FastQCFastQC Report
Sat 31 Dec 2022
SRR5847141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160375
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT6510.40592361652377246RNA PCR Primer, Index 34 (100% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC4370.2724863600935308No Hit
GTTTGCGGGTAGAAGATAATAGGATATTTGTCAACTCTTTGAAGGATAGATG1970.12283710054559625No Hit
TGCCAAGGGAACTTCTGGAACTGATAAGTGAATTAAACAAGATTACTGGATA1830.11410756040530008No Hit
AGCATTGGGGGGCGGGGTCTTCAGGGTACCGGGCTGGTTACAGCAGCTCTAC1810.11286048324240062No Hit
GCTCAAGGGTATTGAGATAATGCCAAATTGTTTCCTTCAGTGGTTTCACCAT1780.11098986749805144No Hit
GCTGTGTGTGCCTCTGTAGCTCCTCCTGACAAACGTCTGGGAAAACAGCCTC1770.1103663289166017No Hit
AACAGAAGGGTGACTAAAACTACTTCTTTTCAACATGTGACCCCTTCCCCAC1720.10724863600935308No Hit
CAACAGCGGGATGACTCAGCAGGGATGACTCAGCACCACCCCTCAACAGGGA1710.10662509742790334No Hit
AGTTCAGGGTCCTCACGGCTTGGAAGACTGGTCATTTTTTGCAGTTTATGAT1700.10600155884645363No Hit
CGTTTGGGATGAATGGCTGCTAGGACCTTTAGTGCTCCCTGCACCAACCTCC1700.10600155884645363No Hit
TCATATGGGATACATAGTCCAGCCCCTCTTCTGCCTAGAGATTTCTAACTTC1700.10600155884645363No Hit
GTAGACGGGGATGGGACCCCAGCGAGAGATCTGCAGCTAGGCTGGCTGCACT1680.10475448168355418No Hit
AACGCTGGGAAGGGTTCTGAACTTGCAGAATACCAGATAATTGACATATCAG1670.10413094310210444No Hit
TAAAGTGGGTAAAATATGGATAATCCAAATAACTTCTTTCTAATGTGATTTA1670.10413094310210444No Hit
GCAATGTGCGTTTTCTTAATGCCCATCTGCAGGTGAGGAAATGAACTCTGGG1650.10288386593920498No Hit
CTAATAGGGATGCACAGAATTTCCATCTATCAAGGACAGAGGACCCAGAGAC1640.10226032735775525No Hit
AAACCGGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAG1630.10163678877630554No Hit
AACCAAGGGATGCAAGCCATTTGCACTCTGGAACTGCATGCCGTGAAAACTC1610.10038971161340608No Hit
CCATCTCGGGAAGAAATGAATGGAAGGAAATATGATCCTCCTGAGACTAACT1610.10038971161340608No Hit
GTTAGTGGGAAGACACAACTCTGAATTCTAGCAGTGAGTACTGTGTGGAAAA1610.10038971161340608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)