FastQCFastQC Report
Sat 31 Dec 2022
SRR5847156.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847156.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185948
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC6790.36515585002258694No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT4460.23985200163486567RNA PCR Primer, Index 34 (100% over 21bp)
GACAGGGGGAAGCATCATGAGCCTTCTTCCTGTTGCTCCAACAATTCTCTTC1950.10486802762062512No Hit
CACAGGGGGATCCTAAGGTAATATAATATAATTTTAGTGCATTTCTCCTCAT1930.10379245810656743No Hit
AACTGCGGGTGGTTTATTTTGATAAGTCTGTGGCTTTGAGACTTAGTTTTAT1930.10379245810656743No Hit
TGAAAAGGGAAGAATGTAAACTTTGAATTGGTTTGCATTTTAACAATTAGGA1920.10325467334953858No Hit
CTACACGGGTCATCATTTGCACCAGGTAGGTGACTTAACCTAAAGAGAAAAT1900.10217910383548089No Hit
AACGCGGGTGAACATGCCGTAGTGCCTTTATGGCCAGTTTGAGTCCTGCCTA1900.10217910383548089No Hit
CAGTACAGGAGCCCAAAGTGATTTTTCAGAAACATAAATCAGATTCCATCAC1900.10217910383548089No Hit
GAGTAAGGGCTGAGAATTCAAGCAATGCAGGCCAGGCGTGGTGGCTCACGCC1890.10164131907845204No Hit
TCTAAAGGGATGACTCCTTAGCAAAAATCAAATAACCCAATTATAAATGAGC1890.10164131907845204No Hit
GGCAAGAAGGGATTCGGCAAATATTTATTGAGCATTTACAACATGCCAGGCA1890.10164131907845204No Hit
CTACTTGGGGAGGTGTCTGCCTTTGTGCTTAATTGAATTGTCTTAAGAAACT1860.1000279648073655No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)