FastQCFastQC Report
Sat 31 Dec 2022
SRR5847152.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847152.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81792
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC3120.3814553990610329No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT3000.36678403755868544RNA PCR Primer, Index 34 (100% over 21bp)
AATAGCGGGGACAAACCCATCATAAATTGAGGAGTGTATTTTATATGTATCA1270.15527190923317685No Hit
GCGCACGGGATATGTCAATATTTAGAACATTGCATAACTCACTGAACCAATA1250.15282668231611893No Hit
CAGGCTGGGCTGCCCGCACCTGCTGTTCATGGTTCTAATAGGTATTTCAGGA1220.14915884194053208No Hit
ATCAAAGGGTGTCAATAGAACCTGAAAAAAGATTTTTTTAGTTGCCTCTAGT1190.1454910015649452No Hit
CCAATTGGGTCATCCAGTCATCGCTAATATTACACGCACCTTCTCACTTAAT1130.13815532081377152No Hit
TCGATCGGGGTACACCCAAGCCAATGGACTGGATTCCTATTTTAACTAGAAA1120.13693270735524257No Hit
AGACTAGGGACTTGATGCTTGTGTTAGAATTGTATTTCTCTTCTTCAGATGG1080.13204225352112675No Hit
AGTAACGGGATATCAAATGCAAACACAGCACTTTCTCAGACTCACTTATAAG1050.1283744131455399No Hit
TTTAACGGGACGGGAAAGACTAAAAAATGACATCCAAAAATGAGCAAAAATT1030.125929186228482No Hit
TCATGCGGGATTGAACTTTAGTTTCAAATCTTCACTCAACTGTACTTCCCCT1030.125929186228482No Hit
GGTTTAGGGACATTAACTCCTTACTCTAAAAACCTTAAATCTATTTTATCAT1020.12470657276995305No Hit
TACAACGGGTGTTTTAACAAGGTTTCTGGTGAAGACAAGCTATCAGTCTCTT1010.1234839593114241No Hit
ATTTTAGGGAGATGGGATCTAATTAAACTGGAGAGTCTGCATGGAGAAGGAC1000.12226134585289515No Hit
GGCTAGGGGACTCTTCCCCTGATAGAAAGTTGTCTGGGACTTTCATGAGTGA990.12103873239436619No Hit
TTGAGTGGGGATTCAGATCTAACCCTTCTATGAGTTCATAAAATGGTAGAAT970.11859350547730829No Hit
GTGCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA960.11737089201877934No Hit
TGTGGTGGGCAGAGGATATGTTGCAAAGAGATTGATTAGATTTTCTTTTCTC940.11492566510172145No Hit
TCTATGGGGCTACGGCCGGGGCATACAATAAAAAGATTACTAGGTATGCAAA920.11248043818466354No Hit
CTGCTCACTGCAACCTCTGCCTCCCGGGTTCAAGCAATTCTCTGACTCAGCC920.11248043818466354No Hit
CATGACGGGTCCCAGAGCGAGCCCAAACTGTCTGCCCAAATGAAAGCTGTGT920.11248043818466354No Hit
TTGGGTGGGTTGTCATCTTCAGAGTATATAACTGCTGGGATTCAGTGCGTCA910.11125782472613459No Hit
AGCCAGGATGGAAACGCGTGTCCCAAGTGTCCGGAGCAGGCGGCAGAGGCCT910.11125782472613459No Hit
TTCACGGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG910.11125782472613459No Hit
ATTCTTGGGACCTAGTGGGACCTTCCAGAGGCTAAACAGACCATTGTGGCTC910.11125782472613459No Hit
AAACGTGGGGGCCAGTTGTGGGGTTGGTAAGCACAGTTATGAAAATTTCTAT900.11003521126760564No Hit
GTAAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA900.11003521126760564No Hit
AAAAGCGGGGTGTCAAGAGATTGACTCGGCATTGCCTTCATTCCTTAAAATT890.10881259780907669No Hit
CTGCTGGGGTACAATCCATCTGTAATTAAGTATTTGGAGAACAGAATATGGC890.10881259780907669No Hit
