FastQCFastQC Report
Sat 31 Dec 2022
SRR5847153.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847153.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82946
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC3200.3857931666385359No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT2750.3315410025799918RNA PCR Primer, Index 34 (100% over 21bp)
GCGCACGGGATATGTCAATATTTAGAACATTGCATAACTCACTGAACCAATA1600.19289658331926796No Hit
AATAGCGGGGACAAACCCATCATAAATTGAGGAGTGTATTTTATATGTATCA1440.17360692498734118No Hit
AGCCAGGATGGAAACGCGTGTCCCAAGTGTCCGGAGCAGGCGGCAGAGGCCT1200.14467243748945097No Hit
ATAACCGGGCACCAAACTGGAGGATGCCTATGTATTCCCTGGGGATGGCGCA1170.14105562655221468No Hit
GTAAGCGGGCTTCGCTGGCCATTTAGGAGCTCTGCTCAAAGCCAGACGTATC1160.13985002290646928No Hit
TTTAACGGGACGGGAAAGACTAAAAAATGACATCCAAAAATGAGCAAAAATT1110.13382200467774216No Hit
ACGGCAGGGTGCGGGGACGCGCTCGCGGCGGGACGCGCCGCGGTGGGGGATG1090.13141079738625128No Hit
ATCAAAGGGTGTCAATAGAACCTGAAAAAAGATTTTTTTAGTTGCCTCTAGT1090.13141079738625128No Hit
GTAAGCGGGCGCTCTTCCGTACTTGCCCAGGGAGCAAAATAAAATAAAACAA1030.12417717551177876No Hit
TCATGCGGGATTGAACTTTAGTTTCAAATCTTCACTCAACTGTACTTCCCCT1030.12417717551177876No Hit
AGACTAGGGACTTGATGCTTGTGTTAGAATTGTATTTCTCTTCTTCAGATGG1030.12417717551177876No Hit
GGTTTAGGGACATTAACTCCTTACTCTAAAAACCTTAAATCTATTTTATCAT1020.12297157186603332No Hit
GATATGGGGGACAGTGTTGAAGCAGGGTGAATAACTAGGGCATATATATTTT1020.12297157186603332No Hit
TACAACGGGTGTTTTAACAAGGTTTCTGGTGAAGACAAGCTATCAGTCTCTT1020.12297157186603332No Hit
TATTCAGGGACTCTTTATTTTCAAGCCTACAAATAACTTAACATGAGATGAA1000.12056036457454247No Hit
GTTCTAGGGAGTGAGCCAGGATTTGATTTCACTGCCTTATATTCTATTTTTA990.11935476092879704No Hit
ATTAAAGGGAAATGACCACTGGGTAAATAACGAAATTAAGGCAGAAATAAAT990.11935476092879704No Hit
CTAGCAGGGATTTGCTTGGATGTCCAGGTATAGGTGAATGCTTAGGAATATA980.11814915728305163No Hit
GTTGCAGGGTAAGTCAGTTGTCATGGTGTTTGTGGTGTGTATGTGTGTATGT960.11573794999156077No Hit
GCTTGTTGTCCTGAATTTTTTACCCCATTAGCAGTATCACTGAGTTGCAAAT950.11453234634581536No Hit
CAAGGAGGGATAGGTTGCCTGTTCACTCTGATGGTAGTTTCTTTTGCTGTGC930.11212113905432451No Hit
CCACTTGGGTAACACAATTCAGTGGTACTTGGAATCCTGTTTTAATTTGCAC920.11091553540857907No Hit
AGAAAAGGGAGTTACATGAGGCTTTTATGAAATTCTGTTTTGTTTGAAATGA920.11091553540857907No Hit
