FastQCFastQC Report
Sat 31 Dec 2022
SRR5847054.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847054.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150926
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGC8500.5631899076368552No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGCCGTCT6400.4240488716324557No Hit
TGCAGCGGGAACTCAATCACAGACCCAAATATTTTCAGTAGGCAATGGTTGT2420.1603434795860223No Hit
AGCTATGGGTGGGAGTGCCCTGCAGAGTCGCTGTGCTCAGGTGCTTTCTGTG2360.156368021414468No Hit
GCAAATGGGGGAATCGGTTCCCGGGTGATCCTCGCGCCTGCCGCTGCTCGGC2190.14510422326173092No Hit
TCGACCAGGGTAGCTTCTTTGGTGGCGAACTTGGATTGTGATGCCCTCAGGA2180.1444416468998052No Hit
GCACTAGGGAGACTTGATGATGAAGTAGGTGGTCCTGAAAGTTAACAGGAGG2140.14179134145210237No Hit
TTTGAGGGGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAATATC2050.13582815419477093No Hit
ACTAAAGGGATTCCCTGCCTGTGCAATCCTTTGTATTTCCTCCATGCCTTAT2040.13516557783284522No Hit
GATATAGGGACACCCAGAAGACGTCAGGTGACTTCAGTCCTGCTGCAGTTGT2030.13450300147091954No Hit
ATTGTAGGGTTTCTTGTATTTCTATATACAAATAACAACCTAGATGAAAAAT2030.13450300147091954No Hit
CAACATGGGACGCTCTTCCGATCTCTACTGCCGGGAGCTACATAGGGACTTG2010.13317784874706812No Hit
ACTATAGGGACTTCTGACATTTGTAGACTCCTGGCGGGTGCAGGGAGGGGCA1980.13119011966129096No Hit
GCAATAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1960.12986496693743954No Hit
AGCCAGAAACAGACCATGAAACAAACAAGCAAACAGAAAAGCAAACACTCCC1940.1285398142135881No Hit
ATCTACGGGGTGAGATAATGAGAGAAGGCAGTGCTTTTGAGGATGACGACAT1930.1278772378516624No Hit
AGTATGGGAGATGGAAAATAAGAAGATGCACTTTCTGTAACTTTGTCTAAGG1920.1272146614897367No Hit
AGCTGGGGTGGGCAGGCGGGCGGCTGGCAGCTGTGGCGCCGACATGGCTGCG1920.1272146614897367No Hit
GTAGTGGGGAAGTAGTGGCTTTCTGGATGGTAATTCCATCTTAAGGTGTCAG1920.1272146614897367No Hit
GCAGCTGGGATGTGAGTCCAACCGTTCAAGGCAGCAGTAACATGCCTTTATC1890.12522693240395955No Hit
TAATACGGGCATGAGATTTACTTCTATCATATAAGTACTGCTAGTAAAACCA1870.12390177968010813No Hit
GGCCTGACGCCTGAGCAGCGAGTAGACAAGATTTTCAGCAAGATGGATAAGA1860.12323920331818242No Hit
GTAACAGGGCGATCTTGAAGCGGGTAAAAGTGGCCTTTCTGTTACGTCTGTG1860.12323920331818242No Hit
CACACAGGGGTGGGACACATGATTACGTATTGTGCACAGGTTCACTTGGTAA1860.12323920331818242No Hit
GATCGCGGAGCCGGTTCAGGGGCTGGCGCGCCAAGTCCGGGATATCCGACGT1860.12323920331818242No Hit
ATTTGTGGGCCTTAAGCGTTTGAATGTAAATACTTCTCCCCACCCCGAATAA1850.1225766269562567No Hit
GCTCTATGAGGTATGAGCCAGGGATCCTTTCCTGGTCCCTAAGATCAAACCC1830.12125147423240527No Hit
TCCAATGGGGAAATACTTAATTGTGCAGGTTGTAAAAAAGATTAGTGCATTT1830.12125147423240527No Hit
