FastQCFastQC Report
Sat 31 Dec 2022
SRR5847162.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847162.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217269
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC5660.26050656099121366No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT4820.22184480989004413RNA PCR Primer, Index 34 (100% over 21bp)
CAATCAGGGATAATTAGTTTACATACATACTCCTTAAACAGATAAATACATT2410.11092240494502206No Hit
CAAACAGGGTCTTCTGTCTGTTATTGTCATTACTTTGTCTAAGGACAGGACA2290.10539929764485499No Hit
GTATATGGGAAAGGAAAAAAGCAAAAACATCCTTGACTCATGTATTGAAGGG2190.1007967082280491No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)