FastQCFastQC Report
Sat 31 Dec 2022
SRR5847154.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847154.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82597
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC3380.4092158310834534No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT2830.3426274562030098RNA PCR Primer, Index 34 (100% over 21bp)
CAGGCTGGGCTGCCCGCACCTGCTGTTCATGGTTCTAATAGGTATTTCAGGA1340.16223349516326258No Hit
GCGCACGGGATATGTCAATATTTAGAACATTGCATAACTCACTGAACCAATA1290.1561800065377677No Hit
ATAACCGGGCACCAAACTGGAGGATGCCTATGTATTCCCTGGGGATGGCGCA1270.15375861108756975No Hit
AATAGCGGGGACAAACCCATCATAAATTGAGGAGTGTATTTTATATGTATCA1230.14891582018717386No Hit
GGTTTAGGGACATTAACTCCTTACTCTAAAAACCTTAAATCTATTTTATCAT1160.14044093611148106No Hit
ACGCGCGGGGGTTTTGAGTCATCCAGTCCTGGGCACTGAGGTCTGTTAGATG1110.1343874474859862No Hit
TTCACGGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1080.13075535431068924No Hit
TTTAACGGGACGGGAAAGACTAAAAAATGACATCCAAAAATGAGCAAAAATT1070.12954465658559028No Hit
GCTTGTTGTCCTGAATTTTTTACCCCATTAGCAGTATCACTGAGTTGCAAAT1060.1283339588604913No Hit
AGTAACGGGATATCAAATGCAAACACAGCACTTTCTCAGACTCACTTATAAG1050.12712326113539232No Hit
TTACAAGGGTAGTGGAGTGAAAATGCCAAATCCTATGGATTTCTCCTTAACC1050.12712326113539232No Hit
CCACTTGGGTAACACAATTCAGTGGTACTTGGAATCCTGTTTTAATTTGCAC1000.12106977250989745No Hit
AGCCAGGATGGAAACGCGTGTCCCAAGTGTCCGGAGCAGGCGGCAGAGGCCT1000.12106977250989745No Hit
AGGCCCAGGTCGGGCTTCCCACCTCAGCCAAGCCTGCCCCTTTGCAGGGCCC1000.12106977250989745No Hit
GATAACGGGACCTATTTCCAAGCCAGTATAAGAAACTATTAACTTTGGGTCC1000.12106977250989745No Hit
ACTTAAGGGAAACCAATTAAAACATACTTTTACTCTCAGATATACAGGTATT1000.12106977250989745No Hit
ATCAAAGGGTGTCAATAGAACCTGAAAAAAGATTTTTTTAGTTGCCTCTAGT990.11985907478479847No Hit
CGACTTGGGTTTGAAGAACTAGGACAGAGGCTGCAGCATTATGCCAAGATAG980.1186483770596995No Hit
ATGCTTGGGATCTCAGCTCACCGCAACCTCCGCCTCCCAGGTTCAAGCGATT980.1186483770596995No Hit
ATTAAAGGGAAATGACCACTGGGTAAATAACGAAATTAAGGCAGAAATAAAT980.1186483770596995No Hit
AGCCTTGGGATGTGCCAGCAAGCCAGAGGTGACTGTGCGGCTGAATGTCCAT970.11743767933460054No Hit
ATGACTGGGATGCCTTGTAGGTAAGCAAAAATAATGAATTGTTTTAATATCA970.11743767933460054No Hit
TTATATGGGTCTGAGAGAGGGATTATAAACCATTGCCTAGGTATTCTCAAAT970.11743767933460054No Hit
GCGATAGGGACACATCTATCACCTCCCCTGCTATATATTAGACCCTGAGATC970.11743767933460054No Hit
TCATGCGGGATTGAACTTTAGTTTCAAATCTTCACTCAACTGTACTTCCCCT970.11743767933460054No Hit
AATACGGGGTCTGCAAATGGGGAGTTACAGGGAACTTGAGGAGAAAAGCAAA960.11622698160950157No Hit
ATTTTAGGGAGATGGGATCTAATTAAACTGGAGAGTCTGCATGGAGAAGGAC960.11622698160950157No Hit
CTAGCAGGGATTTGCTTGGATGTCCAGGTATAGGTGAATGCTTAGGAATATA960.11622698160950157No Hit
GTTCTAGGGAGTGAGCCAGGATTTGATTTCACTGCCTTATATTCTATTTTTA950.11501628388440259No Hit
AAGAAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC950.11501628388440259No Hit
AAAAATGGGAACAGAAAAAAATTCTGTAAGCTTATATGCCAAAAACTTACAG950.11501628388440259No Hit