AGTGGGTGGAGAGCATGGGTGGTAAAGTACCACCTGCTACTCAGAAAGCTAA1820.12058889787047958No Hit
GTATATGGGCAGATGTCCTGAGTGTGGGAAAACCTTCTATGAAAAGTCAGCC1810.11992632150855387No Hit
ATGCCAGGGGGAACTGTAAGCTTTTATCTTTTAACCAACTGAACAATACACC1810.11992632150855387No Hit
GATGCAGGGGATGAGTTTCGTGAAGCGATTTCCTGCCGCTGATTTAAAGTGT1810.11992632150855387No Hit
GATTTAGGGAAGCAGCTTCGTTATATATTTTAAGAATTGGTGGCTGACCATG1810.11992632150855387No Hit
GGACATGGGGACTGTGCCCGGAGGGAGGCGGCGGTGGCCAGTAATGCCTGGG1800.11926374514662816No Hit
AGAAAGGGGGATCTTCTTGTGGGAGTGGAAGGAGGAGGGGCGAAAGTACGCC1800.11926374514662816No Hit
AGTAGTGGGCTTATTTCAACTGGTTATTGTAAACGCTGCTTACTAGAGACAT1800.11926374514662816No Hit
TAACTTGGGTCTTGAAATAACCAGGCGATACTTTGTTTTGTATATATTTGTG1800.11926374514662816No Hit
AACTCTGGGATTGGGAAGTGTGTGATTTCGGCAGACCTGCTGGGAAAATTTA1780.11793859242277673No Hit
TGTACAGGGGGCCTTGGAGGCGATGGCCCACGGGGCTGCTAGCCGTGCTGCG1780.11793859242277673No Hit
CTTGCTCGGGATTCAAATGCATATAAAATACCACCATGTTACACTGTTTTCT1770.11727601606085102No Hit
AAATGCGGGATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCATATATTTA1770.11727601606085102No Hit
GCAATGGGGAGGGGGAAAACAGTAGTTCCGATTTTTTCCCATTATTTTTATT1760.11661343969892529No Hit
AAACACGGGCTATCAGCGGGACTGACCCATATTATGTACAGCATTTCTGATC1760.11661343969892529No Hit
CGCAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1750.11595086333699958No Hit
TAACTTGGGTGGCAGAGATGGTTTTTTAATGAAATCCCACCATCTATCTGAG1750.11595086333699958No Hit
ATTACTGGGAGACAGCCAGGGGGATGGTGCAGCTGTGAAGCTCGGGCTGATT1750.11595086333699958No Hit
CTATCAGGGACTTCCTGGAGCATGGCATCCCCCCCAGCTGCTGCATGAACGA1740.11528828697507387No Hit
GTCACAGGGTGCTCTGACATGCTAACATTTCTTTTGTTCATCCCTGTTGCCC1730.11462571061314816No Hit
AGCCAAGGCGGGTGGGTCACCTGAGGTCGGGAGTTCGAGACTAGCCTGACCA1730.11462571061314816No Hit
GTTACTGGGATCATGATGGAGACTTTGGAATTACGCTCGCAGAGCTGCGGGC1730.11462571061314816No Hit
AATGCTGGGGGAAATGCTGTGAAGCATCAGATGACCCATCACTCTTTTGCCT1720.11396313425122247No Hit
CAAACACGGGCGATCTCAATAAGGGGCTGCTAGAGTCTGTGCCGTGCTGCCT1720.11396313425122247No Hit
GGTGCTGGGGGGTTCAACCATGATCAAAGAAAAACTGAGGTCACCTGCAGGC1720.11396313425122247No Hit
TTATGAGGGATATGGCATTTTCTAACTTAGGAAGCCACAATGTTCTTGGCCC1710.11330055788929674No Hit
CAATTCGGGCAAATACAATGAATATAGGCAGAGCATATAGAGTTTGTAGATT1710.11330055788929674No Hit
GTACTAGGGCAGGTACATCTGTGAACACAACTGGCTATTGGAGGAAGCAAAA1710.11330055788929674No Hit
TGGCGGGCATGAAAGCTGATGCCAAGGCCAAAAGAAGTTTAAAGCATCTGTA1710.11330055788929674No Hit
TTGAAAGGGACCTCCCAACACGACCCAAGAGCAGGTTTACAATGCATGTGCG1710.11330055788929674No Hit