ATTGAAGGGCAGCCAAGCTTCTTGAAAGGCCTGTCTACACTTGCTGTCTTCC920.11091553540857907No Hit
ACTATAGGGATGAGGAAAGGTCTACTAGATGTGCTTCCAAAAAATTCATTAG910.10970993176283364No Hit
TTGGATGGGTGGTTTTAGATGATAACCACAGGTCTACAAGAGCGTTTTTAGT910.10970993176283364No Hit
ATTCTAGGGCTCTTCCGATCTCCGCGTGGGGATCACTGGTTTTTCCCTCCTG910.10970993176283364No Hit
AAGAAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC910.10970993176283364No Hit
CATGTGGCGATTAGTGTTATCCTCATCCCTGTTTTACAGATGAGAAAACTGA900.10850432811708823No Hit
GTGCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA900.10850432811708823No Hit
GTGAGAGGGGTGTAAGGAACTCTTAAAATTCCACAAGAAAACAAACAACCCA900.10850432811708823No Hit
AGGCCCAGGTCGGGCTTCCCACCTCAGCCAAGCCTGCCCCTTTGCAGGGCCC900.10850432811708823No Hit
GTCAGTTGGGGTGTTTTGTTTTGTTTTGTTTTACCTGTTTGGGGTATTTTTT900.10850432811708823No Hit
TATAGCGGGAATCACTAAATTAATCTCTCTCTCTTCTCTTTGAACAGCTTAG890.1072987244713428No Hit
ATGATGGGGTATTGGCAGTTCAGATTGTGTTTTCCCAACTTAGGCTCTTTAT890.1072987244713428No Hit
GAGAATGGGAGACCAAATTTACCCTGGTGGTCCCATAAGATTCTAATACTGT890.1072987244713428No Hit
ACGCGCGGGGGTTTTGAGTCATCCAGTCCTGGGCACTGAGGTCTGTTAGATG880.10609312082559738No Hit
ATGCTTGGGATCTCAGCTCACCGCAACCTCCGCCTCCCAGGTTCAAGCGATT880.10609312082559738No Hit
CAAATAGGGAGAAGAGCAGCGTGAACAGGAAGCTAAGCATGAACGCATTGAA880.10609312082559738No Hit
TGTGGTGGGCAGAGGATATGTTGCAAAGAGATTGATTAGATTTTCTTTTCTC880.10609312082559738No Hit
CACATAAGGGGTAGTTTTTGCAAGGCATTAGAAAAAATTTCACAATTACAGA870.10488751717985195No Hit
GTAGTTGGGGAGGTCCAGCGGGTGCTGTGATCTTCAAGTATGGGGTCTGCTG870.10488751717985195No Hit
TATCCAGGGTCCATTCCTGTGCATTGCCTTTTTTCTGGCTGTCACGCATTCT870.10488751717985195No Hit
TTGGGTGGGTTGTCATCTTCAGAGTATATAACTGCTGGGATTCAGTGCGTCA870.10488751717985195No Hit
TGATATGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG870.10488751717985195No Hit
ATTTTAGGGAGATGGGATCTAATTAAACTGGAGAGTCTGCATGGAGAAGGAC870.10488751717985195No Hit
GATAAGGGGGATCGCCCAGTGGCGCCTGTCCGTGTTTTGGCGCCCCCTAGCA870.10488751717985195No Hit
CTTTGTGGGAGGGCAGCCTGGCGCGCGGCGCCCGCCAGGCGCCCAGTCCTCT870.10488751717985195No Hit
TTATGGAGGGCCCCAAGAGCATGCTGCCTTACAAGACAGGTGAAAAATGTGT870.10488751717985195No Hit
AAGTGAGGGATGTGACCTCATCTAGCTATGAATTCTGGGAAGTCAATGTGAA860.10368191353410652No Hit
CCTTCTGGACTCCACACTCCCCAGATCCCAGTATGACTACATCTTGCCTCAA860.10368191353410652No Hit
