FastQCFastQC Report
Sat 31 Dec 2022
SRR5847117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198312
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT8650.43618137076929286RNA PCR Primer, Index 34 (100% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC8020.40441324781152926No Hit
TCATACGGGAGACTCTTTCCATACTGTAGTATTGGGAAGGAAGTCCCTATGT2960.14925975230949212No Hit
TGTCAAGGGCGGGAGCTCAGGCTCCAAGTCGGGAGGCGACAAGATGTTCTCC2650.1336278187905926No Hit
TGCGTCTGGGGAAGGGATTTTTTTAATGTCACGTGGCTCTCACAGGTGCGGT2590.13060228327080559No Hit
ACTAAGGGGCAGTCTTGATATTTTGCAACATTGCTGATGTGTGGATAATTTA2560.12908951551091208No Hit
CAATGCGGGATCTAAAAACACAAAACGTTAGGCCTTTTTCTTCTTGGGGACG2490.12555972407116062No Hit
ATGCTGGGGACAGCAAACCCCCATGACTTGAGTTTACCTATATAACAAGTCT2410.1215256767114446No Hit
GTACACGGGGTAAAGGCCAAGCTGATTGTCTCTGGGTTCCGACACATCTCCC2400.12102142079148008No Hit
TATCTAGGGTGCACTAACAGGTACCATGTAATATTCATTGCTCTCCCTGCTG2360.11900439711162208No Hit
GTCTTTGGGATTCTAACAATACACTATGTAGCAATGTGAAGTTTTATCCATA2350.1185001411916576No Hit
AAGACAGGGGATGTGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGG2320.11698737343176409No Hit
GTAACAGGGGTCAAGTGATTATATTGCTTGTTTTCAAGAAACCTTCAGATTT2320.11698737343176409No Hit
AGCTAGCGGGTGAATTAGAGTGAAGGAAAGCTGTGTGACACCTGGCATTCCT2310.11648311751179959No Hit
GCCATGGGGAACCATCCCTGCATTCCACAGGGGACTCGGGCAAGGGTGCCGA2310.11648311751179959No Hit
GTACCTGGGCTTCATTCCACTTTTACCATGTTTGTTTGCTTCCTTTTATGGA2300.1159788615918351No Hit
AAAGTAGGGCTTACTTTGCACCTCAAATTTCAGAACTCTAAATGTGCCCTGA2290.11547460567187058No Hit
TTTACAGGGTAACTAGCAATTCTACCCTAAATTGAGGTCCACTCTGAGAAAA2280.11497034975190609No Hit
ATGCTGGGGTGGAATCTCCATTGAAGAAAGGGTGAAGGAATAGGACTCCACA2270.11446609383194159No Hit
ACTGCAGGCGCGTGCCACCATGCCCGGCTGATTTTTGTACTTTTAGTAGAAA2260.1139618379119771No Hit
GGCTGCCGGGGTACCTAGTGCAGATTTGCAAAAAAAGAAAAGGAATAGTCAT2260.1139618379119771No Hit
CAACAAGGGGCTTGCTCTTAGACAAATTCTTGAAGCTTTTATTCCTCTTCTC2260.1139618379119771No Hit
CTAGTTGGGGGATACTTGAGTTGAGTTCTAAAAGGTGGGTAGAAATCAGCCT2240.11295332607204808No Hit
TTTGATGGGATCTCTAGCTCGACCCCAAACTGTATATTGATTTAATTAATAT2230.1124490701520836No Hit
ATAGTGGGGAATTAGACATTTGTGGGCACAGGGTTCTTTTGTTTTTGGGTTT2230.1124490701520836No Hit
GGTGCGGGAGAATTTCCCAGGGCACCTGCCGGCAGCTATCTTGATAGGAAAG2230.1124490701520836No Hit
TTCGCCGGGCAAACCTAAAAGTAGGTATTTGTGTAGACATAAGTTTACTATT2210.11144055831215458No Hit
