FastQCFastQC Report
Sat 31 Dec 2022
SRR5847059.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847059.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104361
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAGCGAAATCTCGTATGCCGTCT6490.6218798210059313No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACAGCGAAATCTCGTATGC5360.5136018244363316No Hit
CACAATGGGGCTGATGTATAGAAACTATATTAAACTTCCAAAGAAAGCACGT1670.1600214639568421No Hit
ACTCTAGGATTCTCCACAGGAGTTCAGCTCAAATGATCATTTGAAAAATGCT1410.13510794262224393No Hit
GCAAAGCGGGACGTTGCTTCGACAGCAACTGGATGATGCTCACAAGAAAGCT1380.13223330554517493No Hit
TTGCAGAGGGCATGTTTCTCACATTGCTGTATTTTAGAAAATAGGGGTTAAG1330.12744224375005989No Hit
TCCTCTGGGGGAAATGTTTCACCTTATTTTTGGTCTTTGTTTATTCATTTAG1310.12552581903201387No Hit
TCGTTGGGGATTCCGTAACCCAGATGATGTCTTCAGGGAATTTTTTGGTGGA1300.12456760667299087No Hit
GATTCTGGGGAATATAGAACACAGAATGTGGCACGCTTGCCCATCATAGATC1260.12073475723689885No Hit
TGATTTGGGGTTGCTGTTTGTATTTCTTTCACAGTTTTGATAATATCACATC1230.11786012015982981No Hit
GAATAAGGGGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT1220.11690190780080682No Hit
CTGCTTGGGATACAAATAGCAAACAGGTATATAAAAATGTGCTCAACATTGA1210.11594369544178382No Hit
GATACTGGGATATTCTAATGGGGAAGACCAACAATAAATGAAGAAGCAAACA1180.11306905836471479No Hit
GCAGCGGGGATCCAAGAAGCACGTTTGATTCAACACTGCAGGTCATACGGGC1180.11306905836471479No Hit
GCGCTGGGATCGCCAGGGAGCTGGAGCGTGTGGAGCAGCAGTCTCGGCTGGA1170.1121108460056918No Hit
AAGCACGGAGGAACTGTAAGCTTTTATCTTTTAACCAACTGAACAATACACC1160.11115263364666877No Hit
CATCAAGGGGAGGCAGTTCAAATTCTCACCACCTTCTTTTCTCTCTTACAGC1160.11115263364666877No Hit
ATTTCAGGGCTTATAGTCCAGGGCCTGTTTCCCTGTAGCAGCTCCTTATTGC1150.11019442128764577No Hit
GGACTGGGTGGCTCTGGCTGCTTTTTAGAATAATGCAAGATAAAAATCACAC1140.10923620892862276No Hit
TGAAATGGGTGGCAAGGAAATATGTTTAAAGCCGAGAACTAAAATAGCTTTT1140.10923620892862276No Hit
GGCACTGGGTGGGTGACGCGCAGCACGGCGGGCCTGGGCCTGGTGTGTGGGC1140.10923620892862276No Hit
TTTACTGGGTGTTGCGTTAGGGAGAATTGAGGATTGTGTGAAAGTTACCAAA1140.10923620892862276No Hit
AGTGATGGGTAATTTCTATTATTCCAAGTTCTTCGATTTGATTTGTCTAATG1130.10827799656959976No Hit
AGCAAGGGAGAATGGCTAATACTAAAAGGTTTAAAAATAACAGATGCTGACA1130.10827799656959976No Hit
GCCAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1120.10731978421057674No Hit
CACACAGGGGATTTTTCATCACTTTCTCTTTAGAAAAAAAGTACTACCTGTT1120.10731978421057674No Hit
AACTGAGGGAGTATTACAAAGAAAAATGCCCAGAAACTTTGATTTGAGTTGA1120.10731978421057674No Hit
CGTAAGGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1120.10731978421057674No Hit
