FastQCFastQC Report
Sat 31 Dec 2022
SRR5847118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196646
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT7960.40478829978743525RNA PCR Primer, Index 34 (100% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC7890.40122860368377694No Hit
TCATACGGGAGACTCTTTCCATACTGTAGTATTGGGAAGGAAGTCCCTATGT2660.13526845193901732No Hit
GTACCTGGGCTTCATTCCACTTTTACCATGTTTGTTTGCTTCCTTTTATGGA2530.12865758774650896No Hit
ACTAAGGGGCAGTCTTGATATTTTGCAACATTGCTGATGTGTGGATAATTTA2470.12560641965765892No Hit
ATAGTGGGGAATTAGACATTTGTGGGCACAGGGTTCTTTTGTTTTTGGGTTT2430.1235723075984256No Hit
CGCCACGGGCATGTAGGCTTGAAAGGCAAGACTTTTTGGAAGAGCATGGTTT2420.12306377958361728No Hit
TTCGCCGGGCAAACCTAAAAGTAGGTATTTGTGTAGACATAAGTTTACTATT2350.11950408347995892No Hit
CGCATAGGGGTGGCAGAAGGATGAATTGCGGGAGACTCTCAAATCCCTAAAG2350.11950408347995892No Hit
TTTGATGGGATCTCTAGCTCGACCCCAAACTGTATATTGATTTAATTAATAT2300.11696144340591723No Hit
GGTGCGGGAGAATTTCCCAGGGCACCTGCCGGCAGCTATCTTGATAGGAAAG2300.11696144340591723No Hit
TTTACAGGGTAACTAGCAATTCTACCCTAAATTGAGGTCCACTCTGAGAAAA2250.11441880333187555No Hit
GTAACAGGGGTCAAGTGATTATATTGCTTGTTTTCAAGAAACCTTCAGATTT2230.11340174730225887No Hit
CAATGCGGGATCTAAAAACACAAAACGTTAGGCCTTTTTCTTCTTGGGGACG2220.11289321928745055No Hit
ATGCTGGGGACAGCAAACCCCCATGACTTGAGTTTACCTATATAACAAGTCT2210.11238469127264222No Hit
CTAAAAGGGACTTGAACACAGGCACTGTTGTCCACCCACAGATAAAGCTTGG2210.11238469127264222No Hit
TGCGTCTGGGGAAGGGATTTTTTTAATGTCACGTGGCTCTCACAGGTGCGGT2210.11238469127264222No Hit
GCCTTAGGGGAGCTCGAGGAAGTCACCCCATTAACTGACACACTGCTGTTCT2210.11238469127264222No Hit
TAACTAGGGGCAGATTTATGCCTTATTTTTTAGCATTTTTTAAGGTTGGGTC2200.11187616325783387No Hit
CCCACATCGGGTGGATTTCTGAAGCTCTCCAAGCCCTGCTGTTATATTTTTC2200.11187616325783387No Hit
TAAACAGGGGTGTTTTGCTTGTGTTTTTATAAGATATGGGATTTCTATTTGT2180.1108591072282172No Hit
TTAACTGGGCTCCATGGGTTGATTTTATGTATGCCTAGTATGTCCGAATGTG2160.10984205119860053No Hit
TATTGTGGGCATCGAGGGATGGCATTACCTCCGTGTTTCTCCCTTTCGAGCC2160.10984205119860053No Hit
ACTGCAGGCGCGTGCCACCATGCCCGGCTGATTTTTGTACTTTTAGTAGAAA2140.10882499516898386No Hit
GCGGCCGGGGAAATGTGAAGAAGCTTAAAGACCCTACCTTGCGGTTCCGGCT2130.10831646715417553No Hit
GAAAATGGGGGGCAGATCCCCAAAATCAAAATCATTGCCTTCTCGCAGATTG2130.10831646715417553No Hit
GTACACGGGGTAAAGGCCAAGCTGATTGTCTCTGGGTTCCGACACATCTCCC2130.10831646715417553No Hit
GGCTATGGGCAGGCCTTAATAGAAACCATAAGGCATGACTCATCTTCAGGCA2120.10780793913936718No Hit