ACAGATGGGGATCTTCGTGGGGAAAATCCAGGTCGCGATCGAAGAGTCCCCC850.10247630988836111No Hit
AAACGTGGAATTGCTTGATCATATGATAATTCTATGTTTAATTTTTCAGGAA850.10247630988836111No Hit
TTGAGGGGGATAAATGATGATGAGATTTGAATTGTCTGTGTTCTCACCTGCC850.10247630988836111No Hit
GATTAAGGGTCACTATTAATGAAATGTGTAAATTCTCCAGAGTACCTGATTT850.10247630988836111No Hit
AGTAACGGGATATCAAATGCAAACACAGCACTTTCTCAGACTCACTTATAAG850.10247630988836111No Hit
GATAACGGGACCTATTTCCAAGCCAGTATAAGAAACTATTAACTTTGGGTCC850.10247630988836111No Hit
CTTTATCCGGGAGCTTGAAGACAGACTATTTGAAAATATTCAGTCATAGGAC850.10247630988836111No Hit
CGAACAGGGTATCCACATTAAAATTTGAAATCTAAGTGAATTACTTCAAGCT840.10127070624261568No Hit
GGAATAGGGAAGTGCCAGCAGCTGCACAGATACTTCTCTATTCAGCCAATTC840.10127070624261568No Hit
ATGAGGGGATATTCTTGTATATTTGAAAAATAACTTATTATTTGAATTGTTG840.10127070624261568No Hit
GAATGGGGGCTGGCAAGATAAACACTTTCTGGAACCCGAGACTTGGCCATAA840.10127070624261568No Hit
TAAATCGGGGTGCTGCTGCTTGCAGCCAAAATGCCAGGTTCTGAAAAAAAGC840.10127070624261568No Hit
GCGATAGGGACACATCTATCACCTCCCCTGCTATATATTAGACCCTGAGATC840.10127070624261568No Hit
ATTTATGGGAGGCTAGGGGGATGACATGGTTTCCTTTTAGGTTTTAGAAGAA840.10127070624261568No Hit
GCACCTTGGGAGGGAAGTCACTTTACCTTGCGGTGGTGGGATCCTGATGTCT840.10127070624261568No Hit
AGCGGCGGGGGTTGTCAAATCATATAGTTGTCACTGCTAATCATGGAGAAGT840.10127070624261568No Hit
CTGAGGGGAGTCACTGAAGAGGGAAAAGGAGTGAGAAGACAAAGCCGTCAAA830.10006510259687026No Hit
AGATAGGGGATTTCTAATGTGTGTGTCTGTTTTTAAATTGATATACAGAGAA830.10006510259687026No Hit
GGCTAGGGGACTCTTCCCCTGATAGAAAGTTGTCTGGGACTTTCATGAGTGA830.10006510259687026No Hit
CTGCTCACTGCAACCTCTGCCTCCCGGGTTCAAGCAATTCTCTGACTCAGCC830.10006510259687026No Hit
TTTATTGGGACTATCGGACAGAATTGAATTTGGGAGAGAAAAATATTAAATA830.10006510259687026No Hit
ACCACAGGGGCTCTGGAATTTGGTGGGTAAGTTACCTTGAATGTGTATCTTT830.10006510259687026No Hit
CTGTAATAGGGGCAGGAACTAGAAACACATCTTCAGCCTCCTCCTGGGCCAG830.10006510259687026No Hit
AACTCTGGGACTCCTAAGTGGAGGACTTTCATGAGCCTATCTCATGACTGGC830.10006510259687026No Hit
ACTTAAGGGGCATGCATTGAGCTGCCTCCAGCCTGGTGGCCCAGCTATTACC830.10006510259687026No Hit
CATGACGGGTCCCAGAGCGAGCCCAAACTGTCTGCCCAAATGAAAGCTGTGT830.10006510259687026No Hit
GGCTAAGGGAGGAGCCGACTCTTTGATATGCCACCAGCGAACTCTCAGAAAT830.10006510259687026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)