GTAATGGGATCAGCTTAGAAGTTAAAATTTAGAAAAGCTGTAGTTCATTAGT2190.11043204647222558No Hit
CTAGCAGGGTTAATGGTTCCAATTTTATTTGCATCTACATAATAATTTTAAG2180.10992779055226107No Hit
CTCAGTGGGTAACTCTAATGGGATGGGATGAGAAGGGCATGCTGCCTGTCTG2180.10992779055226107No Hit
GAAATGGGGACTCATAACTTTTCTGCAAGAAATACAAACTTACATCTTCCTT2160.10891927871233209No Hit
TGTGGTCGGGTGATTTGCTAGTTGTACAGTTTTGTATGCTGATCAGAATTAT2150.10841502279236759No Hit
ATGACTGGGATGGGGAGAGGAGGACATTTGGGCTCACCTGTCAAGGTGGCCC2140.10791076687240307No Hit
AACAAGGGGCGATCTCCTGACCTCATGATCCGCCCATCTCGGCCTCCCAAAG2140.10791076687240307No Hit
TAAACAGGGGTGTTTTGCTTGTGTTTTTATAAGATATGGGATTTCTATTTGT2140.10791076687240307No Hit
ACTTGGGGAAAAAACTCACAGGTGAACCATGTAGCTTAGAAATATTGAAAAA2120.10690225503247408No Hit
CTTTCGCGGGCGATCTAGGCATGGGATAGACATTATGCAACGTTATATTTTG2120.10690225503247408No Hit
ATTGCCGGGTGCAATGCAAAATGTGTTTTCATAGGGAAAAAAGGGGGAGGAC2080.10488523135261608No Hit
TCTGGGGACACTGGAAATAAATAGGAATTTCTGACAGAAGAACATTATGGCC2070.1043809754326516No Hit
TCACAGGGAACTTTTCAAATTTACATAGACGTAATATAAGTAATACTGGAGA2070.1043809754326516No Hit
ACGCCTGGGGCTTTGGGTGGGCTCTGGCATCAGCACTTTCACTAAGCTTCAC2050.10337246359272258No Hit
TGGCCGGGATTCAAGTAGAGTAAAAAATAAGTTGAGTTATAAAGAAAAATGA2050.10337246359272258No Hit
TATTGTGGGCATCGAGGGATGGCATTACCTCCGTGTTTCTCCCTTTCGAGCC2050.10337246359272258No Hit
TAATTAGGGATTGGGTTTTAGTTTGCATTTCCCGAATTACTAATGATATTGA2040.10286820767275809No Hit
TTTGGCAGGGAAATAAAGTCTCTCTTGATAAGGTTTTTCTGTGATTTCCCAG2030.10236395175279357No Hit
TCACCTTGGGCGTACGGAATTAAGAAGGGTAAGGGGTGAAAATGAATGAGAA2030.10236395175279357No Hit
TAAGGTGGGGAGTTTAGCTTTGAGTTTGGGCCAATCCACAGATAGCTTAGGT2030.10236395175279357No Hit
AGTATGGGGGTGTCCAGCCCGGCTGAGCATCGCCAGGGCTAGCTCATGCTGC2010.10135543991286458No Hit
GCTGCTGGGTCAGAGGGTCATGGCACAGGCTCTGGGCATTCCAGCCACCGAA2010.10135543991286458No Hit
AAAAGAGGGGAGGCCCGTTTGGCGCCTTCACTCGGGAAGACGTCGGCCAGGA2010.10135543991286458No Hit
CCCACATCGGGTGGATTTCTGAAGCTCTCCAAGCCCTGCTGTTATATTTTTC2010.10135543991286458No Hit
AAACCTGGGTGGCTCTCTTCTCAGAATAAACCCAGAATCTGTCCAGTCCTTA2000.10085118399290008No Hit
CAAAGAGGGGTACATCAGTGATGTTAAAAGAAAACGTTTCCACATTTTAGGA2000.10085118399290008No Hit
TAACTGGGACTCTCCGTCGCGCTGCGCTGCGTCGCTTTCAGCCTCTGGTGAA2000.10085118399290008No Hit
GAACAGGGCAGTAAATTTTTACTCTAGTAATATTTAGAAGACACCATTCCAG1990.10034692807293556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)