CGACTCGGGATATTATGTTCGTTTTGTGAATGAATGAAATTTCAGTTCACAT950.11501628388440259No Hit
GGCTAAGGGAGGAGCCGACTCTTTGATATGCCACCAGCGAACTCTCAGAAAT950.11501628388440259No Hit
GCTCGGGACCTGGAATTTAAAATTCAGTAGACAAATTAATTGGTCCTCTGCA940.11380558615930361No Hit
TTGGGTGGGTTGTCATCTTCAGAGTATATAACTGCTGGGATTCAGTGCGTCA930.11259488843420463No Hit
GTAAGCGGGCTTCGCTGGCCATTTAGGAGCTCTGCTCAAAGCCAGACGTATC920.11138419070910566No Hit
GATTAAGGGTCACTATTAATGAAATGTGTAAATTCTCCAGAGTACCTGATTT910.11017349298400668No Hit
TGATATGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG910.11017349298400668No Hit
GGAATAGGGAAGTGCCAGCAGCTGCACAGATACTTCTCTATTCAGCCAATTC900.1089627952589077No Hit
AGGTCCGGGCTGGCGGTGGGTAAACTCAAGCTGGGAACTGGGGAGTGGGATC900.1089627952589077No Hit
TATCCAGGGTCCATTCCTGTGCATTGCCTTTTTTCTGGCTGTCACGCATTCT900.1089627952589077No Hit
TGTGGTGGGCAGAGGATATGTTGCAAAGAGATTGATTAGATTTTCTTTTCTC900.1089627952589077No Hit
TCGATCGGGGTACACCCAAGCCAATGGACTGGATTCCTATTTTAACTAGAAA890.10775209753380874No Hit
TTGGATGGGTGGTTTTAGATGATAACCACAGGTCTACAAGAGCGTTTTTAGT890.10775209753380874No Hit
TTGAGTGGGGATTCAGATCTAACCCTTCTATGAGTTCATAAAATGGTAGAAT890.10775209753380874No Hit
AGACTAGGGACTTGATGCTTGTGTTAGAATTGTATTTCTCTTCTTCAGATGG890.10775209753380874No Hit
ACGGCAGGGTGCGGGGACGCGCTCGCGGCGGGACGCGCCGCGGTGGGGGATG880.10654139980870976No Hit
GGTAATGGGAATGTAAGAAAACTTGAACTAGCTTTTAAATTTTTCAATGAAT880.10654139980870976No Hit
TACAACGGGTGTTTTAACAAGGTTTCTGGTGAAGACAAGCTATCAGTCTCTT880.10654139980870976No Hit
AGAAAAGGGAGTTACATGAGGCTTTTATGAAATTCTGTTTTGTTTGAAATGA880.10654139980870976No Hit
ACCACAGGGGCTCTGGAATTTGGTGGGTAAGTTACCTTGAATGTGTATCTTT870.10533070208361078No Hit
GTGCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA870.10533070208361078No Hit
GAACATGGGGTTTTAAGGACTGACTGAGGCAGTTTGAGGATTAGTTTAGAAC860.1041200043585118No Hit
CCATTAGGGGTCTGGATTGAAATAGGCTGCTAAGTATAAAATACACACCAGA860.1041200043585118No Hit
AAGCTGGGGGGAAACTCCTGTAACCCCATTTGGGGTTTCTTTCTAAGCCAAT850.10290930663341283No Hit
CATAGAGGGATGATCACCTCAAGTTTCATTTTGAGTAATTTTCACCTTTGAA850.10290930663341283No Hit
ATATTAGGGCGATCTGCACGCGGTGAACTCATACACAAAAAATCCAACTATA850.10290930663341283No Hit
TACTCCGGGTTACAAACCATGATAAAAGGAGTGTATAGTGTTGAGAGTGCTT850.10290930663341283No Hit
GATACCGGGAAGTTAATTTATAATTTTAATATGACTCCAATAAAAATATCAT850.10290930663341283No Hit
CACATAAGGGGTAGTTTTTGCAAGGCATTAGAAAAAATTTCACAATTACAGA840.10169860890831385No Hit
CATGACGGGTCCCAGAGCGAGCCCAAACTGTCTGCCCAAATGAAAGCTGTGT840.10169860890831385No Hit
ACGCATGGGGTTAACGGATGCCTGGTGGCCCCTTCTGAGCCTAAGTACCTTT840.10169860890831385No Hit
CAAGGAGGGATAGGTTGCCTGTTCACTCTGATGGTAGTTTCTTTTGCTGTGC830.10048791118321487No Hit
TAAATCGGGGTGCTGCTGCTTGCAGCCAAAATGCCAGGTTCTGAAAAAAAGC830.10048791118321487No Hit
GTCGCTGGGTTGATAATGTGGGACATATGTGTGATTCAATATATACATATAC830.10048791118321487No Hit
CTTTATCCGGGAGCTTGAAGACAGACTATTTGAAAATATTCAGTCATAGGAC830.10048791118321487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)