FastQCFastQC Report
Sat 31 Dec 2022
SRR5847048.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847048.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140207
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGC6110.4357842333121742No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGCCGTCT3330.23750597331089032No Hit
ACTGGTGGGGATCTAACTGAGGGACAAAAGGAAATGAAAAAGGAACTTAAAC2190.15619762208734228No Hit
TGATTAGGGAGCGGCTGCAACTGCGGCTCCTGGCCAGACCCCGGCCTCAGCG1930.13765361215916466No Hit
AGACTAGGGTCTCCCTAGTGCGGGCTGGCAGTGCGGGCAGAGCCCGGCTGAG1880.1340874564037459No Hit
GCGCAGGGAATCCTATGGGCTTGCAAATAAATAACTTACTTGTTCTCTTCAG1860.1326609941015784No Hit
TGAGATGGGACGTGATAATGACTTACTGTTTTGTTTTCATTATTTCCTCCTT1850.13194776295049462No Hit
TAAACTGGGACCAGGGAATTCTTACTGGCAAAATGATCAATCTGGAGTGTGC1780.12695514489290835No Hit
ACGATAGGGGGTGGTCCAAAGAAAACAAAATTGGGTTGAATGTTTCATTTAG1780.12695514489290835No Hit
GTTTCCGGGGAGGCAGCGAGAGTACGCGTGTGCCTCGCGCCGGTCCACGAGG1760.12552868259074085No Hit
TAATTCGGGAGACAGACTGGTTGGATGGCAAGCATGTTGTGTTCGGTCACGT1750.12481545143965707No Hit
TACTTAGGGACCTTATGCCCTGTTGAATTTTTGGTGTGAGATTTTTGAGTAA1740.12410222028857332No Hit
TTCGCCGGACGATCTATGCTGGGGAAGTAAAATGTGGAAAGAATTTAAATTA1720.12267575798640583No Hit
TCACACGGGGCTCGCTGAGGGATTGGATGTATTTCACAGAATTTCCAACTGC1710.12196252683532205No Hit
AGCAAGGGGGGCAGAAGGGCAGAGTGTGAAGCGATTGCTCACAATGGCAATC1710.12196252683532205No Hit
CACTTCGGGCTTTTTGCGAAGTGGGCTCGTGGGTTGGCAGTATGAGAGTTGT1700.1212492956842383No Hit
ATTGCAGGGAGAGGTGGAGTATGAACCCAGGTGTATCTCTAAAGACCATGAC1700.1212492956842383No Hit
CATTTGGGAGGAAGAAATGTGGTAAGACGTATACTTACATTTTCCTAAGACA1690.12053606453315455No Hit
AATCTTGGGGGGGGCCTTGTGGTTCTGCTGCCGCAGGATTGGCCAGTGAGAA1690.12053606453315455No Hit
TGATGTGGGGTGGGATGTGAGAGTTGGAAGACAGCTTGCGGGCCTGCTCCGC1680.11982283338207081No Hit
TGCGCAGGGATGGGTCAAAGACCCTCTTGCTGAGCAGGCTACTGGAGCTGTC1680.11982283338207081No Hit
TGTTAAGGGACTTTGGGAAGTTATTTCTTTATTTTAATGATGTATCATTGTT1660.11839637107990328No Hit
TTCGCTGGGGATACATTTTATCCTTTTCTCATTTGCTGGGTGGGGGAAGTGT1640.11696990877773578No Hit
CTAGCCGGGATCTAGGCAACTAAAAACAAACATTGTAAGAATGAATATTTTT1610.11483021532448451No Hit
TGGCGTGGGAGATTGGCAGTCTCTTTCGCTTATCTGCTGTTTTAAAGAATTC1610.11483021532448451No Hit
TCAGCTGGGGGATAGGTAAGATATGATAGAGCATAACAGGATAAGAAGAAAG1610.11483021532448451No Hit
GCCTTGGGGAGGGGTCAAGAATATAAACATACTCAATTGCTACAGCAGGAGG1600.11411698417340076No Hit
GCTGGGGGCCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA1580.11269052187123324No Hit
GCACGAGCGTAAGGTCCAGCTGGTCACCGACAGGGATCACTTCATCCGAACC1570.11197729072014949No Hit
ATAGCAGGGCACCCAGTGGCTGGCGCTGTGGCTGGCTCCATAGATGTCGCAC1560.11126405956906574No Hit
TTTCATGGGCAAACCGCTGGTGAAGGAGGGAGGCGACCGCCCTCGTCACGTC1560.11126405956906574No Hit
CAGACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1560.11126405956906574No Hit
TCAGAGGGGCGTTGTTAATTAACCTTTCAGTAACAATAATAGATGGGGTCTG1550.11055082841798199No Hit
CTAAAAGGGGAAACATTGTCAAAGAAAGAAGGTGTGGTGCAAAGACTTCTGC1550.11055082841798199No Hit
TAAAACGGGTTCTTCATAAATTGCAGTAACTTGAAAGCAGTTGGGAAATTAG1540.10983759726689822No Hit
CAATACGGGCGATCTTTCGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCT1540.10983759726689822No Hit
AAGCGTGGGGGATCTCGTCAAGGGGCATAGATTCTGTGTAACTTTTTACTCT1530.10912436611581447No Hit
TAAGTGGGGTTAATTGAGAGGTGAGAACATAAGATTCCTCTGGAAATTTGCT1530.10912436611581447No Hit
