FastQCFastQC Report
Sat 31 Dec 2022
SRR5847147.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847147.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122861
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT7080.5762609778530209RNA PCR Primer, Index 34 (100% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC6220.5062631754584449No Hit
TAAAGGGATTGAAGGACTGGAGTTCATGGGCCATGGCAAAGAAAAGCCTGAA1470.11964740641863568No Hit
CTGAGACGGGGACTTCAAATTCCTCCCTGTACGAAAGGACAAGAGAAATAAG1390.11313598294007048No Hit
GGCAACGGGGATTTGCATTTCTCTGATGATTACTGATGTGGAGCATTTTTTC1370.11150812707042919No Hit
GGAAAAGGGATTACCAGGTCCCACGTACCTCGGAGATTTATGTCCACCGAAG1360.11069419913560853No Hit
TCGACAGGGGTAAGGAGTGCGGGAAAGCCTTCAGTTCTTCCAGTTCCTTTCG1340.10906634326596723No Hit
CGTTGCGGGTATTGAGATTGGTCAATGCATTTCATAATTCTTTTATGTCTCT1330.10825241533114659No Hit
ACACCAGGGAGCCAATAAAAATGAGAGAACAAAAGTAACTCAGTTAAGCTTA1310.10662455946150527No Hit
AACGCTGGGGGGAATAAAGGGATAAGGAGATGGTGGGCATATAGAAGCTGAG1310.10662455946150527No Hit
GTATGTGGGGGTAGCCATTTCCTGACATCAAGATGTTGAATGTAATCTGGTC1300.10581063152668463No Hit
ATAGCCGGGAAGTCACTTTTGACATTGATGCCAATGGTATCCTCAATGTCTC1300.10581063152668463No Hit
TGCTATGGGATGTTGCAGACAGTTGCTGCTTTGTTCCAATGAAACGATGTGT1290.10499670359186397No Hit
AGAAGGGTAGCAGTTTAATTTTCATCAAACATCGGAGTAGTTTAGTTGTTTG1280.10418277565704331No Hit
GTTTATGGGCAGCTTGACCACAGCGTTTGGTTCTTTCTTTAAGTGTTGTGTT1280.10418277565704331No Hit
TACACAGGGATCATGGAAATGTACATTTTTGGTTTAAAATATATAAGAAAAA1280.10418277565704331No Hit
GCATAAGGGGGTAAGTACTATTATTAACCTAAGTTATAGCTGAAGAAACTCC1280.10418277565704331No Hit
TATCCTGGGGATCTTACTGCGGGGCTTGAGAACTGGGGAATCTCCTACTTTT1270.10336884772222268No Hit
CATCTGAGGGAGGAAAACATGACTTGCTAATATACATGAAAAATGGTCAACT1240.10092706391776071No Hit
ATGAGAGGGAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGATTAACGTCC1240.10092706391776071No Hit
GAGCAGGGAATGTTATTGCCTTTGTAAATTTAACTTTTCAATTATAAATTTA1240.10092706391776071No Hit
AATATCGGGAGTTTATCACTACTAGAAGCAAGAGAAGCAGACGGTGATGTGG1230.10011313598294008No Hit
TAATGAGGGGCGTCTGGGATGTGAGGAGCACCTCTGCCCTGCCGCCCTGTCT1230.10011313598294008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)