FastQCFastQC Report
Sat 31 Dec 2022
SRR5847116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195358
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGC7800.39926698676276373No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGGCATCTCGTATGCCGTCT7740.3961957022492041RNA PCR Primer, Index 34 (100% over 21bp)
ATGCTGGGGACAGCAAACCCCCATGACTTGAGTTTACCTATATAACAAGTCT2570.13155335333080803No Hit
GTAACAGGGGTCAAGTGATTATATTGCTTGTTTTCAAGAAACCTTCAGATTT2530.12950583032176824No Hit
TCATACGGGAGACTCTTTCCATACTGTAGTATTGGGAAGGAAGTCCCTATGT2520.12899394956950827No Hit
ACTAAGGGGCAGTCTTGATATTTTGCAACATTGCTGATGTGTGGATAATTTA2410.1233632612946488No Hit
ATGCTGGGGTGGAATCTCCATTGAAGAAAGGGTGAAGGAATAGGACTCCACA2290.11722069226752936No Hit
AAGACAGGGGATGTGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGG2260.1156850500107495No Hit
CAATGCGGGATCTAAAAACACAAAACGTTAGGCCTTTTTCTTCTTGGGGACG2230.11414940775396963No Hit
TGTCAAGGGCGGGAGCTCAGGCTCCAAGTCGGGAGGCGACAAGATGTTCTCC2210.11312564624944974No Hit
GCCTTAGGGGAGCTCGAGGAAGTCACCCCATTAACTGACACACTGCTGTTCT2180.11159000399266986No Hit
TGTGGTCGGGTGATTTGCTAGTTGTACAGTTTTGTATGCTGATCAGAATTAT2170.11107812324040992No Hit
AACAAGGGGCGATCTCCTGACCTCATGATCCGCCCATCTCGGCCTCCCAAAG2170.11107812324040992No Hit
TAAACAGGGGTGTTTTGCTTGTGTTTTTATAAGATATGGGATTTCTATTTGT2170.11107812324040992No Hit
CAAAGAGGGGTACATCAGTGATGTTAAAAGAAAACGTTTCCACATTTTAGGA2150.11005436173589002No Hit
TATTGTGGGCATCGAGGGATGGCATTACCTCCGTGTTTCTCCCTTTCGAGCC2150.11005436173589002No Hit
TCTGGGGACACTGGAAATAAATAGGAATTTCTGACAGAAGAACATTATGGCC2130.1090306002313701No Hit
GTCTTTGGGATTCTAACAATACACTATGTAGCAATGTGAAGTTTTATCCATA2130.1090306002313701No Hit
ATTGCCGGGTGCAATGCAAAATGTGTTTTCATAGGGAAAAAAGGGGGAGGAC2120.10851871947911015No Hit
GCATATGGGATGATTTTCTGAAAGAAACTCCAATATGGCCGATTTTGAAATA2110.1080068387268502No Hit
TATCTAGGGTGCACTAACAGGTACCATGTAATATTCATTGCTCTCCCTGCTG2110.1080068387268502No Hit
ATAGTGGGGAATTAGACATTTGTGGGCACAGGGTTCTTTTGTTTTTGGGTTT2110.1080068387268502No Hit
GTACCTGGGCTTCATTCCACTTTTACCATGTTTGTTTGCTTCCTTTTATGGA2110.1080068387268502No Hit
GGCTGCCGGGGTACCTAGTGCAGATTTGCAAAAAAAGAAAAGGAATAGTCAT2110.1080068387268502No Hit
ACTGCAGGCGCGTGCCACCATGCCCGGCTGATTTTTGTACTTTTAGTAGAAA2100.10749495797459024No Hit
TATTTCGGGCTGTTTGAAAAAGCTAATTTATTGATTTTGAAAAGTTTTAAAA2100.10749495797459024No Hit