TATTTGGGTATAAACTCAAATCTATTTTATCCTTCATCTGTTTGGATTGATG1700.11263798152737103No Hit
CGGCAAGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1690.11197540516544532No Hit
CCATGGAAGGGTATAGATACCACCGTGTAATAGAAGACTTAAGTCAATGAAA1690.11197540516544532No Hit
GCAGTGGGGTATATGTTTGTAATTCCATAAAATTGGGATGATGAATATAATT1680.11131282880351961No Hit
TTTAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1670.11065025244159389No Hit
AGGCATGGGCCCACAGAAGCAGAGTTACAGGACATGATTAATGAAGTAGATG1670.11065025244159389No Hit
GGCTTGGGGTGCAATGTTTTACTCACAATTTGCACATTCACATTTTTTGGAC1670.11065025244159389No Hit
AAAGTAGGGTGTATCCAGGAGAGGAACTGCATAACCAAAGGGGTGACCAGCC1670.11065025244159389No Hit
GCCAATGGGTACCTTGCAAAGGTCCCATCTTCAAATACTATCGTATTGGGAC1660.10998767607966818No Hit
ACCTCAGGGAACTTAGAGAACCAAACAAAACACTTTTTTGTTTGTCAAGGAT1660.10998767607966818No Hit
AATGCAGGGCGATCTTCGTGTGGGGATGAAGTAACATGATCACATCATCAAC1650.10932509971774247No Hit
GAGTTAGGGTCAGTGGTGCTAGTTTCTCGGGTCTCAAGGTCGTTATATTGAG1640.10866252335581675No Hit
CTGTCTCCCCTCACTTGTCATATGCTCTGACATGCTAACATTTCTTTTGTTC1640.10866252335581675No Hit
TAAGAAGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1640.10866252335581675No Hit
GTCAATGGGAAAGGGCTGGACTACTATAAAAGTTACAAATACGTAGTTAGAC1640.10866252335581675No Hit
CATTATGGGTGGTGACTGGTATCAAGGTTGTCGATCTGCTAGCTCCCTATGC1630.10799994699389104No Hit
TTAGGGATGGGAGAACTGGGCTGTGACAGGCAATCCGAAGAGCAAATGAAGA1620.10733737063196533No Hit
ATCTAAGGGAAGACTATAAGTTTCTATGATATTTTCCCCAGGATTCCATTTT1620.10733737063196533No Hit
GGTGTTGGGTAATTCTTGCCCTGCATCATGCATTTGGCAATATGTCACATAG1620.10733737063196533No Hit
TTATGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1610.10667479427003963No Hit
ACTTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1600.10601221790811392No Hit
GTGCAGGGAATGCAAATCTGGCTCAGCATTCAAAAATTGATATATCCCACAA1590.1053496415461882No Hit
TGACAGGGCAACTTAAAAGTCAGTAGATACATTCATCCCTAACAAGAGTCAG1590.1053496415461882No Hit
TGTTAAGGGACTTGTAAAGCCTTTTACACCAAGTGTCAAGGAGATTTCTAAT1590.1053496415461882No Hit
CCACCTGCTCACCGCCAGTTAGCTTTTCCCAGGATGGTGGTGGCACTGATCA1590.1053496415461882No Hit
CTCGCAGGGTCTTTGGGAGGGGATTGATTTATAGTTCTAAGAGATTTTTGTT1580.10468706518426249No Hit
AAACGCGGGAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAGGAAGGAA1580.10468706518426249No Hit
GGTTTTGGGGGGATGTCGCGGTCTCGTGGTGGAACATCTGACCCTTTTCAAG1580.10468706518426249No Hit
TTAGAGGGGACTGATCCTAGCTCTGGCCTGTGAACTATAGTTTTCATTTTGC1580.10468706518426249No Hit
TCCGAAAGGGTGTGTGGAAATAATGTAGCTGTCTATTGGATGCCACTATTAG1580.10468706518426249No Hit