CGTACAGGGAGTTCAAAATATTCTGGCTACCATTTGATTATTTTCTGTGAAA1110.10636157185155373No Hit
ATCTTAGGGATGCCAGAGAGGAAACTATAAAGTAATTACACATGTAATCTTG1110.10636157185155373No Hit
CACCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1110.10636157185155373No Hit
CATAATGGGGAAGAAACAACATGATTTATTAAAAAGAATGTTACAACAGCAG1110.10636157185155373No Hit
GATGTAGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG1110.10636157185155373No Hit
ACGTCCGGGGTACCACTTCATTTGAGGTAGGAAATTGTTGCAAGTGTCATGC1110.10636157185155373No Hit
CATCACATTGCTTATCTGTAAATTCCCACTCCGAAAGTGAGAAGACTGGATC1100.10540335949253074No Hit
TTAGAAGGGGGTTCCATGTTTTCATTGTAAGAAAGCAGAGAGCGGAAAATCA1100.10540335949253074No Hit
TTGCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1100.10540335949253074No Hit
GTATCAGGGACTTACCATGTGACTTTTTATTACCAAATGCATCAGTAGTGGA1090.10444514713350773No Hit
AATTGCGGGTATGTGCTTCCAAGTTCTGCTTGCTTTAGAAATCTTTACATTT1090.10444514713350773No Hit
CGAGTTGGGAATGGAATGAAACTAGAAATCAATAACAGAAGGAAAACTGGAA1090.10444514713350773No Hit
TAGTATGGGAGTGAATATTGCCATTATTATAAAATATCTATTTACTAAGCCT1090.10444514713350773No Hit
TTGCCTGGGGGCCAAGATGGCGGCAGCTGCGGCTTCGCTTCGCGGGGTAGTG1090.10444514713350773No Hit
AATGGGGGGGTGGTGGACCATACGGTCTGTGTCACAACTACTCAGCTTTGCA1080.10348693477448472No Hit
ATGCAAGGGCTCATCTGGTAGTCCATTACTCTTCTCAGTCTATTTTTACATT1080.10348693477448472No Hit
GACGATGGGAGGGGAGTCTTCTAAGAATTAATAAATCATAACTTGTTTATGA1080.10348693477448472No Hit
TCTTCAGGGAGGCGAGTCATACTCTTGCAAGTAGTTAGCAAAGGGAGGCCCA1070.10252872241546171No Hit
GAACGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1070.10252872241546171No Hit
AAGCTGGGGATTCAGGAAATACAGAGAACACCACAAAGATACTCCTCAAGAA1070.10252872241546171No Hit
TGTCGAGGGGACTGATACATGAATTGGGGGTCTGGCATATATTCTGTTTAAA1070.10252872241546171No Hit
ACAATTGGGTCATAGCCCATGTATTTACACTGAAAATGTTTTATCTTTTTAA1070.10252872241546171No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGCGAAATCCTGTCTCTTATAC1060.10157051005643872No Hit
CTATTGGGGGAGAAACTACAAAAGAAAGTTGTCCTCGTCCTTCCCCTTAGCT1060.10157051005643872No Hit
ACAAATGGGTAAGCCTTCAGTGAATTATCAAATGCAGCTGTGGGCCAATTTA1060.10157051005643872No Hit
ACACTAGGCTGCCAAATCTGCCTGGTTATGTTCAGCCCATGAGCTAAAAATG1060.10157051005643872No Hit
TAAATTGGGCAGTGCTGAGAGTCATCTTTGAGTTCTCCATTTCACTCCCTAC1060.10157051005643872No Hit
AGAAACGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA1050.10061229769741571No Hit
GCATTGGGAGTGCATTTTTTGTTCTATTTTTAGTGTAGATTTAAGCACTTTG1050.10061229769741571No Hit
TTTGGCTGGGAGGCATTTGGTTTGCTTCCATCTTTTTTTATGCAGTGCCTGT1050.10061229769741571No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)