FastQCFastQC Report
Sat 31 Dec 2022
SRR5847086.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847086.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66290
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACAGCGAAATCTCGTATGC2030.3062302006335797No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGCGAAATCTCGTATGCCGTCT1680.2534318901795143No Hit
TTAAATGGGCCGATCTCTCTAGGGGGGAAATTTTTAGCACTAAATGCCCACA830.1252074219339267No Hit
ATACATGTATTTATTTACACAGGCAAGCAAACAGAAATCCATGGGCAACATT810.1221903756222658No Hit
GGCGCTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA770.11615628299894404No Hit
TGAACAGGGGGTGCAATAAACTTATAACTTTAGAAGATTTTTGTGAGGGAGC760.1146477598431136No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC730.11012219037562228No Hit
GCAATTGGGAGATTGATATTGCCTGTTGGTCCTGCAGGCGGAAACCAAATGT730.11012219037562228No Hit
GAGCTAGGGACTCTGTGTGCCTCTTCTACATTGCATTCTGCTCTGCTATGTT730.11012219037562228No Hit
TTAGTGCGGGGTAAAAAAATGAATCTTTGTAATTACTTAATATGTTAGTTAA730.11012219037562228No Hit
AAATCGGGGATGTGGTTTAGTTTTCCAAACATGTTATCTTAAATACCCCTTT730.11012219037562228No Hit
ACAAGAGGGGTATTTGATTGATGGAATTTTGAAGTAAATGTGTTTTTATCCA720.10861366721979183No Hit
GGTTACGGGATGAATGCAGATGATACCAGTAAGTCAGCTTGTTCATTTGCTT720.10861366721979183No Hit
TTCGCTGGGATGGGGAAGTGGTCCAGAGCGGCAACACCAACCAGATGGTATT720.10861366721979183No Hit
GTGGACGGGGAGGCGGGAAGAGGCGGTGCCGGGGGCGCTGGCGTCCTGCAGT710.10710514406396138No Hit
GCATTGGGGCGACGTTCTTTTGACCGAACTGAGCAACTGCACGGTCAGACTG710.10710514406396138No Hit
TCTAATGGGATTGTTGATTGCCTCATGGTGTGTTTCCATTATTCAGTGCCTT710.10710514406396138No Hit
TTTAAAGGGTAGTGGGATGGCTGGGTCATATGGGTAGTTCTATTTGTAGCTT710.10710514406396138No Hit
GGCCAGCAAAATTAGCTCTTTTTCTCCCAGAGAATACTATCTTTGCATAGAG700.10559662090813093No Hit
AGTTCCGGGATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTG690.10408809775230049No Hit
TTAAGCGGGGTATGTGCATTTGGTATCATGGGCCGTGGAACAGAATATATGT690.10408809775230049No Hit
TGCTTTGGGAATGTGTTTTTATCCATCAGTTTCTGAGTAACAAAGAGCACCA690.10408809775230049No Hit
TTAGCCGGGGAATGAACGAATGAATGTACTATGAGCTGATACTGGGATTTCA690.10408809775230049No Hit
TAGATTGGGACCTGTGTCAGAGGACAACAATGTTATTACACACAGAAGCTGC690.10408809775230049No Hit
TTCGCCGGGTGTGTACTAAAACTACAAAACAAAATGGAAAGAAATTAAGACC690.10408809775230049No Hit
ACTCGTGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT680.10257957459647005No Hit
AGCTCCGGGTGGCAATGATCAGGCAGCCCAAAAGATTTAAATGATCTATAAT680.10257957459647005No Hit
GGCTGGGGGAGTGGGAGGATTGGGAATGACAGCTAAGGGGTACCGGTTTGTA680.10257957459647005No Hit
ATCGCCAGGGTAGTTACTGTTAGGCAGTATTCAGAGTTTTGATCTTCACAAT680.10257957459647005No Hit
GCCACTTGGCCTTTTTGAACCCCCATTAGATGGTGAGCATTTTCCTTATCTG680.10257957459647005No Hit
GTAGTGGGGATATTTGTTTCTGTATGCATTGTCCCCCTCCAACTTTGTTTCT670.10107105144063962No Hit
AGTTTAGGGGGAAATCCTTTGATTTTTGACTTTAGATGTTGACTTTGAAATA670.10107105144063962No Hit
TTAGAGGGGGTGTAACAGGACTGTTAATGGAATCGGGTCATTTAGAAACAGT670.10107105144063962No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)