GTTCATGGGATTTGCAACAGTAATGAAGCCAATGAACTTGGAAGATGAAAAG1520.10841113496473072No Hit
AAGTCTGGGGGGCTAATTGATGAGCATCTTATTTCTTATCTCTAAAAATTAT1510.10769790381364697No Hit
TTAGTAGGGAGAGGAGGAGCGGCTCTGTGAAAGCTGGAGGGCTCACACATTA1510.10769790381364697No Hit
CTGCAAGGGGAATCCAGAATTGGGCCAAATCTCAGCAGGCTTTGTGTCCTTC1510.10769790381364697No Hit
ACAAGAGGGAGTACCATAAGAGCATCACCCCCAATTTTTCTTTGAAAGATGT1510.10769790381364697No Hit
TGTGCCTGGGCAAAAACAAAAGAAGCCTTCCTCTCACCTCATTTTGTAAATG1510.10769790381364697No Hit
AGTTTAGGGAACCACAGCAGTGCTCAATTGTGGCGAATTACTTCTCTATCCC1500.1069846726625632No Hit
GCATTAGGGGGTCCCCAAACAACCACTTAGAGGCTAGCTGGATATCCTGCTG1500.1069846726625632No Hit
TTTTTAGGGATTTCACAAGTTATTTATTTATAGAATGAAAACTATGTAGTTA1500.1069846726625632No Hit
ATCCTAGGGGCTTGGGAAGATGGCGGCTGTGTCGGTGTATGCTCCACCAGTT1500.1069846726625632No Hit
GAATCGGGGGCCAGTCTGTTGTGGGCATTGTTTTCTACAACCAAATTCTGGG1500.1069846726625632No Hit
CGACCGGGATGGGAATCAGAATATTGGCTTCAATCACTGACTCATCTTTATA1500.1069846726625632No Hit
GTAGCTGGGTCACGCGGACCCAGAACCCCATGGTGACCGGGACCTCAGTCCT1490.10627144151147945No Hit
CGCTCAGGGGCACCCCGAGGTGTTCCTCAGATTGAAGTCACTTTTGACATTG1490.10627144151147945No Hit
TATTCTGGGATTGAATGTAAATGAACTAAACTCTCCAATTAAAAGACAGAAT1480.1055582103603957No Hit
CGCTGTGGGGGATCCTGTAAATTCTAAGATTTTAAAAGGATTTTTGCTTATG1480.1055582103603957No Hit
TCATACGGGATCTTAAAACTCGATCACCAGTCACTTTCCTGTCTGATCTGCG1480.1055582103603957No Hit
TTCACGCGGGATTACTATTTAGTTTTAAGCAATTTGTAGGCATGCATTTTTT1470.10484497920931195No Hit
GCAACCGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1470.10484497920931195No Hit
TAATTAGGGCTGATGAGACTAGAGTGACAGTGTTTCAGAACCCAAATGTCCT1470.10484497920931195No Hit
AAAAATGGGTAGGTTTTTTAAACAGAATCCTACTGTTAATGGTAATAACTTC1470.10484497920931195No Hit
AAGCAAGGGATCTCATGAAAAATGAATCTCATGGAAATATTGCCTTATTTAT1460.10413174805822818No Hit
AAACAGGGGGCTTTCCTTCTTGTTTGAATATATTGGTGAAATGGGAAAAGAC1460.10413174805822818No Hit
CTTGACACGGGACACAGTAAAGTCGACAAACAAGCATCGCGAATGCACAAAA1450.10341851690714443No Hit
TTTATAGGGGACTTCTCGGTGCTAGTTGGCCATCTTGACTCAATGTTGGGAT1450.10341851690714443No Hit
GGACATGGGCAGGACTCACATGGATATAGTTTCTGTCTCCCTCTCTAATTGT1450.10341851690714443No Hit
TAGCCTGGGTGACCAAAAAAGAAAAAGTACTGATCCTAGTCAGGCACTCTGC1450.10341851690714443No Hit
TTTAAGGGGCAGCGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTG1450.10341851690714443No Hit
TAACCAGGGCAGATACAAAGGTGCCAAGATCCAGCTCCTGGATCTCCCAGGT1440.10270528575606068No Hit
CTCACAGGGAGTACTTTAGACTAATTGTGTTTGTCCGTCCATGCCATGAATG1440.10270528575606068No Hit
AAAAACGGGGATTCACTAACTTTCTGTGCTAGATATAGTTCATGCTTAGCAT1440.10270528575606068No Hit
ACACAGGGGGGCGGCAACCGACGTACACAAGGGGCTTGAGCGTTCTGTGGAG1440.10270528575606068No Hit
CTGAGGGACGAGGATGAAGTAGAGGATATGGAAGAAAATTAAGTGTGATCCA1430.10199205460497693No Hit
GATCTAGGGTAGTTTCATCACATTTACTTCATCATGTTCACTTGTAAGCCTG1430.10199205460497693No Hit
ACAAAAGGGATTCTGGGCTGGAGAATTCTTTGTTTTCAGGGGTTGTCTTGAA1420.10127882345389318No Hit
CACTTAGGGGGTTTCGAACTCCTGACCTCAAGTGATCCACCTGCCTCGGCCT1420.10127882345389318No Hit
TGGCATGGGAGACCAATGATGAGTATTCTGGGGTGTCTGAATCAATGATTTT1420.10127882345389318No Hit
CGTTACGGGTGCCAGTAACCTGTAGAGTTAGCACTTCAACTTTCTTTAGCGC1410.10056559230280941No Hit
ACCTGGGGGCTGTAATTTTCACTAACAAACACTGCCAGTTGTTTGCCTTGAA1410.10056559230280941No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)