FastQCFastQC Report
Sat 31 Dec 2022
SRR5847146.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847146.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119847
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT6100.5089822857476616RNA PCR Primer, Index 34 (100% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC5910.4931287391424065No Hit
CTGAGACGGGGACTTCAAATTCCTCCCTGTACGAAAGGACAAGAGAAATAAG1420.11848440094453762No Hit
TGCTTAGGGGATAATGATGGTATTAACAGATGAGGAACATAGCAAGACAAAC1410.11765000375478735No Hit
AATATCGGGAGTTTATCACTACTAGAAGCAAGAGAAGCAGACGGTGATGTGG1360.11347801780603603No Hit
ACACCAGGGAGCCAATAAAAATGAGAGAACAAAAGTAACTCAGTTAAGCTTA1320.11014042904703497No Hit
GAGCAGGGAATGTTATTGCCTTTGTAAATTTAACTTTTCAATTATAAATTTA1300.10847163466753444No Hit
TGCTATGGGATGTTGCAGACAGTTGCTGCTTTGTTCCAATGAAACGATGTGT1280.10680284028803391No Hit
TAAAGGGATTGAAGGACTGGAGTTCATGGGCCATGGCAAAGAAAAGCCTGAA1280.10680284028803391No Hit
TAGTTTGGGGGGAGAACTCTTTCTTCTACTGTGGATTTTTCAATAGCCCCTT1270.10596844309828364No Hit
GCAAGCGGGGGGAAGTCCAAAGTCAAGAGCAGAAACAAAAGACACTAGAGGA1270.10596844309828364No Hit
TCGCTAGGGACTATAAAAAGGAGAAAATGGGTATTAATTTCTACCCTCCATG1260.10513404590853338No Hit
TATGTAGGGCACCAGGGCTGACTTTGACCGAGGGCCATGGACGCGACAGGCC1250.10429964871878311No Hit
GATAGTGGGCTTTATTCATATTTGAAACAAACAATTTTCTTATTTGTTCACC1250.10429964871878311No Hit
TTAGAGTGGGGAAATACACACCTACATGGAAATGTTTCAACGTGCGCAAGCG1240.10346525152903285No Hit
GTGGTCGGGAAAGAGGGAGGCTTGGGCCCCCTGAACATCCCCCTGCTTGCTG1230.10263085433928258No Hit
AACGCTGGGGGGAATAAAGGGATAAGGAGATGGTGGGCATATAGAAGCTGAG1220.10179645714953232No Hit
TGCAGTCGGGGGGACTTACGTCAGTGAGCTTAACATCTTTCTCATTAATGAG1220.10179645714953232No Hit
TTAGCTGGGATAGTGTGATGAGCAAGGCTGTTGCATCAGGTTTTTAATTTAA1210.10096205995978205No Hit
ACTAGCGGGAAGGCAGCACAGACAAGTGTGATCGGTAGAAGTCATGGTGAGT1200.1001276627700318No Hit
TAATGAGGGGCGTCTGGGATGTGAGGAGCACCTCTGCCCTGCCGCCCTGTCT1200.1001276627700318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)