FastQCFastQC Report
Sat 31 Dec 2022
SRR5847140.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847140.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158102
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT6520.41239200010120053RNA PCR Primer, Index 34 (100% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC4130.26122376693526966No Hit
AGCATTGGGGGGCGGGGTCTTCAGGGTACCGGGCTGGTTACAGCAGCTCTAC1830.11574806137809768No Hit
CAACAGCGGGATGACTCAGCAGGGATGACTCAGCACCACCCCTCAACAGGGA1830.11574806137809768No Hit
GTAGACGGGGATGGGACCCCAGCGAGAGATCTGCAGCTAGGCTGGCTGCACT1830.11574806137809768No Hit
AACGCTGGGAAGGGTTCTGAACTTGCAGAATACCAGATAATTGACATATCAG1800.11385055217517803No Hit
CCATCTCGGGAAGAAATGAATGGAAGGAAATATGATCCTCCTGAGACTAACT1650.10436300616057989No Hit
ATGATGGGGGCCACCAGTTGAAGAGAACAGAATCCACACCTGCATTGAATAC1640.10373050309293999No Hit
TGCTTAGGGGGAAGACAGTGTGGCGATTCCTCAAGGATCTACAACCAGAACT1620.10246549695766025No Hit
CTTTCCGGGAACCACAGGAAAGAAAAGATACCCAATACGGTAATAAAAATGA1610.10183299389002036No Hit
GCAATGTGCGTTTTCTTAATGCCCATCTGCAGGTGAGGAAATGAACTCTGGG1600.10120049082238049No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)