FastQCFastQC Report
Sat 31 Dec 2022
SRR5847142.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847142.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159946
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT7040.4401485501356708RNA PCR Primer, Index 34 (100% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC4290.2682155227389244No Hit
CATCAAGGGCCACTGGTTTGGTGGGGAACTCACAGTAATTCCAAATGTACAA1970.12316656871694197No Hit
AACGCTGGGAAGGGTTCTGAACTTGCAGAATACCAGATAATTGACATATCAG1830.11441361459492579No Hit
GTTTGCGGGTAGAAGATAATAGGATATTTGTCAACTCTTTGAAGGATAGATG1830.11441361459492579No Hit
ACACTTGGGTAATGATTTGAGTATTAAAATTTCAGTAATGTATTATGGTCAT1770.11066234854263314No Hit
AGCATTGGGGGGCGGGGTCTTCAGGGTACCGGGCTGGTTACAGCAGCTCTAC1760.1100371375339177No Hit
ATTACAGGGATTCTGAACAGGGACGGACTCAAAAGTGTATGTGTGAATATAT1740.1087867155164868No Hit
ATGATGGGGGCCACCAGTTGAAGAGAACAGAATCCACACCTGCATTGAATAC1730.10816150450777137No Hit
TTCAGTGGGCATTCTGAGAAATGCATCCTTAGGTAATTTCATCATTAGGGGA1720.10753629349905593No Hit
ATGTTAGGGTGACTGATCCTAAGCTGCGGAGAAAAGGTGTTCAGCCTCCAGG1710.1069110824903405No Hit
ATCCAAGGGTAGTTTTAAAAAAATATATCCTAAATACAAGTCTCTTATCAGA1700.10628587148162505No Hit
GTAGACGGGGATGGGACCCCAGCGAGAGATCTGCAGCTAGGCTGGCTGCACT1680.10503544946419416No Hit
GTTAGTGGGAAGACACAACTCTGAATTCTAGCAGTGAGTACTGTGTGGAAAA1650.10315981643804784No Hit
CAACAGCGGGATGACTCAGCAGGGATGACTCAGCACCACCCCTCAACAGGGA1620.10128418341190151No Hit
TCCGTTGGGAGCCCCAGTAGCTTCCCATTTGCTCTTGGGATGAAGTCCTAGT1620.10128418341190151No Hit
CTTTCCGGGAACCACAGGAAAGAAAAGATACCCAATACGGTAATAAAAATGA1600.10003376139447062No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)