FastQCFastQC Report
Sat 31 Dec 2022
SRR5847049.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847049.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140366
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGC5430.38684581736317913No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGCCGTCT3270.232962398301583No Hit
ACTGGTGGGGATCTAACTGAGGGACAAAAGGAAATGAAAAAGGAACTTAAAC2360.16813188378952168No Hit
ATTGCAGGGAGAGGTGGAGTATGAACCCAGGTGTATCTCTAAAGACCATGAC2000.14248464727925567No Hit
GCGCAGGGAATCCTATGGGCTTGCAAATAAATAACTTACTTGTTCTCTTCAG1900.13536041491529288No Hit
TAATTCGGGAGACAGACTGGTTGGATGGCAAGCATGTTGTGTTCGGTCACGT1800.1282361825513301No Hit
GCAACCGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1800.1282361825513301No Hit
AAAAATGGGTAGGTTTTTTAAACAGAATCCTACTGTTAATGGTAATAACTTC1800.1282361825513301No Hit
CATTTGGGAGGAAGAAATGTGGTAAGACGTATACTTACATTTTCCTAAGACA1790.12752375931493382No Hit
CACTTCGGGCTTTTTGCGAAGTGGGCTCGTGGGTTGGCAGTATGAGAGTTGT1780.12681133607853753No Hit
TGATTAGGGAGCGGCTGCAACTGCGGCTCCTGGCCAGACCCCGGCCTCAGCG1780.12681133607853753No Hit
TAAACTGGGACCAGGGAATTCTTACTGGCAAAATGATCAATCTGGAGTGTGC1770.12609891284214125No Hit
CTGCCTGGGACCAGCTGTGGTGGCTAAAGGGAATATGTTAATTAAGCAAGAG1720.12253679666015986No Hit
TTCGCCGGACGATCTATGCTGGGGAAGTAAAATGTGGAAAGAATTTAAATTA1720.12253679666015986No Hit
TTCGCTGGGGATACATTTTATCCTTTTCTCATTTGCTGGGTGGGGGAAGTGT1700.12111195018736731No Hit
TACTTAGGGACCTTATGCCCTGTTGAATTTTTGGTGTGAGATTTTTGAGTAA1680.11968710371457476No Hit
TGATGTGGGGTGGGATGTGAGAGTTGGAAGACAGCTTGCGGGCCTGCTCCGC1660.1182622572417822No Hit
GATCTAGGGTAGTTTCATCACATTTACTTCATCATGTTCACTTGTAAGCCTG1660.1182622572417822No Hit
TAAGGGGGCCAGAAGTCACTTCTTACTTTGACAAATACTGCAAAATTTTTCA1660.1182622572417822No Hit
GCCTTGGGGAGGGGTCAAGAATATAAACATACTCAATTGCTACAGCAGGAGG1640.11683741076898965No Hit
CTGAGGGACGAGGATGAAGTAGAGGATATGGAAGAAAATTAAGTGTGATCCA1640.11683741076898965No Hit
GTCTATGGGATATCAGACAAAACGGACTTTAAGACAACAGCAGTTTTAAAAA1630.11612498753259336No Hit
GCAGCGGGTAGACATTTTGCAGTTTGTTTAATAACAACTTCTAAAGTAAGTT1630.11612498753259336No Hit
TTTATAGGGGACTTCTCGGTGCTAGTTGGCCATCTTGACTCAATGTTGGGAT1620.11541256429619708No Hit
AGTTTAGGGAACCACAGCAGTGCTCAATTGTGGCGAATTACTTCTCTATCCC1600.11398771782340453No Hit
CTCGTCGGGGTAAGGGAGGATCATGTGTATTCTTAAGTGTGGGTCCTTATTA1600.11398771782340453No Hit
TGAGATGGGACGTGATAATGACTTACTGTTTTGTTTTCATTATTTCCTCCTT1590.11327529458700825No Hit
TATTCTGGGATTGAATGTAAATGAACTAAACTCTCCAATTAAAAGACAGAAT1580.11256287135061196No Hit
ACGATAGGGGGTGGTCCAAAGAAAACAAAATTGGGTTGAATGTTTCATTTAG1570.1118504481142157No Hit
AGCAAGGGGGGCAGAAGGGCAGAGTGTGAAGCGATTGCTCACAATGGCAATC1570.1118504481142157No Hit
ATCCTAGGGGCTTGGGAAGATGGCGGCTGTGTCGGTGTATGCTCCACCAGTT1560.11113802487781942No Hit
CACTACGGGCAGTGTGAAATTGGACAAAAGTGAAAGCCACAAAACAAACTGG1560.11113802487781942No Hit
TAATAGGGGCCCCTGAAGGATTTATTATAGCAGCTAACTTCTAACATCCATA1550.11042560164142314No Hit
AGACTAGGGTCTCCCTAGTGCGGGCTGGCAGTGCGGGCAGAGCCCGGCTGAG1550.11042560164142314No Hit
ATAGTAGGGTGACTTATTATAAATTTTAGAATGTATTTCTATAGGGTGGAAA1540.10971317840502687No Hit
TGGCGTGGGAGATTGGCAGTCTCTTTCGCTTATCTGCTGTTTTAAAGAATTC1520.1082883319322343No Hit