GTAAGCGGGCTTCGCTGGCCATTTAGGAGCTCTGCTCAAAGCCAGACGTATC890.10881259780907669No Hit
AATACGGGGTCTGCAAATGGGGAGTTACAGGGAACTTGAGGAGAAAAGCAAA880.10758998435054773No Hit
TGACATGGGTGGCCTTTGCCGTAAGCACATTCTCTGTCTTACACTTGAACTT880.10758998435054773No Hit
CACTAAGGGATGTGGTAGTTTGGTATTTCAGATAGCTAACTTTTAACTTTTT880.10758998435054773No Hit
AGGCCCAGGTCGGGCTTCCCACCTCAGCCAAGCCTGCCCCTTTGCAGGGCCC880.10758998435054773No Hit
AAGAAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC870.10636737089201878No Hit
CATGTGGCGATTAGTGTTATCCTCATCCCTGTTTTACAGATGAGAAAACTGA870.10636737089201878No Hit
TCGTGCGGGGCTATTACTTAATTATTTTCCTCTGTTTTAGACTATAATCTTT870.10636737089201878No Hit
AGGTCCGGGCTGGCGGTGGGTAAACTCAAGCTGGGAACTGGGGAGTGGGATC870.10636737089201878No Hit
GAGAATGGGAGACCAAATTTACCCTGGTGGTCCCATAAGATTCTAATACTGT870.10636737089201878No Hit
TTATGGAGGGCCCCAAGAGCATGCTGCCTTACAAGACAGGTGAAAAATGTGT870.10636737089201878No Hit
GTTCTAGGGAGTGAGCCAGGATTTGATTTCACTGCCTTATATTCTATTTTTA870.10636737089201878No Hit
ATTAAAGGGAAATGACCACTGGGTAAATAACGAAATTAAGGCAGAAATAAAT860.10514475743348983No Hit
ACTTAAGGGGCATGCATTGAGCTGCCTCCAGCCTGGTGGCCCAGCTATTACC860.10514475743348983No Hit
CCTTCTGGACTCCACACTCCCCAGATCCCAGTATGACTACATCTTGCCTCAA860.10514475743348983No Hit
ACGCATGGGGTTAACGGATGCCTGGTGGCCCCTTCTGAGCCTAAGTACCTTT860.10514475743348983No Hit
AAACGTGGAATTGCTTGATCATATGATAATTCTATGTTTAATTTTTCAGGAA850.10392214397496088No Hit
GCAAGTGGGTGGTCCAGTATTTCACAGCCATCGGCTACCCCTGTCCTCGCTA850.10392214397496088No Hit
TTACAAGGGTAGTGGAGTGAAAATGCCAAATCCTATGGATTTCTCCTTAACC850.10392214397496088No Hit
CTTTGTGGGAGGGCAGCCTGGCGCGCGGCGCCCGCCAGGCGCCCAGTCCTCT850.10392214397496088No Hit
ACAATAGGGTGCAGTCTGGAGAGAACTTCTGTGCTTTAGAGCACCAGAGCTG840.10269953051643194No Hit
ATCTGGGGGGAGGACCTCCCTCAGATACCAGAATCCTACATAAAATGATGTA840.10269953051643194No Hit
ATAACCGGGCACCAAACTGGAGGATGCCTATGTATTCCCTGGGGATGGCGCA840.10269953051643194No Hit
GTAACCGGGATGTGATAATTGAAAATATTAACTATGTGCTTAGATTATTATT840.10269953051643194No Hit
TATCCAGGGTCCATTCCTGTGCATTGCCTTTTTTCTGGCTGTCACGCATTCT830.10147691705790296No Hit
CACAATGGGTAAGGGCCGAAGATAACGCGGATACCTTGGCGCTAGTATTTGA830.10147691705790296No Hit
CTTCGGGGGGAAGGGCTGCGGTAGTGATTCCATTGATTCCTGCTTTGATGGA830.10147691705790296No Hit
GAATGGGGGCTGGCAAGATAAACACTTTCTGGAACCCGAGACTTGGCCATAA830.10147691705790296No Hit
CTTACAGGGATTAAATTATATCTGAGGCACTGATAATATGTTTGAGGTTAAA830.10147691705790296No Hit
CAACCAGGGGAGTCATCTAAGCAGAGTGCTGCAAAAGAAAAGGATTTGTTGC830.10147691705790296No Hit
ACATCTGGGAGGTAAGGCACCAACTGTGTCTGCTACTGTTTTTATTTTTTAG820.10025430359937401No Hit
TGATATGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG820.10025430359937401No Hit
ACATAGGGGAAATGTTATTTAGCTTTGAAAAGAAATGAAATTCTGACACATG820.10025430359937401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)