FastQCFastQC Report
Sat 31 Dec 2022
SRR5847157.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847157.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185380
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC7100.3829970870644082No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT4760.25676987808825114RNA PCR Primer, Index 34 (100% over 21bp)
AACACGGGGATTCAGGTAACTGAAGATTTTGTGTCTCCAGAACATCTTGTAG2290.12353004639119645No Hit
AACGCGGGTGAACATGCCGTAGTGCCTTTATGGCCAGTTTGAGTCCTGCCTA1960.10572877333045637No Hit
CAGTACAGGAGCCCAAAGTGATTTTTCAGAAACATAAATCAGATTCCATCAC1940.1046499082964721No Hit
GAGGCTGGGGGTCTGGAGAACATTTTAAAGTAAAATCAAGTAGATTCTCTGA1930.10411047577947999No Hit
GTATACGGGACAAAAGAGGCAATTCAGGCAATGGATATGTCACATCATTTTT1920.10357104326248787No Hit
TGAAAAGGGAAGAATGTAAACTTTGAATTGGTTTGCATTTTAACAATTAGGA1920.10357104326248787No Hit
GCGTTGGGAATTTCAAAGGTCGAACCTCACACAAACCCCCTCTTTCCTTTCT1910.10303161074549573No Hit
TCTAAAGGGATGACTCCTTAGCAAAAATCAAATAACCCAATTATAAATGAGC1900.10249217822850361No Hit
AACTGCGGGTGGTTTATTTTGATAAGTCTGTGGCTTTGAGACTTAGTTTTAT1870.10087388067752724No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)