TACCATGGGCACAAACTCTACTTCATTTTTCCTACCTCCTGTGCAGTGAAGA2120.10780793913936718No Hit
GCTTATGGGGTATGTGAAAATGGGACTGTGGATTTGACAGCTTTTTGAAGTG2100.1067908831097505No Hit
TAGATATGGGATTTTTAAAATTTCTTATTTATTCATAGACTTCCCTCTTACG2090.10628235509494217No Hit
CTAGCAGGGTTAATGGTTCCAATTTTATTTGCATCTACATAATAATTTTAAG2080.10577382708013384No Hit
CTGCAGGGGAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGGC2080.10577382708013384No Hit
AAGACAGGGGATGTGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGG2070.1052652990653255No Hit
GCCATGGGGAACCATCCCTGCATTCCACAGGGGACTCGGGCAAGGGTGCCGA2050.10424824303570884No Hit
GTCTTTGGGATTCTAACAATACACTATGTAGCAATGTGAAGTTTTATCCATA2050.10424824303570884No Hit
CATTGAGGGGATGAATGCATGGATGGGTGGATGAATAGATGGATGGATGGAC2050.10424824303570884No Hit
TAAGGTGGGGAGTTTAGCTTTGAGTTTGGGCCAATCCACAGATAGCTTAGGT2050.10424824303570884No Hit
GGGTCTGGGTCCCCCCAACCCCAAATTTGTCTAAGCACTGGCCAGTCTGTTG2030.10323118700609217No Hit
AAAAGAGGGGAGGCCCGTTTGGCGCCTTCACTCGGGAAGACGTCGGCCAGGA2020.10272265899128383No Hit
TCTGTAGGGATGTATCCAAACACAGAGACCCATGGGAACAAATGAGACACAT2010.1022141309764755No Hit
TCTGACGGGATTCTTGTCAGCTAAGGTCACATTGTGCCTTTTTGACCTTTTC2000.10170560296166717No Hit
CACATGGGAACAGCTGTAAGTGTTCACTTAAGAAACTGAAAGCTGTATGTCA2000.10170560296166717No Hit
GATGGTGGGGTTAATATGTATGTTAGAAACCCATCTATACTTGAGGTACAGT2000.10170560296166717No Hit
TTCAGCAGGGCGATCTAGATTTGGGACAAAGGGTCTGCGTAAATACTGAGAG1990.10119707494685881No Hit
GAGTTTGGGAGGTACTGATGATGTCAGATAAATGAATTTGCTCTTGCACAGT1990.10119707494685881No Hit
CGATAAGGGAACTGTCAAGCCTGTGGATGATTTTTTTGAACTTGGTAGTTCA1990.10119707494685881No Hit
TCACCTTGGGCGTACGGAATTAAGAAGGGTAAGGGGTGAAAATGAATGAGAA1980.10068854693205048No Hit
GTACTAGGGATATTGTCCTTGGACTTGGCTGTTGTATTATACTTTAAACGGA1980.10068854693205048No Hit
ATTGCCGGGTGCAATGCAAAATGTGTTTTCATAGGGAAAAAAGGGGGAGGAC1980.10068854693205048No Hit
TGTCAAGGGCGGGAGCTCAGGCTCCAAGTCGGGAGGCGACAAGATGTTCTCC1980.10068854693205048No Hit
ACCATTGGGAGGCTCCTTTTTGACCCCATCATTCCAGGCAGGGACCATCAAT1980.10068854693205048No Hit
CAAGTTGGGAACTTCTGAGGTTCTTGAAGTATTTCAGTTCATTTCTTGTCTT1970.10018001891724217No Hit
GCCTTTGGGTAAAAGCTATACTCCATCTAAAATAAGACATGCCTGAAATTTG1970.10018001891724217No Hit
TGGAGCGGGAATGTAAAATAATTTGGGATTATTTCAACTTTACATTGTATGT1970.10018001891724217No Hit
TATTTCGGGCTGTTTGAAAAAGCTAATTTATTGATTTTGAAAAGTTTTAAAA1970.10018001891724217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)