ATAGCAGGGTACTGCTAGATATGATTGTACAAGAATCTCCATTCCTTACAAT1520.1082883319322343No Hit
GGACATGGGCAGGACTCACATGGATATAGTTTCTGTCTCCCTCTCTAATTGT1510.10757590869583802No Hit
CTCACAGGGAGTACTTTAGACTAATTGTGTTTGTCCGTCCATGCCATGAATG1510.10757590869583802No Hit
AACGCAGGGTGTCACCACCTGGAACGTAGTTATCGGTCGGCAGGCTGAACAT1500.10686348545944176No Hit
TGCGCAGGGATGGGTCAAAGACCCTCTTGCTGAGCAGGCTACTGGAGCTGTC1490.10615106222304548No Hit
GAACAAGGGGAAATGGGCTGTCTTTCTTTTCCTTTCATGTAATGTCTGATTT1480.1054386389866492No Hit
CTAGCCGGGATCTAGGCAACTAAAAACAAACATTGTAAGAATGAATATTTTT1480.1054386389866492No Hit
AAGAATCGGGTAGTTACTTTGAATATACCATCTTCATTTTTGTAGCTTTTTA1470.1047262157502529No Hit
GTAGCTGGGTCACGCGGACCCAGAACCCCATGGTGACCGGGACCTCAGTCCT1470.1047262157502529No Hit
CTCAGATGGGGTAGATAGTTTTGTAGAAGACTAGAAATCAGTTTCTAAATGT1470.1047262157502529No Hit
TGTGCCTGGGCAAAAACAAAAGAAGCCTTCCTCTCACCTCATTTTGTAAATG1470.1047262157502529No Hit
CATCCAGGGCAATCATGAAGATACATAATTACAAACTGAGACAAGTGTCATA1470.1047262157502529No Hit
TGGCATGGGAGACCAATGATGAGTATTCTGGGGTGTCTGAATCAATGATTTT1470.1047262157502529No Hit
TATTGGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1460.10401379251385662No Hit
CTTGGCATGGGGCTCTTGTGCTGGTCTGTTCATGATATACTTTCAGGTAAGT1460.10401379251385662No Hit
TCAGTAGGGAGACATACATACAGCCAACTAGCGTGTTAAAGAAAACCTCAAT1460.10401379251385662No Hit
TTTGAGGGGGGAGATGGATGAGGACAAAGGTAATCCCCAACTCTGGCTATTT1460.10401379251385662No Hit
CTGCAGGGAATTGTGTGGCAGTGATTGGTCTGTTTGTGGAGAATGTATGAAA1460.10401379251385662No Hit
AAATGCGGGGAAGAAGAGGAATAAGAAAATCTTCGATAACTGGATGACGATC1450.10330136927746035No Hit
TAAAACGGGTTCTTCATAAATTGCAGTAACTTGAAAGCAGTTGGGAAATTAG1450.10330136927746035No Hit
ACTCTCGGGAGTGCATAGATCATTTAGTCCAAGAACTTAAAACTAAATAGAG1450.10330136927746035No Hit
TTGAGTGGGGTATAGATAGCCTGAAAAGCAATGTGAAAAGCAATGCTTCATC1450.10330136927746035No Hit
TTTTGGGGGATAACAGACCTGTAATAAATATTTATTGACTGAATGGACAAAG1450.10330136927746035No Hit
AAACAGGGGAAGTATTGCAGCCTTGTGCTATCCATTGTCCAGACACTGAAAA1450.10330136927746035No Hit
TTCACGCGGGATTACTATTTAGTTTTAAGCAATTTGTAGGCATGCATTTTTT1450.10330136927746035No Hit
AAGCGTGGGGGATCTCGTCAAGGGGCATAGATTCTGTGTAACTTTTTACTCT1440.10258894604106407No Hit
CAACCAGGGGGCAGGGAGGTGGGGAGATGCCCACCGATGCCTTCTGCTTGTG1440.10258894604106407No Hit
GTTCATGGGATTTGCAACAGTAATGAAGCCAATGAACTTGGAAGATGAAAAG1440.10258894604106407No Hit
TCACACGGGGCTCGCTGAGGGATTGGATGTATTTCACAGAATTTCCAACTGC1440.10258894604106407No Hit
GCGGTGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAAC1440.10258894604106407No Hit
GTAATAGGGGGAGGAGGGACAGGTAATGTGATTCTCCCTCCTCAACCTCCAC1440.10258894604106407No Hit
AGTTTTCCCAACACCATTTATTGAAGACCCTGTCCTTTTATACTTTTCAGTA1430.10187652280466779No Hit
TTCAAAGGGACCAGCCTGGCCAACGTGGTGAAACCCTGTCTCTACTAAAAAT1430.10187652280466779No Hit
AAAAACGGGGATTCACTAACTTTCTGTGCTAGATATAGTTCATGCTTAGCAT1430.10187652280466779No Hit
TAACTGCGGGCAGGTTGTGTTCATTCTTTTCCTGGAAATGATGACTTTATTG1420.10116409956827152No Hit
TAGCCTGGGTGACCAAAAAAGAAAAAGTACTGATCCTAGTCAGGCACTCTGC1420.10116409956827152No Hit
TTTTTAGGGATTTCACAAGTTATTTATTTATAGAATGAAAACTATGTAGTTA1410.10045167633187524No Hit
GCGTAGGGGGACAACAAAGCTCAAAGCCACTGTGGTTATCTGTCCTGTTGGG1410.10045167633187524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)