TAACTGGGACTCTCCGTCGCGCTGCGCTGCGTCGCTTTCAGCCTCTGGTGAA2100.10749495797459024No Hit
TTTACAGGGTAACTAGCAATTCTACCCTAAATTGAGGTCCACTCTGAGAAAA2090.10698307722233028No Hit
TATGCTGGGATTTTTAAGTATTTCACATGAGCAGAATCTGATAGTTTTTTGA2080.10647119647007033No Hit
AGTACCGGGCACCTGGATTTTCAGGACTTCCAGCTTCCTTGGCAACAGGTCC2070.10595931571781038No Hit
GAACAGGGCAGTAAATTTTTACTCTAGTAATATTTAGAAGACACCATTCCAG2070.10595931571781038No Hit
CTAGCAGGGTTAATGGTTCCAATTTTATTTGCATCTACATAATAATTTTAAG2050.10493555421329046No Hit
CGCCACGGGCATGTAGGCTTGAAAGGCAAGACTTTTTGGAAGAGCATGGTTT2050.10493555421329046No Hit
TAACTAGGGGCAGATTTATGCCTTATTTTTTAGCATTTTTTAAGGTTGGGTC2030.10391179270877056No Hit
CGCATAGGGGTGGCAGAAGGATGAATTGCGGGAGACTCTCAAATCCCTAAAG2030.10391179270877056No Hit
GCCATGGGGAACCATCCCTGCATTCCACAGGGGACTCGGGCAAGGGTGCCGA2030.10391179270877056No Hit
CATTGAGGGGATGAATGCATGGATGGGTGGATGAATAGATGGATGGATGGAC2030.10391179270877056No Hit
AACAGCGGGTGACAGCTGACAGCTTGACAGGCACTGCATCACCACAGGATGT2030.10391179270877056No Hit
GGTGCGGGAGAATTTCCCAGGGCACCTGCCGGCAGCTATCTTGATAGGAAAG2030.10391179270877056No Hit
CGTCGGGGGCCAGCACATTTTGCAGGAGTAAATGTCAAAAAGACTAAGTGTT2010.10288803120425066No Hit
GCTGCTGGGTCAGAGGGTCATGGCACAGGCTCTGGGCATTCCAGCCACCGAA2000.1023761504519907No Hit
AAAAGAGGGGAGGCCCGTTTGGCGCCTTCACTCGGGAAGACGTCGGCCAGGA2000.1023761504519907No Hit
GAGGCGGGGATTATTTGAAGATGGTGGAGTCATTAGTTAAGATGAATTTGAG1990.10186426969973075No Hit
GAGGCTGGAGTGCAGTGGCGTGATCTACGCTCACTGCAGACAAATATTTTAA1990.10186426969973075No Hit
TCACCTTGGGCGTACGGAATTAAGAAGGGTAAGGGGTGAAAATGAATGAGAA1980.1013523889474708No Hit
ATTGACGGGATAGCTTTTGAAGAAAGGCCAGTGATCCAGCAAAGGGGGAAAA1980.1013523889474708No Hit
TTCGCCGGGCAAACCTAAAAGTAGGTATTTGTGTAGACATAAGTTTACTATT1970.10084050819521084No Hit
GGGTCTGGGTCCCCCCAACCCCAAATTTGTCTAAGCACTGGCCAGTCTGTTG1970.10084050819521084No Hit
CCCACATCGGGTGGATTTCTGAAGCTCTCCAAGCCCTGCTGTTATATTTTTC1970.10084050819521084No Hit
CTTTCGCGGGCGATCTAGGCATGGGATAGACATTATGCAACGTTATATTTTG1970.10084050819521084No Hit
GTACACGGGGTAAAGGCCAAGCTGATTGTCTCTGGGTTCCGACACATCTCCC1970.10084050819521084No Hit
CGAAACGGGGGTGGATGGGGAATGTAATTGATCGATCCTGATCACTGACTCT1960.1003286274429509No Hit
TTGCACGGGGCCTTAGTGTCAGCCTGAAAATACCTTTGGGGTCTGTGAAACT1960.1003286274429509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)