGGTTTTTGCTCTG1620.1017038534460028No Hit
GTACTTGGGCATTTATACTTTATTTTCAGAATACCAGTGGTTTTCTTAGCTC1620.1017038534460028No Hit
CGTAACGGGTACAATGAACTCAAACAAATTTACAAGAAAAAAACAAACAACC1610.10107605188152129No Hit
TTGGTAGGGCGACATTGATATGACAATTCAAAGATAACTTAAGCAGAATGTG1600.10044825031703979No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)