CATAAAGGGAAACCAGATATATGACTTGCAGCACTTCTAAAAGATTGAAAAC1580.10468706518426249No Hit
TTCGCCGGGGTAGAATGGAAGGCCAAAACTGAAAAATTCTGGCAAATTGCCC1580.10468706518426249No Hit
GACTAAGGGGAATGTTTTTGTGGAAGTTCTGTAACTGTACCAGAAATTGAAG1580.10468706518426249No Hit
GGCTACGAGAGAGAGACAGGGGCAGTTTGAAGCTTTTATCTCCCTCTTCACT1580.10468706518426249No Hit
AAGACAGGGCTGATAATTTTCTGATCCAGAAAATACCAGCAGCTGCCAAACT1570.10402448882233678No Hit
GGTATTGGGGTATAAGAAAAATAACCATTGTGGGGTTTTTGTGCATCTTATA1560.10336191246041106No Hit
ACGACAGGGAGAAATGTTACTGGGAGGAGGAAAGGAAAATCACTTTTTTTCC1560.10336191246041106No Hit
ATGCCTGGGTATTCTGCATCAGTTTGCAGTTCACTGCAAATCTTTTACATTA1560.10336191246041106No Hit
GCGAATGGGGTTATTGCTCTTCTCTGTATATCAGATATTACAGTAGGAACTG1550.10269933609848535No Hit
TTACAAGGGCTGTGTGCAGACTGCGCCATTGGTGGGCAATAGCGTTGACAAC1550.10269933609848535No Hit
GACTAGGGGGATACCCTAAATAACCATAGTTGTCAGTCATTCTTTGAAGTTA1550.10269933609848535No Hit
AGTTTTGGGGAAATTTCCCTGACCCTAAATAATACATTTTGAAATGAAACAA1540.10203675973655964No Hit
TTTAAGGCGGATTGAGTTTACCTGATTAAACTTTAAAAGTTAATTGTAGATT1530.10137418337463391No Hit
GCCTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1530.10137418337463391No Hit
GCTATGGGCGATCTTTGCCTGGGGTGCCATAAAATTTCAGTAGCTACCTATT1530.10137418337463391No Hit
CGACCTGGGATGTCTTTCCGATGGTGTCTCCCAAGTGTTGGTGCTTTGGGTT1520.1007116070127082No Hit
TTAGAGGGGAACAAATGAAAGTTTGCTGTGTGATTGCAGTTTTAAATTATAA1520.1007116070127082No Hit
CGTTGGGGGTGTACCTATGCATTTTCTGACTTTCTTGTTCTTTAAACTTCAT1520.1007116070127082No Hit
ATCAACGGGTTATATTCTGAAATTTTGTGATTTACTTATAAATCTTCAATGG1520.1007116070127082No Hit
TTAGGACGGGTATCTATATATCTGTTTGATTATTTCAGGATCTGAGCAAATG1520.1007116070127082No Hit
GCAGTTGGGATGTGGTTCCCTGTTTTGACATTGCTTCCCTCTTAGCCATTTC1510.10004903065078251No Hit
ACCGCAGGGATGTTGGTTAGGGCAAAATACTAAGTATTCACAACTGTAGCAA1510.10004903065078251No Hit
CATGACGGGATGGAAGGCGGTTATAATGGATGGTGGTGTGTACAAGATGGCA1510.10004903065078251No Hit
ATAATAGGCACCAGGGCAATTGGGCAAGAGAAAAAAAATAAAAATATCAAAA1510.10004903065078251No Hit
TGCTGAGGGTGCCCACAGGGTTCTTCCCACTTTTCTCTACCCCTGTATTTCG1510.10004903065078251No Hit
CAACATGGGAACGGCTTTTAGTCTGGAATTCTTTGGAAAGAAAATAGACTTC1510.10004903065078251No Hit
ATTGCCGGGGGAACTCATTATACCTAAAGATAATACAGAAAGTAAATATATT1510.10004903065078251No Hit
CACTAGGGGTCTGGATGGAGGGCAAGTTACCATGCACTCCCAAGATGTACGA1510.10004903065078251No Hit
GCGTATGGGAAGCATGTCCACTACCCTGACAAGCCACTTAGTTAAAAACTGC1510.10004903065078251No Hit
GTGCGTGGGATTGCTGAAAGATATTAAAGAAGACACAAATAAATGGGGGAAA1510.10004903065078251No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)