FastQCFastQC Report
Sat 31 Dec 2022
SRR5847155.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847155.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183048
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC5960.3255976574450417No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT4890.26714304444735804RNA PCR Primer, Index 34 (100% over 21bp)
GCGTTGGGAATTTCAAAGGTCGAACCTCACACAAACCCCCTCTTTCCTTTCT2050.11199248284602945No Hit
CATAATGGGAGCTGCTAAAATTTGTTCACAGAACATTGTATTTTAACTTACC1940.10598313010794984No Hit
CAGTACAGGAGCCCAAAGTGATTTTTCAGAAACATAAATCAGATTCCATCAC1850.10106638695861195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)