FastQCFastQC Report
Sat 31 Dec 2022
SRR5847148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123492
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT6580.5328280374437211RNA PCR Primer, Index 34 (100% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC6300.5101545039354777No Hit
AATATCGGGAGTTTATCACTACTAGAAGCAAGAGAAGCAGACGGTGATGTGG1430.11579697470281475No Hit
GATAGTGGGCTTTATTCATATTTGAAACAAACAATTTTCTTATTTGTTCACC1390.1125578984873514No Hit
GACTGTGGGATGTCCAGAAAGCCAACCCAGAGCCTGCAGAAGGCTAATAAAA1380.11174812943348558No Hit
TGCTTAGGGGATAATGATGGTATTAACAGATGAGGAACATAGCAAGACAAAC1380.11174812943348558No Hit
GTATGTGGGGGTAGCCATTTCCTGACATCAAGATGTTGAATGTAATCTGGTC1370.11093836037961974No Hit
CCAATAGGGCATTTATATGAAATGTTCAGAAGAGGCAAATCTACAGAGACAA1320.10688951511029054No Hit
AACGCTGGGGGGAATAAAGGGATAAGGAGATGGTGGGCATATAGAAGCTGAG1320.10688951511029054No Hit
ACTTTGGGGTGTCTACAAACAAAGTGTTACATCTTCTGCATAAAATTACCTA1300.10526997700255887No Hit
CTGAGACGGGGACTTCAAATTCCTCCCTGTACGAAAGGACAAGAGAAATAAG1300.10526997700255887No Hit
TAGTTTGGGGGGAGAACTCTTTCTTCTACTGTGGATTTTTCAATAGCCCCTT1280.1036504388948272No Hit
ATCGCAGGGTAGTAGCAAAAACTTGGGAATGACCAAAATAACCATTAAAAAG1270.10284066984096135No Hit
CATACAGGGATGTTATTTTCAAGGATAAAATACCAACTTTTGAAATAAAGAT1270.10284066984096135No Hit
TCGTTAGGGAAAGTCAAATGTCTCATTTGATAGAGAGCAGCAGAGTTCCTAC1260.10203090078709552No Hit
TAATGAGGGGCGTCTGGGATGTGAGGAGCACCTCTGCCCTGCCGCCCTGTCT1260.10203090078709552No Hit
TCGACAGGGGTAAGGAGTGCGGGAAAGCCTTCAGTTCTTCCAGTTCCTTTCG1250.10122113173322968No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)